[Histonet] RE: Eosin staining for small biopsies

Morken, Timothy Timothy.Morken <@t> ucsfmedctr.org
Wed Jun 20 10:29:28 CDT 2012

Here is a paper I found:

Capillary Electrophoresis Artifact Due to Eosin
Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005

Our molecular people are studying this issue to see how big of a problem it is.

Tim Morken
Supervisor, Electron Microscopy
Department of Pathology
UC San Francisco Medical Center
tim.morken <@t> ucsfmedctr.org

Tim Morken

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Freeman, Carol
Sent: Wednesday, June 20, 2012 6:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin staining for small biopsies

Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First does anyone have any papers written or studies done on the use of Eosin to stain small biopsies or the use of eosin on the tissue processor in the last alcohol??  I have read snippets on eosin having an effect on FISH testing?? Does anyone know of this to be true and have any papers or studies to refer back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies and/or any thoughts on its use to do so?? My thoughts are that because of it's precipitation qualities it could gunk up the tissue processor and leave residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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