[Histonet] RE: Eosin staining for small biopsies

Lynette Pavelich LPaveli1 <@t> hurleymc.com
Wed Jun 20 09:11:41 CDT 2012

We also add liquid eosin (~75mL) to our "dirtiest" 100% to help aide the sight of the small biopsies for embedding. You are correct though, that eosin does fluoresce. 

I understand why you would be concerned about FISH, so wanted to share this. I have heard from fellow techs in the field that use a common bluing agent (used for laundry!) called Mrs. Stewart's Concentrated Liquid Bluing (found locally at a discount store called Meijers).  It is applied at the grossing board. 
These techs actually use it for skin, but the bluing withstands the processor and helps them embed the skins much easier by showing the epidermis. I do believe that this harmless liquid does not fluoresce.

Hope this helps,


Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966

From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Freeman, Carol [Carol.Freeman <@t> utoledo.edu]
Sent: Wednesday, June 20, 2012 9:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin staining for small biopsies

Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First
does anyone have any papers written or studies done on the use of Eosin
to stain small biopsies or the use of eosin on the tissue processor in
the last alcohol??  I have read snippets on eosin having an effect on
FISH testing?? Does anyone know of this to be true and have any papers
or studies to refer back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies
and/or any thoughts on its use to do so?? My thoughts are that because
of it's precipitation qualities it could gunk up the tissue processor
and leave residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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