[Histonet] RE: Antigen retrieval

Elizabeth Chlipala liz <@t> premierlab.com
Wed Jun 13 09:32:56 CDT 2012


Retrieval methods are also based upon the antibody.  During protocol development we try several different retrieval methods - no retrieval, enzymatic (proteinase K), pH6 and pH9, we then determine the best method and go from there.  One retrieval method may not work for all antibodies.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Wednesday, June 13, 2012 8:27 AM
To: histonet <@t> lists.utsouthwestern.edu (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Antigen retrieval

Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help!

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