[Histonet] RE: Reagent rotation

vijaykanthg <@t> orchidpharma.com vijaykanthg <@t> orchidpharma.com
Wed Jun 6 23:20:36 CDT 2012


Dear Mark

We change the solution depend upon no of cassettes run. In your case the size of specimen is small so u can change/ rotate depend upon no of run.    i felt that the size of the specimen make big impact on reagents in Tissue Processer.

With regards
Dr G vijay
Orchid Pharma
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, June 06, 2012 10:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 103, Issue 7

Send Histonet mailing list submissions to
        histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
        histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Reagent rotation (Mark Perrin)
   2. RE: Reagent rotation (Goins, Tresa)
   3. Reagent rotation (Stephenson, Sheryl)
   4. Re: Fluorescence-filters (Eric Hoy)
   5. Current Histology Openings (Brannon Owens)
   6. Re: Fluorescence-filters (Mark Tarango)
   7. NEW Position Alert - Quality Assurance Histologist (Matt Ward)
   8. Re: Fluorescence-filters (Emily Sours)
   9. Re: Fluorescence-filters (Emily Sours)
  10. RE: Reagent rotation (Ian R Bernard)
  11. RE: Fluorescence-filters (Jonathan Cremer)
  12. RE: Fluorescence-filters (Sue Hunter)
  13. Histotech scheduling (Duddey, Aimee)
  14. RE: Histotech scheduling (joelle weaver)
  15. RE: Histotech scheduling (Fahy, Claire)
  16. staffing questions (Hutton, Allison)
  17. Re: staffing questions (Rene J Buesa)


----------------------------------------------------------------------

Message: 1
Date: Tue, 5 Jun 2012 11:04:50 -0700 (PDT)
From: Mark Perrin <perrintoshia <@t> yahoo.com>
Subject: [Histonet] Reagent rotation
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1338919490.15637.YahooMailNeo <@t> web44714.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies.

Thanks in advance for your input.

?
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it.

------------------------------

Message: 2
Date: Tue, 5 Jun 2012 18:35:14 +0000
From: "Goins, Tresa" <TGoins <@t> mt.gov>
Subject: RE: [Histonet] Reagent rotation
To: 'Mark Perrin' <perrintoshia <@t> yahoo.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CA4DF32ED505D94BB55E95487D8E98411F24C0CD <@t> DOAISD5205.state.mt.ads>
Content-Type: text/plain; charset="iso-8859-1"

We rotate by cassette number - our daily load can vary from 10 to more than 100 cassettes, so an exchange schedule based on number of runs was not appropriate.

Tresa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies.

Thanks in advance for your input.

?
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 3
Date: Tue, 5 Jun 2012 14:02:36 -0500
From: "Stephenson, Sheryl" <SStephenson <@t> lifecell.com>
Subject: [Histonet] Reagent rotation
To: 'Mark Perrin' <perrintoshia <@t> yahoo.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C2A8FE4DA0ED8C468A3713F8607829D30D3F922461 <@t> AMWPVEX03.kci.com>
Content-Type: text/plain; charset="iso-8859-1"


We do it by the number of Runs.  About every 6 run for rotations and 12 runs for a full change out.   But we also have another processor as well.

Sheryl Stephenson | Histology Technician

       Main 908.947.1100                     Fax        908.947.1085
 Direct:  908.947.1624    ?sstephenson <@t> lifecell.com
 732. 939. 3037  Cell                 www.lifecell.com

LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876


?


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies.

Thanks in advance for your input.

?
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 4
Date: Tue, 05 Jun 2012 15:30:19 -0500
From: Eric Hoy <Eric.Hoy <@t> UTSouthwestern.edu>
Subject: Re: [Histonet] Fluorescence-filters
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CBF3D68B.25857%Eric.Hoy <@t> UTSouthwestern.edu>
Content-Type: text/plain;       charset="US-ASCII"

We do a LOT of fluorescent microscopy in our immunology lab, so I have a bit
of experience with fluorescence.

The answer to your question depends on what type of filters you have in your
microscope, and what type of light source is on the microscope.

Older fluorescent systems used absorption filters, which were simply discs
of coloured glass.  These filters had a fairly wide band-pass, so the
fluorescence tended to be less than we see with interference filters.  The
good news with these filters is that they are nearly indestructible (unless
you drop and break them.)

Interference filters are produced by vacuum deposition of a thin film of
metal vapour on high-quality glass.  These filters usually have much sharper
band pass characteristics than absorption filters.  They are also
considerably more expensive.  If handled properly, these filters will last
for decades, but improper cleaning and handling of the filters can shorten
their lifespan.  I have also heard that prolonged exposure to solvent
vapours (such as we find in a histology lab), can damage the filters,
although I have not seen any filters that suffered this type of damage.

I have seen interference filters that show "delamination" of the metal film
over time.  In my experience these are older filters that were not produced
with the current technologies, and filters that have been mishandled.
Interference filters made in the past 20 years should last as long as the
microscope, if they are properly handled.

If you are seeing reduced fluorescence, I would suspect the light source as
the most likely problem.  Halogen lamps have less intensity than mercury
vapour lamps, which are less intense than metal halide lamps, which are less
intense than LED sources.  We have converted all of our microscopes to LED
sources.  If you are using an older HBO or halogen lamp, the age of the
lamp, the initial wattage of the lamp, and the alignment can all affect the
fluorescent output.

As you identified, perhaps the most important aspect of immunofluorescence
is the skill and experience of the person who reads the slides.

Let me know if you have further questions.

Eric Hoy

===============================================
Eric S. Hoy, Ph.D., SI(ASCP)
Clinical Associate Professor
Department of Medical Laboratory Sciences
The University of Texas Southwestern Medical Center
Dallas, Texas
Email: Eric.Hoy <@t> UTSouthwestern.edu
===============================================

On 6/5/12 9:37 AM, "Gudrun Lang" <gu.lang <@t> gmx.at> wrote:

> Hi!
>
> Filters for fluorescencemicroscopy tend to "burn out" after a certain
> duration of usage. What duration?
>
> We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours
> are about 150 per year.
>
>
>
> What do you think? Is it time to change them.
>
> I have often bad feedback about weak signals, and I would not be surprised
> if the microscope is the culprit and not our protocol.
>
>
>
> Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but
> tumourcells mixed within collagenfibers.
>
> - and unfortunately unexperienced doctors on reading of this special probe.





------------------------------

Message: 5
Date: Tue, 05 Jun 2012 17:02:34 -0400
From: Brannon Owens <brannon <@t> alliedsearchpartners.com>
Subject: [Histonet] Current Histology Openings
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CBF3EC2A.14D00%brannon <@t> alliedsearchpartners.com>
Content-Type: text/plain;       charset="US-ASCII"

Allied Search Partners is seeking qualified candidates for the following
openings in Histology.
1)  Night time Histology Supervisor- North of Los Angeles, CA
2)  Histology Manager- Fort Myers, FL
3)  Part time Mohs Tech- Denver,  CO
4)  IHC Technologist- Port Chester, NY

Email/message me for full job descriptions!
--
Brannon Owens
Recruitment Manager
Allied Search Partners


T: 888.388.7571 ext. 106

F: 888.388.7572






------------------------------

Message: 6
Date: Tue, 5 Jun 2012 14:47:58 -0700
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Fluorescence-filters
To: Eric Hoy <Eric.Hoy <@t> utsouthwestern.edu>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CACJwj1KSFtVhkr=J5s_EYKONyBJkVn6OU4+U_nbG18tV-LvONA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi Gudrun,

i read about 15 ALK cases a week.  If you are seeing a lot of collagen
fibers around the tumor cells, I'd try increasing the digestion time of
your pepsin (especially if they were fixed for longer than usual).  Before
altering the pretreatment though, you would want to make sure that these
slides were not exposed to light for any period of time.  The discrete
signals of the probes can quickly fade (much faster than with IF stained
slides).  I'd also make sure the door is closed in your dark room.  If
there is light in the corner of your eye the signals can be hard to see.
 ALK FISH should be scored at high power under oil immersion (60-100x
objective).  If they are scoring at 40x, it could be a problem.

good luck!

Mark Tarango

On Tuesday, June 5, 2012, Eric Hoy wrote:

> We do a LOT of fluorescent microscopy in our immunology lab, so I have a
> bit
> of experience with fluorescence.
>
> The answer to your question depends on what type of filters you have in
> your
> microscope, and what type of light source is on the microscope.
>
> Older fluorescent systems used absorption filters, which were simply discs
> of coloured glass.  These filters had a fairly wide band-pass, so the
> fluorescence tended to be less than we see with interference filters.  The
> good news with these filters is that they are nearly indestructible (unless
> you drop and break them.)
>
> Interference filters are produced by vacuum deposition of a thin film of
> metal vapour on high-quality glass.  These filters usually have much
> sharper
> band pass characteristics than absorption filters.  They are also
> considerably more expensive.  If handled properly, these filters will last
> for decades, but improper cleaning and handling of the filters can shorten
> their lifespan.  I have also heard that prolonged exposure to solvent
> vapours (such as we find in a histology lab), can damage the filters,
> although I have not seen any filters that suffered this type of damage.
>
> I have seen interference filters that show "delamination" of the metal film
> over time.  In my experience these are older filters that were not produced
> with the current technologies, and filters that have been mishandled.
> Interference filters made in the past 20 years should last as long as the
> microscope, if they are properly handled.
>
> If you are seeing reduced fluorescence, I would suspect the light source as
> the most likely problem.  Halogen lamps have less intensity than mercury
> vapour lamps, which are less intense than metal halide lamps, which are
> less
> intense than LED sources.  We have converted all of our microscopes to LED
> sources.  If you are using an older HBO or halogen lamp, the age of the
> lamp, the initial wattage of the lamp, and the alignment can all affect the
> fluorescent output.
>
> As you identified, perhaps the most important aspect of immunofluorescence
> is the skill and experience of the person who reads the slides.
>
> Let me know if you have further questions.
>
> Eric Hoy
>
> ===============================================
> Eric S. Hoy, Ph.D., SI(ASCP)
> Clinical Associate Professor
> Department of Medical Laboratory Sciences
> The University of Texas Southwestern Medical Center
> Dallas, Texas
> Email: Eric.Hoy <@t> UTSouthwestern.edu
> ===============================================
>
> On 6/5/12 9:37 AM, "Gudrun Lang" <gu.lang <@t> gmx.at <javascript:;>> wrote:
>
> > Hi!
> >
> > Filters for fluorescencemicroscopy tend to "burn out" after a certain
> > duration of usage. What duration?
> >
> > We have filters for FITC, TRITC, Dapi and a triplefilter. The
> working-hours
> > are about 150 per year.
> >
> >
> >
> > What do you think? Is it time to change them.
> >
> > I have often bad feedback about weak signals, and I would not be
> surprised
> > if the microscope is the culprit and not our protocol.
> >
> >
> >
> > Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed
> but
> > tumourcells mixed within collagenfibers.
> >
> > - and unfortunately unexperienced doctors on reading of this special
> probe.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 7
Date: Tue, 5 Jun 2012 18:27:32 -0400
From: Matt Ward <mw <@t> personifysearch.com>
Subject: [Histonet] NEW Position Alert - Quality Assurance Histologist
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <855477cea9188eab486251231404faa7 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Good Evening Histonet,



We are currently searching for a Histotech that would be interested in a QA
role with a global leader in Histology based in Richmond IL. The company is
growing and offers a strong package.



Please respond to this e-mail with your resume if you would be interested
in learning more.



*Quality Assurance Histologist *





*The Company:*



Well-established provider of consumables and medical device accessories for
clinical histology and research laboratories.  The facility works closely
with our UK, German and Australian facilities in the development,
manufacturing and marketing of products including processing reagents,
storage and specimen transport devices, cytology accessories and safety
products.



This is a globally focused business with significant sales and operations
in the US, Europe and Asia Pacific as well as a direct presence in over 100
countries.



*The Opportunity:*



The company currently has an opening for a Quality Assurance Histologist to
be based in Richmond IL. This position reports to the Quality Assurance
Manager.  All applicants must not be adverse to travel, as this is a
position that may require domestic and international travel when necessary.



Salary: Commensurate with experience



Other: Full benefits - 401k program/matching



*Primary Responsibilities: *



The primary purpose for this position is to perform product specification
and functionality testing on incoming raw material and final products to
release for distribution.



Additional Responsibilities:

- Perform incoming, in process and final inspection on product for release

- Effectively communicate the status of product testing

- Assist in the maintenance of laboratory facilities, equipment and
consumables

- Perform additional testing and investigation related to customer
complaints

- Maintain laboratory equipment

- Good documentation practices

- Necessary computer skills, Microsoft Word, Excel, and Powerpoint. Use of
SAP is preferable

- A commitment to follow standard company financial procedures

- Achieve best practice safety performance levels

- Product training and quality systems training



*Education and Experience Required:*



- Ability to work independently and as part of a team

- Able to perform tissue grossing, tissue processing, embedding

- Sectioning paraffin embedded tissue as well as frozen tissue

- Performing routine stains (H and E) as well as special stains

- Formulation and production of routine laboratory reagents and solutions

- Performing and documenting routine laboratory procedures

- Familiarity with compliance requirements in the medical device industry

- Good communication skills both verbal and written

- Proficiency in basic computer skills and with software applications such
as Microsoft Office



Regards,



Matt Ward

*Account Executive*

*Personify*

5020 Weston Parkway Suite 315

Cary NC 27513

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

 www.personifysearch.com


------------------------------

Message: 8
Date: Tue, 5 Jun 2012 19:27:34 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Fluorescence-filters
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAP=XX1wKNmEhaP2xCqncPZ4zJgUDbzMM9tsH_6wSy7pTqdjgDg <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Wow, that was detailed and interesting!!
I didn't know microscopes could even use LED, does that require a different
setup, or just a different bulb?

Emily

"You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir."
--Howard Moon, in "Charlie", The Mighty Boosh


------------------------------

Message: 9
Date: Tue, 5 Jun 2012 19:31:39 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Fluorescence-filters
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAP=XX1xQ-n3mdUwODD=kFjfaanQoBrN4vzEcLrPw5T4t1ZEWJw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).

Emily

"You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir."
--Howard Moon, in "Charlie", The Mighty Boosh


------------------------------

Message: 10
Date: Wed, 6 Jun 2012 01:22:40 +0000
From: Ian R Bernard <ibernard <@t> uab.edu>
Subject: RE: [Histonet] Reagent rotation
To: "Stephenson, Sheryl" <SStephenson <@t> lifecell.com>, 'Mark Perrin'
        <perrintoshia <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <D4F4C602B10B9F45B4E9271AF6380E1614148899 <@t> UABEXMB4.ad.uab.edu>
Content-Type: text/plain; charset="iso-8859-1"

We do it by number of blocks- At or after 350 blocks the processor is changed.

Ian

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl
Sent: Tuesday, June 05, 2012 2:03 PM
To: 'Mark Perrin'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation


We do it by the number of Runs.  About every 6 run for rotations and 12 runs for a full change out.   But we also have another processor as well.

Sheryl Stephenson | Histology Technician

       Main 908.947.1100                     Fax        908.947.1085
 Direct:  908.947.1624    ?sstephenson <@t> lifecell.com
 732. 939. 3037  Cell                 www.lifecell.com

LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876


?


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Perrin
Sent: Tuesday, June 05, 2012 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reagent rotation

What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies.

Thanks in advance for your input.

?
Toshia Perrin
Medical Practice Coordinator
Southern Pathology

This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 11
Date: Wed, 6 Jun 2012 06:18:00 +0000
From: Jonathan Cremer <Jonathan.Cremer <@t> med.kuleuven.be>
Subject: RE: [Histonet] Fluorescence-filters
To: Emily Sours <talulahgosh <@t> gmail.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C35C2709C22FC2458D6A918FACC338A30C612FAA <@t> ICTS-S-MBX5.luna.kuleuven.be>

Content-Type: text/plain; charset="us-ascii"

Sure you can, all you need is a bright enough lightsource which emits at the correct wavelength. If the spectrum of the light source is too wide, you dump in a filter to narrow it down to your desired excitation.
Flow cytometers nowadays even use LED lasers instead of the huge, bulky and vulnerable gas lasers of the old days. Which is a good thing.

Jonathan
---
Jonathan Cremer
Laboratory Technician
TARGID - KU Leuven

________________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] namens Emily Sours [talulahgosh <@t> gmail.com]
Verzonden: woensdag 6 juni 2012 1:31
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: Re: [Histonet] Fluorescence-filters

Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for
brightfield).

Emily

"You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir."
--Howard Moon, in "Charlie", The Mighty Boosh
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 12
Date: Wed, 6 Jun 2012 11:39:54 +0000
From: Sue Hunter <SHUNTER <@t> beaumont.edu>
Subject: RE: [Histonet] Fluorescence-filters
To: Emily Sours <talulahgosh <@t> gmail.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <B493B3E4EF638A41875845CEDF938B44014EA02B <@t> EXMail04.ms.beaumont.edu>
Content-Type: text/plain; charset="utf-8"

We recently installed a LED light source on our fluorescence scope that our pathologists use for reading IF on kidneys and skins.  They love it.  No more having to warm up the bulb, not turning it off too soon,  or my nightmare - leaving it on overnight.  The bulb is supposed to last a really long time and gives off a nice bright light.

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, June 05, 2012 7:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Fluorescence-filters

Can you do fluorescence with LED?
(That may be a stupid question, as I always thought LED was just for brightfield).

Emily

"You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir."
--Howard Moon, in "Charlie", The Mighty Boosh _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
To report this email as SPAM, please forward it to spam <@t> websense.com.

------------------------------

Message: 13
Date: Wed, 6 Jun 2012 08:30:47 -0400
From: "Duddey, Aimee" <ADuddey <@t> firsthealth.org>
Subject: [Histonet] Histotech scheduling
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <A325E5C712710D4C82EA9CA1226466B823CBAAC3 <@t> EVS1-FHC.firsthealth.org>
Content-Type: text/plain;       charset="us-ascii"

I am looking for some creative scheduling ideas.  We have a day shift
crew that starts at 4am and goes til 530pm.  The schedule is below.
Techs would like to rotate to do different jobs within the department
but don't really want to rotate hours.  Can I please get some examples
of how others of you are staffing?  Even if you have a second or third
shift I would like to see some different workflow plans and how they are
staffed.



1 HT 400am - Embedder

2 HTs 500am - Cutters

1 HT 730 - Immunos/specials/orders

1 HT 830 - Grossing/pathologist assist

1 lab assistant 800am - cytology prep/gross room assist



Aimee M. Duddey, MLT(ASCP)

Moore Regional Hospital Laboratory

Assistant Director of Laboratory - Pathology

910-715-5286

FirstHealth OF THE CAROLINAS





------------------------------

Message: 14
Date: Wed, 6 Jun 2012 14:14:05 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] Histotech scheduling
To: <aduddey <@t> firsthealth.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT135-W383F2EDBAD7678B497EF1D80D0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


http://www.biomedcentral.com/1472-6890/10/2




Joelle Weaver MAOM, HTL (ASCP) QIHC
 > Date: Wed, 6 Jun 2012 08:30:47 -0400
> From: ADuddey <@t> firsthealth.org
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histotech scheduling
>
> I am looking for some creative scheduling ideas.  We have a day shift
> crew that starts at 4am and goes til 530pm.  The schedule is below.
> Techs would like to rotate to do different jobs within the department
> but don't really want to rotate hours.  Can I please get some examples
> of how others of you are staffing?  Even if you have a second or third
> shift I would like to see some different workflow plans and how they are
> staffed.
>
>
>
> 1 HT 400am - Embedder
>
> 2 HTs 500am - Cutters
>
> 1 HT 730 - Immunos/specials/orders
>
> 1 HT 830 - Grossing/pathologist assist
>
> 1 lab assistant 800am - cytology prep/gross room assist
>
>
>
> Aimee M. Duddey, MLT(ASCP)
>
> Moore Regional Hospital Laboratory
>
> Assistant Director of Laboratory - Pathology
>
> 910-715-5286
>
> FirstHealth OF THE CAROLINAS
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 15
Date: Wed, 6 Jun 2012 15:19:46 +0100
From: "Fahy, Claire" <Claire.Fahy <@t> agriculture.gov.ie>
Subject: RE: [Histonet] Histotech scheduling
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <DB0ABCF605B6EB40920E18C2B01B2B48033916780B34 <@t> SDBE2K7CCRV03.agriculture.gov.ie>

Content-Type: text/plain; charset="utf-8"

Please unsubscribe me.
thanks

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: 06 June 2012 15:14
To: aduddey <@t> firsthealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Histotech scheduling


http://www.biomedcentral.com/1472-6890/10/2




Joelle Weaver MAOM, HTL (ASCP) QIHC
 > Date: Wed, 6 Jun 2012 08:30:47 -0400
> From: ADuddey <@t> firsthealth.org
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histotech scheduling
>
> I am looking for some creative scheduling ideas.  We have a day shift
> crew that starts at 4am and goes til 530pm.  The schedule is below.
> Techs would like to rotate to do different jobs within the department
> but don't really want to rotate hours.  Can I please get some examples
> of how others of you are staffing?  Even if you have a second or third
> shift I would like to see some different workflow plans and how they
> are staffed.
>
>
>
> 1 HT 400am - Embedder
>
> 2 HTs 500am - Cutters
>
> 1 HT 730 - Immunos/specials/orders
>
> 1 HT 830 - Grossing/pathologist assist
>
> 1 lab assistant 800am - cytology prep/gross room assist
>
>
>
> Aimee M. Duddey, MLT(ASCP)
>
> Moore Regional Hospital Laboratory
>
> Assistant Director of Laboratory - Pathology
>
> 910-715-5286
>
> FirstHealth OF THE CAROLINAS
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
                                          _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Department of Agriculture, Food and the Marine

The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege.  If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s).

An Roinn Talmha??ochta, Bia agus Mara

T?? an t-eolais san r??omhphost seo, agus in aon ceangl??in leis, faoi phribhl??id agus faoi r??n agus le h-aghaigh an seola?? amh??in. D???fh??adfadh ??bhar an seoladh seo bheith faoi phribhl??id profisi??nta n?? dl??thi??il. Mura tusa an seola?? a bh?? beartaithe leis an r??omhphost seo a fh??il, t?? cosc air, n?? aon chuid de, a ??s??id, a ch??ipe??l, n?? a scaoileadh.  M?? th??inig s?? chugat de bharr dearmad, t??igh i dteagmh??il leis an seolt??ir agus scrios an t-??bhar ?? do r??omhaire le do thoil.

------------------------------

Message: 16
Date: Wed, 6 Jun 2012 11:09:06 -0400
From: "Hutton, Allison" <AHutton <@t> dh.org>
Subject: [Histonet] staffing questions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <38A56C4F4630D348A50B3720409270870E0FE5D6 <@t> dhmail.dhorg.org>
Content-Type: text/plain;       charset="iso-8859-1"

I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs.  If anybody is willing to answer the questions listed below, I would appreciate your input.  You may email me at privately and all information will be kept confidental.
1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides
2. Approximate workload, either blocks per day or cases per year
3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful)
Thank You in advance
Allison Hutton, HTL(ASCP)cm
Lead Tech Histology
Doylestown Hospital
ahutton <@t> dh.org



------------------------------

Message: 17
Date: Wed, 6 Jun 2012 08:33:57 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] staffing questions
To: "Hutton, Allison" <AHutton <@t> dh.org>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1338996837.66141.YahooMailNeo <@t> web121402.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Staff level (your question 1) depends on the answer to your question 2 and also depends on the productivity level, both individual and collective.
The salaries depend on the nature of your lab (non for profit, private for profit or governmental) and with the total benefits offered. But also depend on the market availability and the willingness to negotiate both from the administration and the potential or existing employees.
So you ca understand both issues I am sending you some information under separate cover.
Ren? J.


________________________________
From: "Hutton, Allison" <AHutton <@t> dh.org>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, June 6, 2012 11:09 AM
Subject: [Histonet] staffing questions

I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs.? If anybody is willing to answer the questions listed below, I would appreciate your input.? You may email me at privately and all information will be kept confidental.
1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides
2. Approximate workload, either blocks per day or cases per year
3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful)
Thank You in advance
Allison Hutton, HTL(ASCP)cm
Lead Tech Histology
Doylestown Hospital
ahutton <@t> dh.org

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 103, Issue 7
****************************************





More information about the Histonet mailing list