[Histonet] Special Stain query

[Rene J Buesa]

Reagents:
Sol.A = water 30mL + 95%EthOL 70mL + HCl 0.6mL + orcein 1 g
Sol.B = HCl 0.3mL + absolute EthOL 97mL
Sol.C = Giemsa solution (0.05% eosin + 0.8% azur 5) 0.6mL + water 100mL
Sol.D = 0.001% eosin Y in 95%EthOL
 
Method:
sections → 70%EthOL → A x ½ to 1 hour → rinse → 95% EthOL a few dips → 100% EthOL til decolorized to pale brwon → B till almost completely decolorized → wash → C x 2 to 12 hours → blot dry → D till dehydrate → clear → mount
 
Recommended for skin section.
Ref.: Peter Gray pp 424 Method 21.412 Pinkus 1944
There you have it!
René J.

--- On Fri, 6/1/12, Chiriboga, Luis <Luis.Chiriboga <@t> nyumc.org> wrote:


From: Chiriboga, Luis <Luis.Chiriboga <@t> nyumc.org>
Subject: [Histonet] Special Stain query
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, June 1, 2012, 2:17 PM


Does anyone have a method/protocol for Pinkus’acid orcein-giemsa stain?
Thanks and good weekend to all


Luis Chiriboga
Experimental Pathology Core Laboratory
New York University School of Medicine



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