[Histonet] Re: ZN control making procedure inhouse
naveeda arshad
naveedafahim <@t> yahoo.ca
Mon Jul 23 19:32:26 CDT 2012
Hi All does any one has procedure for making inhouse ZN control if they can share with me . I will appreciate itthanksNaveeda
--- On Mon, 7/23/12, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 104, Issue 26
To: histonet <@t> lists.utsouthwestern.edu
Received: Monday, July 23, 2012, 8:30 PM
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Today's Topics:
1. disposable blade holder (Patsy Ruegg)
2. Secondary antibody causing nuclear staining? (Eva Permaul)
3. RE: disposable blade holder (Bea DeBrosse-Serra)
4. RE: disposable blade holder (Patsy Ruegg)
5. RE: Negative Reagent Control (Smith, Allen)
6. counter stain for PAS for fungus (Michele Carr)
7. Re: counter stain for PAS for fungus (Rene J Buesa)
8. Re: counter stain for PAS for fungus (Rena Fail)
----------------------------------------------------------------------
Message: 1
Date: Sun, 22 Jul 2012 12:33:01 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] disposable blade holder
To: <Histonet <@t> lists.utsouthwestern.edu>
Cc: 'Sherri Saturley' <ssaturley <@t> flagshipbio.com>
Message-ID: <821D0154318D4F64AD99824B87FB2DB0 <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
We are looking for the disposable blade holder for low profile blades that
fit in microtomes like the Leica 1510 where permanent blades used to be
held. We have two of these but they are both for high profile blades and we
would prefer to use low profile disposable blades. Does anyone have one of
these sitting around used we could buy from you or if there are vendors
selling used ones please feel free to contact us.
Regards,
Patsy and Sherri
Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC
IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045
P-720-859-4060
F-720-859-4110
email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net &
email <mailto:pruegg <@t> flagshipbio.com> pruegg <@t> flagshipbio.com
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
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------------------------------
Message: 2
Date: Mon, 23 Jul 2012 09:39:57 -0400
From: Eva Permaul <eca9 <@t> georgetown.edu>
Subject: [Histonet] Secondary antibody causing nuclear staining?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAEBNytUj-7CQ+nitodwtw018FHbJnT1StVgy1Rjn+ug7RH7VQA <@t> mail.gmail.com>
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Hello,
I have noticed that our biotinylated secondary antibodies on occasion cause
nuclear staining in some samples. Why is this? It is not every time so I
find it rather stange. Anyone know why this is happening and what I can do
to avoid it?
Thank you for any suggestion,
Eva Permaul
Georgetown University
------------------------------
Message: 3
Date: Mon, 23 Jul 2012 07:30:23 -0700
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Subject: RE: [Histonet] disposable blade holder
To: "'Patsy Ruegg'" <pruegg <@t> ihctech.net>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Cc: 'Sherri Saturley' <ssaturley <@t> flagshipbio.com>
Message-ID:
<493CAA64F203E14E8823737B9EE0E25F092BA8FC5B <@t> EXCHMB01.isis.local>
Content-Type: text/plain; charset="us-ascii"
Check with your local Leica rep.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Sunday, July 22, 2012 11:33 AM
To: Histonet <@t> lists.utsouthwestern.edu
Cc: 'Sherri Saturley'
Subject: [Histonet] disposable blade holder
We are looking for the disposable blade holder for low profile blades that fit in microtomes like the Leica 1510 where permanent blades used to be held. We have two of these but they are both for high profile blades and we would prefer to use low profile disposable blades. Does anyone have one of these sitting around used we could buy from you or if there are vendors selling used ones please feel free to contact us.
Regards,
Patsy and Sherri
Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC
IHCtech a subsidiary of Flagship Bio-Sciences, LLC Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045
P-720-859-4060
F-720-859-4110
email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net & email <mailto:pruegg <@t> flagshipbio.com> pruegg <@t> flagshipbio.com
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
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------------------------------
Message: 4
Date: Mon, 23 Jul 2012 08:57:17 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] disposable blade holder
To: "'MaryK Mendell'" <kmendell <@t> goldbergmd.net>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <64988B34442D48748488F3099B1D0D16 <@t> Patsyoffice>
Content-Type: text/plain; charset="us-ascii"
Yes it fits on the microtome where the permanent blades go for older
microtomes that do not have disposable blade holding stages.
Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC
IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045
P-720-859-4060
F-720-859-4110
email pruegg <@t> ihctech.net &
email pruegg <@t> flagshipbio.com
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
-----Original Message-----
From: MaryK Mendell [mailto:kmendell <@t> goldbergmd.net]
Sent: Monday, July 23, 2012 6:26 AM
To: Patsy Ruegg
Subject: RE: [Histonet] disposable blade holder
Patsy
are you talking about on the microtome it self.
Kate Mendell
Histopathology/Lab Manager
HOWARD S. GOLDBERG, M.D., INC
990 Paradise Road
Swampscott, MA 01907
TEL: 781.595.0151
FAX: 781.592.6780
kmendell <@t> goldbergmd.net
www.cosmesticdermcenter.com
PRIVACY NOTICE: This e-mail message may contain confidential patient or
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________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
[pruegg <@t> ihctech.net]
Sent: Sunday, July 22, 2012 2:33 PM
To: Histonet <@t> lists.utsouthwestern.edu
Cc: 'Sherri Saturley'
Subject: [Histonet] disposable blade holder
We are looking for the disposable blade holder for low profile blades that
fit in microtomes like the Leica 1510 where permanent blades used to be
held. We have two of these but they are both for high profile blades and we
would prefer to use low profile disposable blades. Does anyone have one of
these sitting around used we could buy from you or if there are vendors
selling used ones please feel free to contact us.
Regards,
Patsy and Sherri
Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC
IHCtech a subsidiary of Flagship Bio-Sciences, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045
P-720-859-4060
F-720-859-4110
email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net &
email <mailto:pruegg <@t> flagshipbio.com> pruegg <@t> flagshipbio.com
web site <http://www.ihctech.net> www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 23 Jul 2012 15:00:19 +0000
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Negative Reagent Control
To: Richard Cartun <Rcartun <@t> harthosp.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AEE9D3B1BA593A49B23DA5A6A375654CED907A9B <@t> EX2010-02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
For the last couple of years, I've thought this was true, but I didn't have the guts to say so. Thank you, Richard for bringing the truth out and getting it accepted.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Podiatric Medicine
Miami Shores, FL
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, July 17, 2012 7:05 PM
To: Histonet
Subject: [Histonet] Negative Reagent Control
Most of you know that I have been advocating for the elimination of the "Negative Reagent Control" when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence:
"Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director."
I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to "loud applause". This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen!
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
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------------------------------
Message: 6
Date: Mon, 23 Jul 2012 09:11:56 -0700 (PDT)
From: Michele Carr <michelecarr10 <@t> yahoo.com>
Subject: [Histonet] counter stain for PAS for fungus
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1343059916.21147.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus. The fast green stain is simply not staining anymore no matter how long I leave it on the specimen. Do you have any suggestions on how to correct this or perhaps recommendations for a different counterstain. Our protocol is as follows:
Periodic Acid 7 min
quick rinse
Schiff solution 20min
wash for 10min
Fast green stain 1 min
Any help is appreciated.
Thanks in advance,
Michele Carr
Medical Laboratory Services
------------------------------
Message: 7
Date: Mon, 23 Jul 2012 09:19:12 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] counter stain for PAS for fungus
To: Michele Carr <michelecarr10 <@t> yahoo.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1343060352.20905.YahooMailNeo <@t> web121406.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Prepare a fresh solution
René J.
________________________________
From: Michele Carr <michelecarr10 <@t> yahoo.com>
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, July 23, 2012 12:11 PM
Subject: [Histonet] counter stain for PAS for fungus
Hi everyone I am having a problem with the fast green counterstain for the PAS for fungus. The fast green stain is simply not staining anymore no matter how long I leave it on the specimen. Do you have any suggestions on how to correct this or perhaps recommendations for a different counterstain. Our protocol is as follows:
Periodic Acid 7 min
quick rinse
Schiff solution 20min
wash for 10min
Fast green stain 1 min
Any help is appreciated.
Thanks in advance,
Michele Carr
Medical Laboratory Services
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 23 Jul 2012 12:51:05 -0400
From: Rena Fail <renafail2 <@t> gmail.com>
Subject: Re: [Histonet] counter stain for PAS for fungus
To: Michele Carr <michelecarr10 <@t> yahoo.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAE7Zv_rPt1JCfWz3gw8Rhaukd2u_9YU9uCsAuJv5=njKyCN6Cw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I have used a working solution light green Sf yellowish C,I. # 42095 and
the only time I had problems was when someone left out the glacial acetic
acid in the stock,
Stock is light green SF yellowish 0.2 gm,distilled water 100.0 ml, 0.2 ml
glacial acetic acid.
Working is 10.0 ml of stock, 50.0 ml distilled water
Rena Fail
On Mon, Jul 23, 2012 at 12:11 PM, Michele Carr <michelecarr10 <@t> yahoo.com>wrote:
> Hi everyone I am having a problem with the fast green counterstain for the
> PAS for fungus. The fast green stain is simply not staining anymore no
> matter how long I leave it on the specimen. Do you have any suggestions on
> how to correct this or perhaps recommendations for a different
> counterstain. Our protocol is as follows:
> Periodic Acid 7 min
> quick rinse
> Schiff solution 20min
> wash for 10min
> Fast green stain 1 min
> Any help is appreciated.
> Thanks in advance,
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
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