[Histonet] Secondary antibody causing nuclear staining?

[Eva Permaul]

We believe it has to be the secondary as we have nuclear staining in the
absence of primary antibody.
Eva

On Mon, Jul 23, 2012 at 12:58 PM, ihcs-wojcieszyn <ihcs <@t> smithsys.net> wrote:

> Hello,
>         Please explain how you know that it is the secondary and not the
> primary? We Have confirmed that in canine lymphomas, nuclear
> CD79a is a real and frequent observation.
>
> john
> IHC Services
>
> On Monday, July 23, 2012, at 08:39 AM, Eva Permaul wrote:
>
>  Hello,
>>
>> I have noticed that our biotinylated secondary antibodies on occasion
>> cause
>> nuclear staining in some samples. Why is this? It is not every time so I
>> find it rather stange. Anyone know why this is happening and what I can do
>> to avoid it?
>>
>> Thank you for any suggestion,
>> Eva Permaul
>> Georgetown University
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>>
>