[Histonet] Golgi-Cox Sections

Amos Brooks amosbrooks <@t> gmail.com
Tue Jul 17 19:48:10 CDT 2012


Hi,
    For those that nave had the joy of performing the Golgi-Cox stain, you
will likely recall that it generally calls for some thick sections. The
researcher I am working with is hoping for 100 uM sections. That is way too
thick for a microtome and a cryostat. I suggested a vibratome might work
better, but not having used one I thought I should ask some folks that may
have encountered this. I know 100 uM is *really* thick, but he *really*
wants to follow the nerve tracks.

Thanks,
Amos


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