[Histonet] RE: Re: Methanol in H2O2 explanation

[Bea DeBrosse-Serra]

And the reason for that is.......???

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Wednesday, July 11, 2012 7:50 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Re: Methanol in H2O2 explanation

I made a mistake....I DO rehydrate before staining, as Tony pointed out.
I just don't use graded alcohols between the xylene and water ( x4  changes 74OP IMS).



Carl Hobbs
Histology Manager
Wolfson Centre for Age-Related Diseases
School of Biomedical Sciences
King's College London
Guys Campus
London SE1 1UL

Tel.020 7848 6810
fax 020 7848 6816
carl.hobbs <@t> kcl.ac.uk<mailto:carl.hobbs <@t> kcl.ac.uk>