[Histonet] RE: Re: Methanol in H2O2 explanation

[Settembre, Dana]

Carl,
H202 mixed with Buffer here.(instead of aq.)
That's OK too, right?
Dana

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Tuesday, July 10, 2012 11:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Methanol in H2O2 explanation

Why do some people use methanolic H2O2?
Why do people rehydrate after dewaxing?
Both are unnecessary, under usual conditions.

Methanol was used in the early days as a peroxidase blocker by itself.
The combination was devised as a "belt and bracer" method.
As you stated, you use aq H2O2 effectively.
So do I and many others.

However, for unfixed frozen sections, I would never use aq H2O2, if I wanted sections remaining on my slides....

After dewaxing, rinse sections in 4x 100% alcohol, rinse in water, endog. Px block while you make up you AR solutions....

Carl Hobbs
Histology and Imaging Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813


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