[Histonet] microscope ocular questions

[Morken, Timothy]

Thanks Peggy, 

That is clear. I used microscopes for years with one fixed ocular and one focusable ocular. I was wondering about why now both oculars are "focusable" yet one has more usability than the other. Maybe to accomodate greater variation? Or maybe is due to the advent of parfocal microscopes

 I found some instructions on parfocal adjustment that refers to setting both oculars to zero when doing the initial focus at high magnification, then setting the ocular adjustment for each eye at low magnification. So that makes sense for individualistic adjustment. However, I was asked why one ocular has easier use and more graduations that the other and I didn't have a good answer to that...The person thought the oculars were not the same so there was some problem with the microscope.

Tim

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
Sent: Tuesday, July 10, 2012 11:27 AM
To: Histonet
Subject: RE: [Histonet] microscope ocular questions


Tim, etal:

This is easily understood:  focusing and setup work the same on binoculars, one eyepiece is focused with the main focusing system, the other is used to match focusing with both eyes.  First focus the scope (binoculars or microscope) thru the simple (non focusing) eyepiece, then use the focusing eyepiece to fine tune focus for the other eye.  Once you've determined the setting on the focusing eyepiece, you can return the scope to this setting with ease and you should be able to use the scope for hours at a time without fatigue.

Each microscope or binoculars is different.  The setting for each person will be different (everybody's eyes are different).  Each of our eyes are different, thus the need for independent focusing for one eye.

Try defocusing the focusing eyepiece and using scope for a period.
Your eyes will have to work overtime to keep the image in focus (if you are young you might last longer than I would at 65) and you could get a headache or suffer fatigue.

Lee Wenk  (Peggy's husband)


-----Original Message-----
From: Morken, Timothy
Sent: Tuesday, July 10, 2012 1:39 PM
To: Histonet
Subject: [Histonet] microscope ocular questions

Histonet gurus,

Why is each microscope ocular marked and operated differently? For instance the right one has a knurled focusing ring,  is easily focused and has detailed graduations while the left one is not really set up to focus quickly and has only minimal graduations? Always wondered about this but can't find anything about it!

Thanks for your insights!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken <@t> ucsfmedctr.org


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