[Histonet] Methanol in H2O2 explanation

[Lewis, Patrick]

Hi everyone,

Can someone refresh/enlighten me as to why Methanol is used in H2O2 blocking.

Let's assume that I take my slides through the xylene/ethanol steps and that after the 70% etoh wash step, they are now in TBST.

I could see adding methanol if you were doing the H2O2 blocking as part of the xylene/etoh process and maybe you had the methanol as a substitute for the 70% etoh.

My xylene/ETOH looks like this.

3 x 5 min xylene.
2 x 5 min 100% etoh
2 x 5 min 95% etoh
1 x 5 min 70%
Then my slides are washed and then epitope retrieved.

After epitope retrieval they are again washed and then I do my H2O2 block without methanol.

Thanks

Patrick.




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