[Histonet] Re: Frozens and antigen retrieval

[Tim Higgins]

Agree, only if the antigen has been bound by a fixative.

-----Original ________________________________
From: Daniela Bodemer <daniela.bodemer <@t> mcri.edu.au>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 4, 2012 7:02 AM
Subject: [Histonet] Frozens and antigen retrieval

Hi all,

A question from my student: antigen retrieval when using frozens for 
immunofluorescence -yes or no?

The protocol suggested by the antibody company lists the retrieval as an 
option. I am used to do retrieval on paraffin sections, but not on cryo 
sections.

Hit me with your opinions on this :-)

Thanks in advance,

Daniela

Sent from my iPad