[Histonet] PCNA sc-7907?

[Mehmet Fatih BOZKURT]

dilute antibody with  %1 BSA.. and use %1 BSA for serum blocking...

On Wed, Jul 4, 2012 at 10:59 PM, Mehmet Fatih BOZKURT <fbozkurt <@t> gmail.com>wrote:

> dilute antibody with  %1 BSA.. and use %1 BSA for serum blocking...
>
> On Wed, Jul 4, 2012 at 10:21 PM, E Wolfe <ewolfe118 <@t> gmail.com> wrote:
>
>> Hello Histonet -
>>
>> Has anyone had any success staining with PCNA sc-7907 on frozen,
>> paraformaldehyde-fixed tissue? I am experiencing a lot of background, and
>> so far diluting down to 1:5000 hasn't helped at all. Any advice would be
>> very much appreciated!
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>
>
>
> --
> Mehmet Fatih BOZKURT, DVM, PhD
> Afyon Kocatepe University
> Faculty of Veterinary Medicine
> Department of Pathology
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-173/237
>
>


-- 
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-173/237