[Histonet] Automated Special Stainers
Romundstad, Pamela K
pkromund <@t> gundluth.org
Tue Jul 3 14:59:36 CDT 2012
Hello,
I'm interested in opinions on what Histologist prefer in their automated special stain instruments. Is it primarily between DAKO and Ventana? Any imput would be appreciated.
Pamela Romundstad HT, QIHC
Gundersen Lutheran
________________________________________
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Sent: Tuesday, July 03, 2012 12:00 PM
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Subject: Histonet Digest, Vol 104, Issue 3
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Today's Topics:
1. Embedding beads (Amber McKenzie)
2. Re: Embedding beads (Jennifer Campbell)
3. Re: Embedding beads (Alan Taylor)
4. Slide Baskets???? (heather marlatt)
5. Humidity Check (Laurie <@t> blufrogpath.com)
6. Mohs (Rebecca a. Johnson)
7. What is this item called? (Adam Haberman)
8. Re: What is this item called? (Jennifer MacDonald)
9. RE: What is this item called? (Rittman, Barry R)
10. RE: Mohs (Harrison, Sandra C.)
11. RE: Humidity Check (Harrison, Sandra C.)
12. Competency check list (JMaslanka <@t> stpetes.org)
13. RE: Humidity Check (Ingles Claire )
14. RE: Mohs (Ingles Claire )
15. JB fixative (Joe Hardin)
16. Re: Mohs (Kim Tournear)
17. Billing 88342 (Victor A. Tobias)
----------------------------------------------------------------------
Message: 1
Date: Mon, 2 Jul 2012 17:17:20 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] Embedding beads
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC308296E3 <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="iso-8859-1"
How do you not cut thru the bead when sectioning? I'm intrigued by this process b/c I've never heard of this before.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kelly Boyd
Sent: Tuesday, June 26, 2012 3:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Embedding beads
I started using the embedding beads several months ago as a quick way to track who embedded what cassette. Each tech is assigned a number according to the number on their microtome. I get the beads from Cancer Diagnostics. You can probably find something similar at a craft store.
These come in small round plastic containers that fit easily any where on the embedding center. They place the bead in a bottom corner of the cassette when topping it off with paraffin.
They?have actually saved us time. Once the techs get used to it, it might add a few seconds to the embedding.?Before, the techs had to write down which blocks they embedded. (Very time consuming and often not complete).?If I needed to see who embedded a certain block, I had to go check that log book. Now I can see who embedded it just by looking at the block.
Kelly??
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------------------------------
Message: 2
Date: Mon, 2 Jul 2012 14:29:33 -0400
From: Jennifer Campbell <campbellj <@t> muhlbauerlab.com>
Subject: Re: [Histonet] Embedding beads
To: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAEK9oWgV-Vy5Okp6QcZs4+r89SE4QN3b9jMW+-vrQqWn29dBEQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
The identifier(whatever is using to distinguish who did the task) is not
embedded with the specimen.
It is put in the top portion of the cassette.
At least that is what we have always done here.
On Mon, Jul 2, 2012 at 1:17 PM, Amber McKenzie <
amber.mckenzie <@t> gastrodocs.net> wrote:
> How do you not cut thru the bead when sectioning? I'm intrigued by this
> process b/c I've never heard of this before.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kelly Boyd
> Sent: Tuesday, June 26, 2012 3:20 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Embedding beads
>
> I started using the embedding beads several months ago as a quick way to
> track who embedded what cassette. Each tech is assigned a number according
> to the number on their microtome. I get the beads from Cancer Diagnostics.
> You can probably find something similar at a craft store.
> These come in small round plastic containers that fit easily any where on
> the embedding center. They place the bead in a bottom corner of the
> cassette when topping it off with paraffin.
> They have actually saved us time. Once the techs get used to it, it might
> add a few seconds to the embedding. Before, the techs had to write down
> which blocks they embedded. (Very time consuming and often not
> complete). If I needed to see who embedded a certain block, I had to go
> check that log book. Now I can see who embedded it just by looking at the
> block.
>
> Kelly
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
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>
--
Jen Campbell, HT(ASCP)
Supervisor of Technical Services
Muhlbauer Dermatopathology Laboratory
61 Monroe Avenue, Ste B
Pittsford NY 14534
P: 585.586.5166
F: 585.586.3137
IMPORTANT NOTICE: This e-mail and any attachments may contain confidential
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------------------------------
Message: 3
Date: Mon, 2 Jul 2012 19:37:50 +0100
From: "Alan Taylor" <aj.taylor <@t> blueyonder.co.uk>
Subject: Re: [Histonet] Embedding beads
To: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <3A078ACBD81E4F39B2B2C8FAD4C6332F <@t> merlin>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hi All
The embedding bead goes into the cassette tray, into one corner, not into
the stainless steel embedding mould with the tissue. The plastic cassette is
placed on top of the mould, as usual and filled with molten wax, the
coloured bead is quickly dropped in and the embedding mould is placed on the
chiller to solidify in preparation for microtomy. The bead has no contact
with the blade at any time, remaining at the back of the cassette tray.
Hope this clarifies this easy process.
Alan Taylor BSc(Hons), FRMS.
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. EX1 3AJ. Devon. UK.
----- Original Message -----
From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, July 02, 2012 6:17 PM
Subject: [Histonet] Embedding beads
How do you not cut thru the bead when sectioning? I'm intrigued by this
process b/c I've never heard of this before.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kelly Boyd
Sent: Tuesday, June 26, 2012 3:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Embedding beads
I started using the embedding beads several months ago as a quick way to
track who embedded what cassette. Each tech is assigned a number according
to the number on their microtome. I get the beads from Cancer Diagnostics.
You can probably find something similar at a craft store.
These come in small round plastic containers that fit easily any where on
the embedding center. They place the bead in a bottom corner of the cassette
when topping it off with paraffin.
They have actually saved us time. Once the techs get used to it, it might
add a few seconds to the embedding. Before, the techs had to write down
which blocks they embedded. (Very time consuming and often not complete). If
I needed to see who embedded a certain block, I had to go check that log
book. Now I can see who embedded it just by looking at the block.
Kelly
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 4
Date: Mon, 2 Jul 2012 12:17:01 -0700
From: heather marlatt <hmarlatt26 <@t> gmail.com>
Subject: [Histonet] Slide Baskets????
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CALaVVk7NYROyRSkgtFNCm-tLAv=n472pGU31B7stRD27k7H7ZA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I was wondering if anyone in histoland has any slide baskets for the old
sakura DRS 601 stainer laying around??? I can't seem to find these for a
decent price so I thought I'd ask. I only have one at the moment and as you
might imagine that us really cramping my style.
Thanks in advance
Heather
------------------------------
Message: 5
Date: Mon, 02 Jul 2012 13:19:43 -0700
From: <Laurie <@t> blufrogpath.com>
Subject: [Histonet] Humidity Check
To: "Histonet post" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<20120702131943.295dc6182df7e5cbb4f32bc101c30dcc.f604e52a69.wbe <@t> email15.secureserver.net>
Content-Type: text/plain; charset="utf-8"
Do others check the humidity of their Histo lab on a daily basis?
Laurie Colbert
------------------------------
Message: 6
Date: Mon, 2 Jul 2012 18:37:20 -0400
From: "Rebecca a. Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Mohs
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DD3B3698F51444EB9CA9CAF00DF92533 <@t> BeckyPC>
Content-Type: text/plain; charset="iso-8859-1"
Need to know what Mohs techs are getting paid.
Thanks
Becky
------------------------------
Message: 7
Date: Tue, 3 Jul 2012 08:45:53 -0400
From: Adam Haberman <adam.haberman <@t> oberlin.edu>
Subject: [Histonet] What is this item called?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CALdyYFqDQ87dVkbTcgtfAAHjREgyc41DpwRS232o1kCtcTyhfA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello everyone,
I am trying to identify a piece of glassware so that I can order a few more
of it. It is a thick piece of glass with a large, wide depression in the
middle. The item I am looking for is thicker and wider than a depression
slide, and I use it as a dissecting dish. I have included a picture of the
piece I am trying to identify, along with a depression slide for
comparison. This is probably an item that is not widely used anymore, as
it seems to predate all of the faculty here. However, it is perfect for
the dissections I do with two pair of forceps under a dissecting
microscope. It gives me much better working angles than the spot plates I
resort to when I cannot find this item.
If anyone can identify the item or, even better, tell me where I can order
more, please let me know. Thank you very much.
--Adam
Adam Haberman
Visiting Assistant Professor of Biology
Oberlin College
adam.haberman <@t> oberlin.edu
(440)775-6502
------------------------------
Message: 8
Date: Tue, 3 Jul 2012 06:13:37 -0700
From: Jennifer MacDonald <jmacdonald <@t> mtsac.edu>
Subject: Re: [Histonet] What is this item called?
To: Adam Haberman <adam.haberman <@t> oberlin.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2FB72470-1EA9-4BF5-8B04-4D613C5F06EE <@t> mtsac.edu>
Content-Type: text/plain; charset=us-ascii
A watch glass?
On Jul 3, 2012, at 5:45 AM, Adam Haberman <adam.haberman <@t> oberlin.edu> wrote:
> Hello everyone,
>
> I am trying to identify a piece of glassware so that I can order a few more
> of it. It is a thick piece of glass with a large, wide depression in the
> middle. The item I am looking for is thicker and wider than a depression
> slide, and I use it as a dissecting dish. I have included a picture of the
> piece I am trying to identify, along with a depression slide for
> comparison. This is probably an item that is not widely used anymore, as
> it seems to predate all of the faculty here. However, it is perfect for
> the dissections I do with two pair of forceps under a dissecting
> microscope. It gives me much better working angles than the spot plates I
> resort to when I cannot find this item.
>
> If anyone can identify the item or, even better, tell me where I can order
> more, please let me know. Thank you very much.
>
> --Adam
>
> Adam Haberman
> Visiting Assistant Professor of Biology
> Oberlin College
> adam.haberman <@t> oberlin.edu
> (440)775-6502
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Tue, 3 Jul 2012 08:15:31 -0500
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] What is this item called?
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<12A4DAFC2FEBB84B8DED5F5E9201B4E9179EC272E4 <@t> UTHCMS1.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Is this a 3 by 1.25 inch glass slide approximately 3 mm thick with a 1 inch depression in the middle?
Barry
To: Adam Haberman
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] What is this item called?
A watch glass?
On Jul 3, 2012, at 5:45 AM, Adam Haberman <adam.haberman <@t> oberlin.edu> wrote:
> Hello everyone,
>
> I am trying to identify a piece of glassware so that I can order a few more
> of it. It is a thick piece of glass with a large, wide depression in the
> middle. The item I am looking for is thicker and wider than a depression
> slide, and I use it as a dissecting dish. I have included a picture of the
> piece I am trying to identify, along with a depression slide for
> comparison. This is probably an item that is not widely used anymore, as
> it seems to predate all of the faculty here. However, it is perfect for
> the dissections I do with two pair of forceps under a dissecting
> microscope. It gives me much better working angles than the spot plates I
> resort to when I cannot find this item.
>
> If anyone can identify the item or, even better, tell me where I can order
> more, please let me know. Thank you very much.
>
> --Adam
>
> Adam Haberman
> Visiting Assistant Professor of Biology
> Oberlin College
> adam.haberman <@t> oberlin.edu
> (440)775-6502
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 10
Date: Tue, 3 Jul 2012 08:22:50 -0500
From: "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov>
Subject: RE: [Histonet] Mohs
To: "Rebecca a. Johnson" <raj <@t> bluemarble.net>, "histonet"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DB425E28065DA14FAA75A282959620AF06522A5F <@t> VHAV23MSGA2.v23.med.va.gov>
Content-Type: text/plain; charset="us-ascii"
Depends ;-)
All kidding aside, there are factors that weigh in, such as geography,
availability, training, experience, speed, etc.
10 years ago, when I worked as a Mohs' tech in the Denver, Colorado
area, I received $25.00 per hour, plus benefits. I usually only worked
3 days per week, but on some rare occasions, worked till 9 p.m. trying
to get to clear margins.
I don't know what the current wages are.
Sandy Harrison, HTL
Histology Supervisor, Minneapolis VA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
a. Johnson
Sent: Monday, July 02, 2012 5:37 PM
To: histonet
Subject: [Histonet] Mohs
Need to know what Mohs techs are getting paid.
Thanks
Becky
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------------------------------
Message: 11
Date: Tue, 3 Jul 2012 08:23:52 -0500
From: "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov>
Subject: RE: [Histonet] Humidity Check
To: <Laurie <@t> blufrogpath.com>, "Histonet post"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DB425E28065DA14FAA75A282959620AF06522A61 <@t> VHAV23MSGA2.v23.med.va.gov>
Content-Type: text/plain; charset="utf-8"
Yes, we have a wireless temperature monitoring system, called Checkpoint, and each room has a sensor that monitors temp and humidity.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie <@t> blufrogpath.com
Sent: Monday, July 02, 2012 3:20 PM
To: Histonet post
Subject: [Histonet] Humidity Check
Do others check the humidity of their Histo lab on a daily basis?=
Laurie Colbert
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------------------------------
Message: 12
Date: Tue, 3 Jul 2012 08:03:52 -0600
From: JMaslanka <@t> stpetes.org
Subject: [Histonet] Competency check list
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFD757EA4B.159651C3-ON87257A30.004D1C85-87257A30.004D427B <@t> stpetes.org>
Content-Type: text/plain; charset="US-ASCII"
Would anyone be willing to share their IHC tech competency check list?
Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126
Give thanks for ALL things.....
------------------------------
Message: 13
Date: Tue, 3 Jul 2012 10:06:08 -0500
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Humidity Check
To: "Histonet post" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<064F1ACBAE8A78469AE2E41D533D87E505A866 <@t> UWHC-MAIL2.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
I think it is actually a requirement that temp and humidity are monitiored daily.
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Laurie <@t> blufrogpath.com
Sent: Mon 7/2/2012 3:19 PM
To: Histonet post
Subject: [Histonet] Humidity Check
Do others check the humidity of their Histo lab on a daily basis?=
Laurie Colbert
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------------------------------
Message: 14
Date: Tue, 3 Jul 2012 10:07:13 -0500
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Mohs
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<064F1ACBAE8A78469AE2E41D533D87E505A867 <@t> UWHC-MAIL2.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
I would love to know this too. I am getting about $23/hr but I have pretty good benefits. I'm at UWisc Madison Hospital.
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Rebecca a. Johnson
Sent: Mon 7/2/2012 5:37 PM
To: histonet
Subject: [Histonet] Mohs
Need to know what Mohs techs are getting paid.
Thanks
Becky
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Tue, 03 Jul 2012 10:51:16 -0500
From: Joe Hardin <hardin <@t> oncology.wisc.edu>
Subject: [Histonet] JB fixative
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4FF314F4.5020707 <@t> oncology.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Hi All,
I use a Zinc fixative called JB fixitive for tissues that I want to
stain for CD antigens(see attachment). Does anyone know if this fixative
kills all microbes and prions?
------------------------------
Message: 16
Date: Tue, 3 Jul 2012 10:59:53 -0500
From: Kim Tournear <kim.tournear <@t> yahoo.com>
Subject: Re: [Histonet] Mohs
To: Ingles Claire <CIngles <@t> uwhealth.org>
Cc: histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5C862879-5A6D-4D83-A093-DACEC3AF0ACA <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
You can get upwards of 30.00/hr in Arizona.
Sent from the iPhone of Kim Tournear.
On Jul 3, 2012, at 10:07 AM, "Ingles Claire " <CIngles <@t> uwhealth.org> wrote:
> I would love to know this too. I am getting about $23/hr but I have pretty good benefits. I'm at UWisc Madison Hospital.
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Rebecca a. Johnson
> Sent: Mon 7/2/2012 5:37 PM
> To: histonet
> Subject: [Histonet] Mohs
>
>
>
>
> Need to know what Mohs techs are getting paid.
>
>
> Thanks
> Becky
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Tue, 3 Jul 2012 16:43:05 +0000
From: "Victor A. Tobias" <vtobias <@t> uw.edu>
Subject: [Histonet] Billing 88342
To: "'HISTONET'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C6003BE0D913D849A1FC97B6AA5B0FD00103BFB5 <@t> uwit-mbx09.exchange.washington.edu>
Content-Type: text/plain; charset="us-ascii"
Looking for other opinions from those who do consult/referral work.
If a client sends in a request for a single antibody done on multiple blocks on a single specimen, do you bill the client for each tech component
? The client will do the interpretation.
What happens in the above scenario if the request is to bill the patient? Knowing you get reimbursed for one, do you eat the other charges are make the client select the one block?
We have run numbers on potential lost revenue and the number is significant.
Victor
Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB244
Seattle, WA 98104
vtobias <@t> u.washington.edu<mailto:vtobias <@t> u.washington.edu>
206-744-2735
206-744-8240 Fax
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