[Histonet] Immunofluorescence staining/minimizing background staining

[Kasai, Miki (NIH/NCI) [E]]

Hi,

We are performing some immunofluorescence staining on mouse lung tissue.  We
are getting some nice positive staining with some of our initial antibodies
(procollagen, cytokeratin).

We would like to minimize the amount of background staining we are getting.
We are titering our primary antibodies to find out optimal Ab concentration
as well as the secondary conjugate Ab with the fluorophore of interest.  We
use donkey serum for general blocking.

Any other suggestions?

Much appreciation,
Miki