[Histonet] Immunofluorescence staining/minimizing background
Kasai, Miki (NIH/NCI) [E]
kasaim <@t> mail.nih.gov
Mon Jan 30 15:50:18 CST 2012
We are performing some immunofluorescence staining on mouse lung tissue. We
are getting some nice positive staining with some of our initial antibodies
We would like to minimize the amount of background staining we are getting.
We are titering our primary antibodies to find out optimal Ab concentration
as well as the secondary conjugate Ab with the fluorophore of interest. We
use donkey serum for general blocking.
Any other suggestions?
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