[Histonet] Re: Histonet Digest, Vol 98, Issue 37
Madeleine Huey
madeleinehuey <@t> gmail.com
Sat Jan 28 01:02:33 CST 2012
To: Jennifer K. Sipes
Here's my suggestion;
1) Perfuse the mouse with 10% NFB (their legs & tail will move during
perfusion, then harden if well perfused)
2) Remove intestine out & post-fix again with formalin for overnight
3) Fill a 10cc syringe with formalin & flush everything out
4) process & cut
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Fri, Jan 27, 2012 at 8:10 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> 1. Re: Histonet Digest, Vol 98, Issue 36 (Jennifer Sipes)
> 2. RE: Histonet Digest, Vol 98, Issue 35 (Reynolds,Donna M)
> 3. Fwd: [Histonet] oct to paraffin (Emily Sours)
> 4. RE: Re: Histonet Digest, Vol 98, Issue 36 (Truscott, Tom)
> 5. RE: Interview Questions (Tony Henwood (SCHN))
> 6. RE: Oil red O versus Sudan 4 (Tony Henwood (SCHN))
> 7. Re: Re: Histonet Digest, Vol 98, Issue 36 (Kim Donadio)
> 8. Re: Interview Questions (Joe Nocito)
> 9. Precipitate (Matt Mincer)
> 10. RE: Precipitate (Weems, Joyce)
> 11. RE: [Histonet] (iskaliora <@t> bioacademy.gr)
> 12. 2 New Perm Histology Opportunities (Matt Ward)
> 13. RE: Precipitate (Morken, Timothy)
> 14. Re: Precipitate (Akemi Allison)
> 15. RE: Precipitate (Morken, Timothy)
> 16. Gloves for coverslipping? (Tim Wheelock)
> 17. Histotech opening Orange County California (Paula Lucas)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 26 Jan 2012 10:13:16 -0800 (PST)
> From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1327601596.45466.YahooMailNeo <@t> web125402.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi Histonetters!
>
> I have a question that I'm hoping you guys can help me with. I have a person who brings me mouse intestinal tract with fecal matter still in it. She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter. Does anyone have any suggestions as to how best to handle this?? It seems to be getting worse the further into the study she goes.
>
> Thank you in advance for any help that you give!! It is truly appreciated!
>
> Jennifer K. Sipes, ALAT
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross 933
> 720 Rutland Avenue
> Baltimore, MD 21205
> phone: 410-614-0131
> fax: 410-955-9677
> cell: 443-631-6361
> e-mail: jsipes1 <@t> jhmi.edu
>
> ------------------------------
>
> Message: 2
> Date: Thu, 26 Jan 2012 12:17:53 -0600
> From: "Reynolds,Donna M" <dreynold <@t> mdanderson.org>
> Subject: [Histonet] RE: Histonet Digest, Vol 98, Issue 35
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <785BBF0C5F49CE41BA74460A43A08F023049632196 <@t> DCPWVMBXC0VS3.mdanderson.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
>
> I can definitely see some of the pitfallls to having someone cut. But it would certainly weed out anyone who didn't even know how to put a block in the microtome. This is also what probation periods are for to see if this person fits with your lab.
> But have you ever heard of a secretary hired without a typing test or 9 key if that was a heavy part of their job. Even with computers today this is still done.
> Donna
> Houston, TX
>
>
> Message: 3
> Date: Thu, 26 Jan 2012 02:59:49 +0000 (UTC)
> From: koellingr <@t> comcast.net
> Subject: Re: [Histonet] Interview Questions
> To: joelle weaver <joelleweaver <@t> hotmail.com>
> Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1983057611.84976.1327546789860.JavaMail.root <@t> sz0001a.emeryville.ca.mail.comcast.net>
>
> Content-Type: text/plain; charset=utf-8
>
> This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say "I object"? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview?
>
>
> Ray
> Seattle, WA
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 26 Jan 2012 14:24:46 -0500
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Subject: Fwd: [Histonet] oct to paraffin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAP=XX1z1_UR98BCq5-e79DS_ntWt2d+RSHca34O+RAdiiBBG_g <@t> mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> I may have missed this but does one fix the embryo again in Carnoy's or
> whatever after it has been washed (assuming it was fixed in the first place)
> I would think the initial para fixation would be enough and (again after
> washing in aqueous solution) dehydrating it up to xylene/toluene/non-aqeous
> solution before the paraffin steps.
> If one did fix again, would it be washed in 50% EtOH, 70% EtOH, before the
> fix?
> Or is this what the NBF washes are for? I have never used NBF, except when
> I tried to make Serra's with it, and yeah, that's not a good idea. Always
> use formalin, not NBF in Serra's or you'll get a lovely milky solution
> that's not fit for fixing.
>
> Emily
>
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
>
>
>
> On Wed, Jan 25, 2012 at 4:11 PM, Joseph Madary <patjnm <@t> gwumc.edu> wrote:
>
>> If you can spare some nbf the best thing to do would be to place the oct
>> block right into NBF and use that as the "wash" and then move onto a fresh
>> change of NBF. I would avoid straight water. NBF has enough water in it to
>> rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough
>> and a safe bet.
>>
>> Nick Madary, HT/HTL(ASCP)QIHC
>> George Washington University
>> Pathology Core Laboratory
>> Ross Hall, Room 706
>> 23rd and I Street NW
>> Washington D.C. 20037
>> 202.994.8196
>> patjnm <@t> gwumc.edu
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 26 Jan 2012 21:31:01 +0000
> From: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 98, Issue 36
> To: Jennifer Sipes <jengirl1014 <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9EF5279EBDFE6E4FB6605E8F183A002718833678 <@t> CVM76.vetmed.wsu.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Jennifer, I am by no means an expert on this, but have done a few mouse guts. I think that trying to flush out the feces with formalin or saline soon after necropsy, would help preserve the mucosa. The bacteria in the gut start breaking down the mucosa soon after death. Perhaps the gut is thin enough to fix rapidly enough to prevent damage to the mucosa without flushing. If that is the case, then flushing out the feces after fixation might help the quality of your slides. You may have to get permission to open up the gut to flush out the feces. It may hinge on how the tissue needs to be trimmed and oriented. Good luck, Tom Truscott
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes
> Sent: Thursday, January 26, 2012 10:13 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36
>
> Hi Histonetters!
>
> I have a question that I'm hoping you guys can help me with. I have a person who brings me mouse intestinal tract with fecal matter still in it. She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter. Does anyone have any suggestions as to how best to handle this?? It seems to be getting worse the further into the study she goes.
>
> Thank you in advance for any help that you give!! It is truly appreciated!
>
> Jennifer K. Sipes, ALAT
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross 933
> 720 Rutland Avenue
> Baltimore, MD 21205
> phone: 410-614-0131
> fax: 410-955-9677
> cell: 443-631-6361
> e-mail: jsipes1 <@t> jhmi.edu
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 26 Jan 2012 21:39:59 +0000
> From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
> Subject: RE: [Histonet] Interview Questions
> To: "'Joe Nocito'" <jnocito <@t> satx.rr.com>, joelle weaver
> <joelleweaver <@t> hotmail.com>, "trathborne <@t> somerset-healthcare.com"
> <trathborne <@t> somerset-healthcare.com>,
> "billodonnell <@t> catholichealth.net" <billodonnell <@t> catholichealth.net>,
> "sbreeden <@t> nmda.nmsu.edu" <sbreeden <@t> nmda.nmsu.edu>, Histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16CE9 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Joe,
>
> I would never wear a denim miniskirt!
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
> Sent: Thursday, 26 January 2012 11:14 AM
> To: joelle weaver; trathborne <@t> somerset-healthcare.com; billodonnell <@t> catholichealth.net; sbreeden <@t> nmda.nmsu.edu; Histonet
> Subject: Re: [Histonet] Interview Questions
>
> I used to give a 10 question test on general histology. I also had the expected answers written down and on my copy. Was accused once of being a racist. What saved me was having the answers in front of me. The person didn't get one answer correct. I had a couple of embedding questions, some cutting, special stains, immunos and some QC questions. I gave the interviewee the test while I was reviewing their resume. I would also see what their facial expressions were too. I had one person tell me they didn't do specials or immunos and didn't like embedding either. When I asked if they liked filing blocks and slides, they really would rather have a lab aide do it. This person didn't have to finish the test. Too make matters worse, she wore a denim miniskirt to boot. Just my three cents
>
> Joe
> ----- Original Message -----
> From: "joelle weaver" <joelleweaver <@t> hotmail.com>
> To: <trathborne <@t> somerset-healthcare.com>; <billodonnell <@t> catholichealth.net>;
> <sbreeden <@t> nmda.nmsu.edu>; "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, January 25, 2012 12:02 PM
> Subject: RE: [Histonet] Interview Questions
>
>
>
> Love this! I always want to do demonstration during technical interviews, but usually get "shot down" from managers and argued with in general, as in people don't feel that they should have to "prove" they can do histology.
> This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to "prove" or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general.
>
> Joelle Weaver MAOM, (HTL) ASCP
>
> http://www.linkedin.com/in/joelleweaver
>
> > From: trathborne <@t> somerset-healthcare.com
>> To: billodonnell <@t> catholichealth.net; sbreeden <@t> nmda.nmsu.edu;
>> histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 25 Jan 2012 17:47:01 +0000
>> Subject: RE: [Histonet] Interview Questions
>> CC:
>>
>> If your replacement will be doing actual histology, will your
>> institution permit the applicant to embed and cut? Can you sit down at
>> a multi-head scope and review slides with them?
>> What will the person be responsible for? Do they have experience with
>> all of these tasks? What would they do in a crisis situation (you can
>> make up one yourself that would be plausible).
>> People who volunteer in their personal lives, may do the same at work.
>> Ask how they juggle their schedule though, if there is a lot going on
>> in their personal lives. Be careful with how you ask these questions
>> though. Your HR department should be able to give you guidance in how to phrase things.
>> Good luck.
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>> O'Donnell, Bill
>> Sent: Wednesday, January 25, 2012 12:19 PM
>> To: Breeden, Sara; histonet <@t> lists.utsouthwestern.edu
>> Subject: RE: [Histonet] Interview Questions
>>
>> It would seem that questions like "How do you feel about cannibalism?"
>> might also be out but might be far more helpful; than "phone" questions.
>>
>>
>> On the serious side, when I was much younger I hired a person who was
>> able to answer all the right "histo" questions and so I hired him. He
>> turned out to be a poser, who, shortly after I fired him showed up at
>> a local university with a lab coat that listed him as "Dr." He had
>> indeed worked in a histo lab, but as a lab assistant, and so the the
>> understanding of what a histologist does was well rehearsed. (BTW, it
>> topok me about two weeks to catch on, though the more experienced
>> techs in the department figured it out almost right away)
>>
>> To be fair, it was during a time in hiring history when HR departments
>> were not willing to give useful reference data and there were only a
>> handful of questions they would even ask when checking. None of them
>> were particularly useful or telling. For inistance, they would not ask
>> if the person was an histo tech, but would simply ask, did he indeed
>> work at your institution?
>>
>> The place where I worked required little or nothing for proof of
>> experience. There was no background check either.
>>
>> Today, however, reference checking is a lot easier and more reliable.
>>
>> I guess my point here is that a good reference check needs to be done
>> as well weeding them out by histo questions. I'm sure your HR folks
>> will do a fine job of this.
>>
>> Also, once you have determined that they actually have the skills, or
>> a realistic potential of gaining them, questions concerning dynamics
>> of interaction are appropriate, though may lead to wrong impressions
>> in the mind of the applicant.
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>> Breeden, Sara
>> Sent: Wednesday, January 25, 2012 10:52 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Interview Questions
>>
>> So far, I am TOTALLY impressed and so grateful for your suggestions.
>> And here's why... did I ever tell anyone out there what the FIRST
>> question I was asked by the pathologist at my interview? It was.....
>> (wait for it....)
>>
>>
>>
>> "How do you feel about personal phone calls?". Un-freakin' believable.
>> I sure don't want someone to remember ME that way!!!
>>
>>
>>
>> Sally Breeden, HT(ASCP)
>>
>> New Mexico Department of Agriculture
>>
>> Veterinary Diagnostic Services
>>
>> 1101 Camino de Salud NE
>>
>> Albuquerque, NM 87102
>>
>> 505-383-9278 (Histology Lab)
>>
>>
>>
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> ------------------------------
>
> Message: 6
> Date: Thu, 26 Jan 2012 21:48:08 +0000
> From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
> Subject: RE: [Histonet] Oil red O versus Sudan 4
> To: "'Candice Smoots'" <candice_camille <@t> yahoo.com>, Histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A715760A16D55 <@t> xmdb02.nch.kids>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Candice,
>
> Remember freshly prepared Oil Red O Stock usually does not work.
> Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution.
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Candice Smoots
> Sent: Thursday, 26 January 2012 3:21 AM
> To: Histonet
> Subject: [Histonet] Oil red O versus Sudan 4
>
> Hi Histonetters
>
>
> I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and then i stain the inside of the aorta for my plaques.
>
> This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o.
>
> My question is what is the difference and what should I do differently with the Oil red o?
>
> Thanks so much for your help!
>
> I remain yours truely,
>
> Candice Camille
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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> This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
> *********************************************************************************
>
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 26 Jan 2012 16:48:41 -0500
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] Re: Histonet Digest, Vol 98, Issue 36
> To: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Jennifer Sipes
> <jengirl1014 <@t> yahoo.com>
> Message-ID: <A456638A-F783-4625-9AA3-01B475BFB964 <@t> yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Yep! That's sounds reasonable. Maybe you could use a syringe and flush the fecal material out with formalin? This way you shouldn't have to open it up. Best of luck!
> Kim :D
>
> Sent from my iPhone
>
> On Jan 26, 2012, at 4:31 PM, "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu> wrote:
>
>> Hi Jennifer, I am by no means an expert on this, but have done a few mouse guts. I think that trying to flush out the feces with formalin or saline soon after necropsy, would help preserve the mucosa. The bacteria in the gut start breaking down the mucosa soon after death. Perhaps the gut is thin enough to fix rapidly enough to prevent damage to the mucosa without flushing. If that is the case, then flushing out the feces after fixation might help the quality of your slides. You may have to get permission to open up the gut to flush out the feces. It may hinge on how the tissue needs to be trimmed and oriented. Good luck, Tom Truscott
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes
>> Sent: Thursday, January 26, 2012 10:13 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 36
>>
>> Hi Histonetters!
>>
>> I have a question that I'm hoping you guys can help me with. I have a person who brings me mouse intestinal tract with fecal matter still in it. She can NOT remove the fecal matter before processing due to the fact that her lab is studying the mucus membrane. She had asked the lab that was doing this how they got such prestine sections while we are having a hard time with the sections staying neat due to all the nicks caused by the fecal matter. Does anyone have any suggestions as to how best to handle this?? It seems to be getting worse the further into the study she goes.
>>
>> Thank you in advance for any help that you give!! It is truly appreciated!
>>
>> Jennifer K. Sipes, ALAT
>> Sr. Laboratory Technician
>> Johns Hopkins University
>> Ross 933
>> 720 Rutland Avenue
>> Baltimore, MD 21205
>> phone: 410-614-0131
>> fax: 410-955-9677
>> cell: 443-631-6361
>> e-mail: jsipes1 <@t> jhmi.edu
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 26 Jan 2012 17:01:04 -0600
> From: "Joe Nocito" <jnocito <@t> satx.rr.com>
> Subject: Re: [Histonet] Interview Questions
> To: "Tony Henwood \(SCHN\)" <tony.henwood <@t> health.nsw.gov.au>, "joelle
> weaver" <joelleweaver <@t> hotmail.com>,
> <trathborne <@t> somerset-healthcare.com>,
> <billodonnell <@t> catholichealth.net>, <sbreeden <@t> nmda.nmsu.edu>,
> "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E65ED4376C9E4DC59955A8E0204D49E4 <@t> JoePC>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> I would appreciate that Tony
> ----- Original Message -----
> From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
> To: "'Joe Nocito'" <jnocito <@t> satx.rr.com>; "joelle weaver"
> <joelleweaver <@t> hotmail.com>; <trathborne <@t> somerset-healthcare.com>;
> <billodonnell <@t> catholichealth.net>; <sbreeden <@t> nmda.nmsu.edu>; "Histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, January 26, 2012 3:39 PM
> Subject: RE: [Histonet] Interview Questions
>
>
> Joe,
>
> I would never wear a denim miniskirt!
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
> Sent: Thursday, 26 January 2012 11:14 AM
> To: joelle weaver; trathborne <@t> somerset-healthcare.com;
> billodonnell <@t> catholichealth.net; sbreeden <@t> nmda.nmsu.edu; Histonet
> Subject: Re: [Histonet] Interview Questions
>
> I used to give a 10 question test on general histology. I also had the
> expected answers written down and on my copy. Was accused once of being a
> racist. What saved me was having the answers in front of me. The person
> didn't get one answer correct. I had a couple of embedding questions, some
> cutting, special stains, immunos and some QC questions. I gave the
> interviewee the test while I was reviewing their resume. I would also see
> what their facial expressions were too. I had one person tell me they didn't
> do specials or immunos and didn't like embedding either. When I asked if
> they liked filing blocks and slides, they really would rather have a lab
> aide do it. This person didn't have to finish the test. Too make matters
> worse, she wore a denim miniskirt to boot. Just my three cents
>
> Joe
> ----- Original Message -----
> From: "joelle weaver" <joelleweaver <@t> hotmail.com>
> To: <trathborne <@t> somerset-healthcare.com>; <billodonnell <@t> catholichealth.net>;
> <sbreeden <@t> nmda.nmsu.edu>; "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, January 25, 2012 12:02 PM
> Subject: RE: [Histonet] Interview Questions
>
>
>
> Love this! I always want to do demonstration during technical interviews,
> but usually get "shot down" from managers and argued with in general, as in
> people don't feel that they should have to "prove" they can do histology.
> This perception, I never got, because I always saw it as in a job
> interview-in what other situation are you more trying to "prove" or impress
> with your knowledge, attitude, skills and experience? If you do bench work,
> you can tell in just a few minutes of observation much more information than
> you could get with quite a few questions. To be fair, I take into account
> nervousness, being closely observed, and lack of familiarity with equipment
> etc. I don't know, I think its fair if those are important skills to the
> position/role. Was not sure if Sara's job was mostly technical though, so
> thought I might keep it general.
>
> Joelle Weaver MAOM, (HTL) ASCP
>
> http://www.linkedin.com/in/joelleweaver
>
> > From: trathborne <@t> somerset-healthcare.com
>> To: billodonnell <@t> catholichealth.net; sbreeden <@t> nmda.nmsu.edu;
>> histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 25 Jan 2012 17:47:01 +0000
>> Subject: RE: [Histonet] Interview Questions
>> CC:
>>
>> If your replacement will be doing actual histology, will your
>> institution permit the applicant to embed and cut? Can you sit down at
>> a multi-head scope and review slides with them?
>> What will the person be responsible for? Do they have experience with
>> all of these tasks? What would they do in a crisis situation (you can
>> make up one yourself that would be plausible).
>> People who volunteer in their personal lives, may do the same at work.
>> Ask how they juggle their schedule though, if there is a lot going on
>> in their personal lives. Be careful with how you ask these questions
>> though. Your HR department should be able to give you guidance in how to
>> phrase things.
>> Good luck.
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>> O'Donnell, Bill
>> Sent: Wednesday, January 25, 2012 12:19 PM
>> To: Breeden, Sara; histonet <@t> lists.utsouthwestern.edu
>> Subject: RE: [Histonet] Interview Questions
>>
>> It would seem that questions like "How do you feel about cannibalism?"
>> might also be out but might be far more helpful; than "phone" questions.
>>
>>
>> On the serious side, when I was much younger I hired a person who was
>> able to answer all the right "histo" questions and so I hired him. He
>> turned out to be a poser, who, shortly after I fired him showed up at
>> a local university with a lab coat that listed him as "Dr." He had
>> indeed worked in a histo lab, but as a lab assistant, and so the the
>> understanding of what a histologist does was well rehearsed. (BTW, it
>> topok me about two weeks to catch on, though the more experienced
>> techs in the department figured it out almost right away)
>>
>> To be fair, it was during a time in hiring history when HR departments
>> were not willing to give useful reference data and there were only a
>> handful of questions they would even ask when checking. None of them
>> were particularly useful or telling. For inistance, they would not ask
>> if the person was an histo tech, but would simply ask, did he indeed
>> work at your institution?
>>
>> The place where I worked required little or nothing for proof of
>> experience. There was no background check either.
>>
>> Today, however, reference checking is a lot easier and more reliable.
>>
>> I guess my point here is that a good reference check needs to be done
>> as well weeding them out by histo questions. I'm sure your HR folks
>> will do a fine job of this.
>>
>> Also, once you have determined that they actually have the skills, or
>> a realistic potential of gaining them, questions concerning dynamics
>> of interaction are appropriate, though may lead to wrong impressions
>> in the mind of the applicant.
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>> Breeden, Sara
>> Sent: Wednesday, January 25, 2012 10:52 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Interview Questions
>>
>> So far, I am TOTALLY impressed and so grateful for your suggestions.
>> And here's why... did I ever tell anyone out there what the FIRST
>> question I was asked by the pathologist at my interview? It was.....
>> (wait for it....)
>>
>>
>>
>> "How do you feel about personal phone calls?". Un-freakin' believable.
>> I sure don't want someone to remember ME that way!!!
>>
>>
>>
>> Sally Breeden, HT(ASCP)
>>
>> New Mexico Department of Agriculture
>>
>> Veterinary Diagnostic Services
>>
>> 1101 Camino de Salud NE
>>
>> Albuquerque, NM 87102
>>
>> 505-383-9278 (Histology Lab)
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> This electronic mail and any attached documents are intended solely
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>> that reading, copying, or disclosing this email is prohibited. If you
>> received this email in error, immediately reply to the sender and
>> delete the message completely from your computer system.
>>
>> _______________________________________________
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>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> CONFIDENTIALITY NOTICE
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>> Center and are intended only for the addressee. The information
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>> Unauthorized forwarding, printing, copying, distribution, or use of
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>> are not the addressee, please promptly delete this message and notify
>> the sender of the delivery error by e-mail or you may call Somerset
>> Medical Center's computer Help Desk at 908-685-2200, ext. 4050.
>>
>> Be sure to visit Somerset Medical Center's Web site -
>> www.somersetmedicalcenter.com - for the most up-to-date news, event
>> listings, health information and more.
>>
>> _______________________________________________
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>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************************
> This email and any files transmitted with it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed.
> If you are not the intended recipient, please delete it and notify the
> sender.
>
> Views expressed in this message and any attachments are those of the
> individual sender, and are not necessarily the views of The Children's
> Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned and
> although no computer viruses were detected, The Childrens Hospital at
> Westmead accepts no liability for any consequential damage resulting from
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> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 26 Jan 2012 19:57:19 -0600
> From: Matt Mincer <techonebs <@t> comcast.net>
> Subject: [Histonet] Precipitate
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4F22047F.9010400 <@t> comcast.net>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hey Histonet,
>
> We have a client who is having an odd problem with their processor. They
> are getting "sandy" clogs in station 3. The original thought was that it
> was formalin salts but the texture and color was wrong. Also, station 3
> is 70% which should be weak enough. One of the techs mentioned in
> passing that the water quality in their town was really bad. I think
> that the problem is that, like formalin, the alcohol is causing
> dissolved minerals to be released from the tap water they use to mix
> their 70%. Has anyone seen this before or am I chasing a harebrained
> theory? Any thoughts would be greatly appreciated.
>
> Thanks
> Matt
>
> --
> Matthew Mincer
> Tech One Biomedical Services
> 159 N Marion Street, PMB163
> Oak Park, IL 60301
> (708) 383-6040 X 10
> fax (708) 383-6045
> cell (708) 822-3738
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 27 Jan 2012 00:35:29 -0500
> From: "Weems, Joyce" <JWeems <@t> sjha.org>
> Subject: RE: [Histonet] Precipitate
> To: "matt <@t> techonebiomedical.com" <matt <@t> techonebiomedical.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <92AD9B20A6C38C4587A9FEBE3A30E1640846C1E8D9 <@t> CHEXCMS10.one.ads.che.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Could they use DI water to test that theory?
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matt Mincer
> Sent: Thursday, January 26, 2012 20:57
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Precipitate
>
> Hey Histonet,
>
> We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated.
>
> Thanks
> Matt
>
> --
> Matthew Mincer
> Tech One Biomedical Services
> 159 N Marion Street, PMB163
> Oak Park, IL 60301
> (708) 383-6040 X 10
> fax (708) 383-6045
> cell (708) 822-3738
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Confidentiality Notice:
> This e-mail, including any attachments is the
> property of Catholic Health East and is intended
> for the sole use of the intended recipient(s).
> It may contain information that is privileged and
> confidential. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are
> not the intended recipient, please delete this message, and
> reply to the sender regarding the error in a separate email.
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Fri, 27 Jan 2012 09:22:00 +0200
> From: iskaliora <@t> bioacademy.gr
> Subject: RE: [Histonet]
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <d8f45e6d02f6a3e333590b95b72f0c79.squirrel <@t> webmail.bioacademy.gr>
> Content-Type: text/plain;charset=iso-8859-7
>
> please remove my name from the list.
> many thanks in advance,
> I. Skaliora
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 27 Jan 2012 08:15:44 -0500
> From: Matt Ward <mw <@t> personifysearch.com>
> Subject: [Histonet] 2 New Perm Histology Opportunities
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <1a817c77dcb05a27c3c4341e4a80c053 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Good Morning Histonet,
>
>
>
> Personify has had 2 new permanent Histology opportunities become available
> in the Northern IL area.
>
>
>
> Please contact me directly at mw <@t> personifysearch to learn more.
>
>
>
> *The Company:*
>
> Well-established provider of consumables and medical device accessories for
> clinical histology and research laboratories. The facility works closely
> with our UK, German and Australian facilities in the development,
> manufacture and marketing of products including processing reagents,
> storage and specimen transport devices, cytology accessories and safety
> products.
>
> This is a globally focused business with significant sales and operations
> in the US, Europe and Asia Pacific as well as a direct presence in over 100
> countries.
>
> *The Opportunities:*
>
> (1) QA Histologist: The company currently has an opening for a Quality
> Assurance Histologist to be based in Richmond IL.
>
> (2) Histologist: The company currently has an opening for a Histologist
> to be based in Richmond IL.
>
> Salary: Based on Experience
>
> Other: Full benefits - 401k program/matching
>
>
> *Education and Experience Required:*
>
> - Experience with tissue grossing, tissue processing and embedding
> - Experience with sectioning paraffin embedded tissue as well as frozen
> tissue
> - Experience performing routine stains (H and E) as well as special stains
> - Experience with formulation and production of routine laboratory reagents
> and solutions
> - Experience performing and documenting routine laboratory procedures
> - Familiarity with compliance requirements in the medical device industry
> - Proficiency in basic computer skills and with software applications such
> as Microsoft Office
>
> Have a great day!
>
>
>
> Regards,
>
>
>
>
>
> Matt Ward
>
> *Account Executive*
>
> *Personify*
>
> 5020 Weston Parkway Suite 315
>
> Cary NC 27513
>
> (Tel) 800.875.6188 direct ext 103
>
> (Fax) 919.460.0642
>
> www.personifysearch.com
>
>
> ------------------------------
>
> Message: 13
> Date: Fri, 27 Jan 2012 06:23:50 -0800
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: RE: [Histonet] Precipitate
> To: "'matt <@t> techonebiomedical.com'" <matt <@t> techonebiomedical.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <8D7C2D242DBD45498006B21122072BF89F5EE71E <@t> MCINFRWEM003.ucsfmedicalcenter.org>
>
> Content-Type: text/plain; charset=us-ascii
>
> Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse of the first 4 stations. It is the oldest processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to "get to the bottom of it" and he found the steel tubing clogged with "sand." It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes.
>
> Since then, no problems at all.
>
> Tim Morken
> Supervisor, Histology, IPOX
> UCSF Medical Center
> San Francisco, CA, USA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matt Mincer
> Sent: Thursday, January 26, 2012 5:57 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Precipitate
>
> Hey Histonet,
>
> We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated.
>
> Thanks
> Matt
>
> --
> Matthew Mincer
> Tech One Biomedical Services
> 159 N Marion Street, PMB163
> Oak Park, IL 60301
> (708) 383-6040 X 10
> fax (708) 383-6045
> cell (708) 822-3738
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 27 Jan 2012 06:39:18 -0800
> From: Akemi Allison <akemiat3377 <@t> yahoo.com>
> Subject: Re: [Histonet] Precipitate
> To: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, "'matt <@t> techonebiomedical.com'"
> <matt <@t> techonebiomedical.com>
> Message-ID: <44F7D4B6-DDBF-4E82-8C42-060A4583EC4D <@t> yahoo.com>
> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
>
> I agree with Tim, but I am curious why tap water is being used to
> make the 70% alcohol instead of DI water. Tap water has been known
> to have all kinds of contaminates.
>
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com
>
> On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote:
>
>> Matt, we have seen that in one of our VIP5's and we do weekly hot-
>> water rinse of the first 4 stations. It is the oldest processor,
>> at about 7 years. That processor had frequent pump-in pump-out
>> errors randomly in the first 3 stations. Finally the service tech
>> decided to "get to the bottom of it" and he found the steel tubing
>> clogged with "sand." It took a whole day to clean it all out. It
>> did not look like formalin salts, but did look like the kind of
>> deposits that you see with hard water in pipes.
>>
>> Since then, no problems at all.
>>
>> Tim Morken
>> Supervisor, Histology, IPOX
>> UCSF Medical Center
>> San Francisco, CA, USA
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
>> bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matt Mincer
>> Sent: Thursday, January 26, 2012 5:57 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Precipitate
>>
>> Hey Histonet,
>>
>> We have a client who is having an odd problem with their processor.
>> They are getting "sandy" clogs in station 3. The original thought
>> was that it was formalin salts but the texture and color was wrong.
>> Also, station 3 is 70% which should be weak enough. One of the
>> techs mentioned in passing that the water quality in their town was
>> really bad. I think that the problem is that, like formalin, the
>> alcohol is causing dissolved minerals to be released from the tap
>> water they use to mix their 70%. Has anyone seen this before or am
>> I chasing a harebrained theory? Any thoughts would be greatly
>> appreciated.
>>
>> Thanks
>> Matt
>>
>> --
>> Matthew Mincer
>> Tech One Biomedical Services
>> 159 N Marion Street, PMB163
>> Oak Park, IL 60301
>> (708) 383-6040 X 10
>> fax (708) 383-6045
>> cell (708) 822-3738
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 27 Jan 2012 07:17:33 -0800
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: RE: [Histonet] Precipitate
> To: "'Akemi Allison'" <akemiat3377 <@t> yahoo.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, "'matt <@t> techonebiomedical.com'"
> <matt <@t> techonebiomedical.com>
> Message-ID:
> <8D7C2D242DBD45498006B21122072BF89F5EE720 <@t> MCINFRWEM003.ucsfmedicalcenter.org>
>
> Content-Type: text/plain; charset=us-ascii
>
> I was/am wondering because we do use Di water for our 80% (our lowest ETOH). But it that processor was used in a different lab for several years so maybe it got started there.
>
> None of our other 3 processors had the problem.
>
> Tim Morken
> Supervisor, Histology, IPOX
> UCSF Medical Center
> San Francisco, CA, USA
> From: Akemi Allison [mailto:akemiat3377 <@t> yahoo.com]
> Sent: Friday, January 27, 2012 6:39 AM
> To: Morken, Timothy
> Cc: 'matt <@t> techonebiomedical.com'; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Precipitate
>
> I agree with Tim, but I am curious why tap water is being used to make the 70% alcohol instead of DI water. Tap water has been known to have all kinds of contaminates.
>
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com<mailto:akemiat3377 <@t> yahoo.com>
>
> On Jan 27, 2012, at 6:23 AM, Morken, Timothy wrote:
>
>
> Matt, we have seen that in one of our VIP5's and we do weekly hot-water rinse of the first 4 stations. It is the oldest processor, at about 7 years. That processor had frequent pump-in pump-out errors randomly in the first 3 stations. Finally the service tech decided to "get to the bottom of it" and he found the steel tubing clogged with "sand." It took a whole day to clean it all out. It did not look like formalin salts, but did look like the kind of deposits that you see with hard water in pipes.
>
> Since then, no problems at all.
>
> Tim Morken
> Supervisor, Histology, IPOX
> UCSF Medical Center
> San Francisco, CA, USA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matt Mincer
> Sent: Thursday, January 26, 2012 5:57 PM
> To: histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
> Subject: [Histonet] Precipitate
>
> Hey Histonet,
>
> We have a client who is having an odd problem with their processor. They are getting "sandy" clogs in station 3. The original thought was that it was formalin salts but the texture and color was wrong. Also, station 3 is 70% which should be weak enough. One of the techs mentioned in passing that the water quality in their town was really bad. I think that the problem is that, like formalin, the alcohol is causing dissolved minerals to be released from the tap water they use to mix their 70%. Has anyone seen this before or am I chasing a harebrained theory? Any thoughts would be greatly appreciated.
>
> Thanks
> Matt
>
> --
> Matthew Mincer
> Tech One Biomedical Services
> 159 N Marion Street, PMB163
> Oak Park, IL 60301
> (708) 383-6040 X 10
> fax (708) 383-6045
> cell (708) 822-3738
>
>
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>
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>
>
> ------------------------------
>
> Message: 16
> Date: Fri, 27 Jan 2012 10:48:05 -0500
> From: Tim Wheelock <twheelock <@t> mclean.harvard.edu>
> Subject: [Histonet] Gloves for coverslipping?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4F22C735.1060104 <@t> mclean.harvard.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hi Everybody:
>
> Does anyone know of a xylene-resistant glove that can be used for
> coverslipping, and that allows the dexterity necessary to coverslip?
>
> Thanks,
>
> Tim Wheelock
> Harvard Brain Bank
> McLean Hospital
> Belmont, MA
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Fri, 27 Jan 2012 08:10:27 -0800
> From: "Paula Lucas" <plucas <@t> biopath.org>
> Subject: [Histonet] Histotech opening Orange County California
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <03F37C569B91460393E1E27893BCD837 <@t> biopath.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We have a part time histotech position available working from 5 am on
> Tuesday through Saturdays. We are located in Fountain Valley, California.
>
> Please provide me a summary of your histotech experience and please send me
> your resume.
>
> Thank you,
>
> Paula Lucas
>
>
>
> ------------------------------
>
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> End of Histonet Digest, Vol 98, Issue 37
> ****************************************
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