[Histonet] Oil red O versus Sudan 4

[Tony Henwood (SCHN)]

Candice,

Remember freshly prepared Oil Red O Stock usually does not work.
Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Thursday, 26 January 2012 3:21 AM
To: Histonet
Subject: [Histonet] Oil red O versus Sudan 4

Hi Histonetters
 
 
I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain the inside of the aorta for my plaques.
 
This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o.
 
My question is what is the difference and what should I do differently with the Oil red o?
 
Thanks so much for your help!

I remain yours truely, 

Candice Camille 
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