[Histonet] Oil red O versus Sudan 4

Tony Henwood (SCHN) tony.henwood <@t> health.nsw.gov.au
Thu Jan 26 15:48:08 CST 2012


Remember freshly prepared Oil Red O Stock usually does not work.
Prepare your saturated Oil Red O Stock and leave it for a week or more before using it to prepare a working solution.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Thursday, 26 January 2012 3:21 AM
To: Histonet
Subject: [Histonet] Oil red O versus Sudan 4

Hi Histonetters
I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain the inside of the aorta for my plaques.
This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o.
My question is what is the difference and what should I do differently with the Oil red o?
Thanks so much for your help!

I remain yours truely, 

Candice Camille 
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list