[Histonet] neutrophil marker for FFPE mouse tissue

[Amos Brooks]

Hi,
    I agree with Liz Chlipala's suggestion for the Serotec antibody. It
works nicely. I recommend a protein block though. It does tend toward the
background. I use Proteinase K rather than HIER.

Amos

On Wed, Jan 25, 2012 at 11:22 AM, <histonet-request <@t> lists.utsouthwestern.edu
> wrote:

> Message: 20
> Date: Wed, 25 Jan 2012 08:11:14 -0800 (PST)
> From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
> Subject: [Histonet] neutrophil marker for FFPE mouse tissue
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <1327507874.8565.YahooMailNeo <@t> web130104.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Hello!
>
> What antibodies are people using to stain for neutrophils in FFPE mouse
> tissue?
>
> Kim
>
>
>
> Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA
>