[Histonet] Oil red O versus Sudan 4

[Candice Smoots]

Hi Sarah
 
 Actually, There is no micotmy involved. We biopsy the aorta. Clean the surrounding fat, cut it down the middle with a blade. And then we stain with Sudan 4. The aorta is mounted whole on a wax plate. we hydrate the tissue with PBS before we stain. This worked fine with the Sudan 4. Should I discontinue hydrating with PBS and use water instead with the Oil Red O.
 
Also with the Oil red O, we were rinsing them with isopranol before we stained them. Could this be the problem?

I remain yours truely, 

Candice Camille 


________________________________
From: Sarah Dysart <sdysart <@t> mirnarx.com>
To: Candice Smoots <candice_camille <@t> yahoo.com> 
Sent: Wednesday, January 25, 2012 10:32 AM
Subject: RE: [Histonet] Oil red O versus Sudan 4

I do oil red a lot.  You are doing it on frozen sections right?
What are you coverslipping with?
Need a few more details of the protocol and I might be able to help =)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Wednesday, January 25, 2012 10:21 AM
To: Histonet
Subject: [Histonet] Oil red O versus Sudan 4

Hi Histonetters
 
 
I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and  then i stain the inside of the aorta for my plaques.
 
This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o.
 
My question is what is the difference and what should I do differently with the Oil red o?
 
Thanks so much for your help!

I remain yours truely, 

Candice Camille 
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