[Histonet] Pearls Prussian blue with green counterstain

[Tony Henwood (SCHN)]

I would have thought that Perls with a fast green counterstain would lack contrast (green and blue?0

Rather than nuclear fast red (which I find very pretty) you could use 1% neutral red in 2% acetic acid , or 1% Bismark Brown in 1% acetic acid, or even 15sec in your H&E eosin solution.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Colleen Forster
Sent: Tuesday, 24 January 2012 4:45 AM
To: Histonet
Subject: [Histonet] Pearls Prussian blue with green counterstain

I am wondering if any of you have ever used a Fast Green counter stain in place of the Nuclear Fast Red? If so, what timing did you use?

Thanks,

Colleen Forster
U of MN

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