[Histonet] clostridium and bacterial spores in tissue sections

[Elizabeth Chlipala]

Great thanks, I think I'll just try a gram stain to start off with.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe
Sent: Monday, January 16, 2012 6:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: re: [Histonet] clostridium and bacterial spores in tissue sections

Hi Liz,
I'm not sure why a good old Gram (or Brown and Brenn) wouldn't work for your sections. I have visualized clostridia with such stains in the past, and the terminal spores were easily visible.

__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
Vice-President, Pathologie
AccelLAB Inc
1635 Lionel-Bertrand, Boisbriand
Québec, Canada  J7H 1N8
tel:  450-435-9482 ext.247
fax: 450-435-4795
jm.lapointe <@t> accellab.com



--- On Sat, 1/14/12, Elizabeth Chlipala <liz <@t> premierlab.com> wrote:


From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] clostridium and bacterial spores in tissue sections
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, January 14, 2012, 12:58 PM


Hello everyone

I need a bit of help.  I have to stain for clostridium and bacterial spores in tissue sections.  I have come across a few references to staining these on smears but not on tissue sections.  I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani.  Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections?  Any advice would be appreciated.

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com<http://www.premierlab.com>

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