[Histonet] RE: B-cell Ab to work on rat tissue

[koellingr <@t> comcast.net]

Carl's choice is excellent. The classical B-cell marker might be CD20, and does work great, but as with all antibodies, you have to be aware of exactly what you are after. CD20 comes on line after CD19 induction so CD20 might not be seen on very early b-cells. Like wise CD79a is accompanied by chaperone proteins prior to it's assemblage with the B cell receptor (during the pro-B stage). Won't mark these very early cells well. The assemblage is complete at pre-B stage and continues expression even till plasma cell stage. So true for almost all b cells, CD79a is great and you can see rat tissue IHC of CD79A out on the web, have used several of them, along with protocols (HIER as Carl pointed out works well). The complex is found on virtually no other cell as it is the signalling complex for the b cell receptor. 


Ray 
Ray Koelling 
PhenoPath Labs 
Seattle, WA 
----- Original Message -----
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk> 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Sunday, January 15, 2012 8:24:59 AM 
Subject: [Histonet] RE: B-cell Ab to work on rat tissue 


It may be that CD79a Ab is appropriate? 
It works well on Pwax sections, after HIER. 
I do not know the specificity of this Ag for B cells, so would be interested in feedback 

Check here for images : http://www.immunoportal.com/modules.php?name=gallery2 

Carl Hobbs 
Histology Manager 
Wolfson CARD 
School of Biomedical Sciences 
Kings College London 
Guys Campus 
SE1 1UL 
Tel: 020 78486813 
Fax: 020 78486816 
020 78486813 


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