[Histonet] Pms 2 antibody trouble

[Karen Lahti]

We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. 

 Karen Lahti, HT, QIHC
Arizona Digestive Health
602-687-7468


On Jan 12, 2012, at 9:42 AM, "Freeman, Carol" <Carol.Freeman <@t> utoledo.edu> wrote:

> 
> 
> I am curious if anyone is having trouble validating PMS2 antibody for
> the MSI panel.  We have worked this antbody up extensively and we are
> finding that it is on either side of the spectrum just too light (it is
> either light staining if any at all).   We have stained a few cases
> beautifully but nothing is consistent.  We are looking to try another
> vendor's antibodies, but were hoping for some feedback as to which labs
> have found success in this area.  I have never had trouble like this
> with an antibody.  The severe problem here is that with MSI testing, we
> are looking for lack of staining with this antibody to show a positive
> result so light patchy staining leaves a lot of room for error.  Any
> responses are appreciated.
> 
> Thank you,
> Carol E. Freeman HTL (ASCP) B.S.
> Department of Pathology
> University of Toledo Medical Center
> 3000 Arlington Avenue
> Toledo, OH 43614-5807
> carol.freeman <@t> utoledo.edu
> (419)383-5639
> 
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