[Histonet] CD marker help [particularly CD68]
TillRenee <@t> uams.edu
Tue Jan 10 10:21:54 CST 2012
Hello! I have been trying to get CD68 (CD11b and F4/80 as well, though I am focusing on the CD68 for now)on some mouse adipose tissue and have been getting varying results. I don't have any experience working with CD marker or doing IHC on adipose tissue. Without getting into the whole long process I have had trying to optimize this antibody, I am hoping someone can answer a couple of questions for me.
1. Can CD marker, and CD68 in particular, be picky as far as needing fresh cut FFPE slides? With everything I have tried, this is the only thing I can come up with for the inconsistencies I have experienced.
2. Positive control. I do not have one. Any suggestions? At least I do not have a positive control adipose block. I have tried mouse spleen, lung, and duo, and all have worked, but not consistently, and not in keeping with my adipose test slides. I can have beautiful stained spleen and duo, but my adipose slide will be completely brown. I believe I have about worked this issue out....as far as getting good staining on the fat and the control tissues, but then I encounter what led to my question #1.
Renee' Fox, HT (ASCP)
Arkansas Children's Nutrition Center
15 Children's Way./N2021
Little Rock, AR 72202
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