[Histonet] RE: Histonet Digest, Vol 98, Issue 11
Wellen, Terrence D. :LPH Lab
TWELLEN <@t> LHS.ORG
Mon Jan 9 12:10:51 CST 2012
ventana offers a basic course in tuscon.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, January 09, 2012 10:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 98, Issue 11
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re:ihc course (naveeda arshad)
2. Re: Re: Quality assurance program for pathologists (Kim Donadio)
3. Re: Re:ihc course (Kim Donadio)
4. Re: Re:ihc course (Lee & Peggy Wenk)
5. Stability of Retic stain in Bone Marrow (Elizabeth Chatfield)
6. Respirators and Routine Histology (Genest, Sharon SktnHR)
7. Electron Microscopy Proficiency testing (Genest, Sharon SktnHR)
8. Controls (Lorraine Cornett)
9. RE: Stability of Retic stain in Bone Marrow (Nacaela Johnson)
10. Histology Authors Needed! (Judi Bennett)
11. Re: Electron Microscopy Proficiency testing (Jan Shivers)
----------------------------------------------------------------------
Message: 1
Date: Sun, 8 Jan 2012 13:16:58 -0800 (PST)
From: naveeda arshad <naveedafahim <@t> yahoo.ca>
Subject: [Histonet] Re:ihc course
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1326057418.98464.YahooMailClassic <@t> web161706.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
does any one know about institute offering IHC coursethanks
--- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 98, Issue 10
To: histonet <@t> lists.utsouthwestern.edu
Received: Sunday, January 8, 2012, 4:16 PM
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: Quality assurance program for pathologists (Bob Richmond)
2. Re: finger nails (angela smith)
3. Re: finger nails (Michele Email)
4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey)
5. Advice needed the different types of cytomorphologic stains
(Gladys Lim)
----------------------------------------------------------------------
Message: 1
Date: Sat, 7 Jan 2012 13:40:26 -0500
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Quality assurance program for pathologists
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH57w10Dnku3UxFUbu_DNf7=5YrNYsjdCoxwO2eJbrTU=g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Diana McCaig (where?) asks: "Is anyone willing to share with me their
quality assurance/management
program for pathologists?"
Glad to know you're in charge of pathologists. (Also glad I'm nearly
73 years old, though not yet retired.)
I've worked in a few programs that did a 10% review of cases. If you
go this route, don't choose the cases at random, but ask the
pathologists to designate the cases as they do the day's work -
they'll catch a lot more problems that way. I've worked in a single
practice that did 100% second-pathologist review (before the case was
released), and I thought that was excessive.
Pathologists should be encouraged to document their internal
consultations - I mean when you pass a slide to the guy at the next
microscope and ask him "from the ear of a 70 year old man - do you
think there's enough here to call this a basal cell carcinoma?" Such
cases should be documented in a comment - I say "Dr. John Doe has seen
this material and concurs." Such cases are legitimately considered
part of a 10% review policy. I've worked in one large and highly
competent practice that documented internal consultation very
meticulously, and one of their QA guidelines was that 2.8% of their
cases document internal consultation.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 2
Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>, Michele Carr
<michelecarr10 <@t> yahoo.com>, Rene J Buesa <rjbuesa <@t> yahoo.com>
Message-ID:
<1325977229.85027.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.
--- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
Date: Friday, January 6, 2012, 3:45 PM
See attachment!
René J.
--- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
From: Michele Carr <michelecarr10 <@t> yahoo.com>
Subject: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Date: Friday, January 6, 2012, 1:06 PM
Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
Michele Carr
Medical Laboratory Services
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Sat, 7 Jan 2012 16:40:39 -0800
From: Michele Email <michelecarr10 <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: angela smith <we3smitty <@t> yahoo.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AB689FDC-FF01-4F9F-8BE7-FDABC2A051BD <@t> yahoo.com>
Content-Type: text/plain; charset=utf-8
Thanks everyone for the tips will try the Nair next time around.
Michele Carr
Sent from my iPad
On Jan 7, 2012, at 3:00 PM, angela smith <we3smitty <@t> yahoo.com> wrote:
> Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.
>
> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
> Date: Friday, January 6, 2012, 3:45 PM
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Sat, 7 Jan 2012 20:04:29 -0800
From: Madeleine Huey <madeleinehuey <@t> gmail.com>
Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAF2e4CJ2gYRkvpcEXq8DpOPA1D=ZWwqf-AvSeQYC4A1km5cW4Q <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Michele,
This is our procedure for toe & finger nails;
1) Pre-fix nails in 10% NFB as usual
2) Soak fixed nails in NAIR or any counter nail remover until soften
or bend easily (toe nail take longer; thickness dependent)
3) wash nail with water
4) Process in tissue processor
5) embed & cut
Note; we found soften with NAIR before processing work the best.
If NAIR is used after processing;
1) cut nail on charge slides (+)
2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly
3) bake jar in ~ 60c over for ~ 30 min
4) cool & open jar in fume hood ~ 5 min
5) stain as usual
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Sat, Jan 7, 2012 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. finger nails (Michele Carr)
> 2. Temporary & Direct hire PA(ASCP) for February/March (Refer a
> Friend) (Cheryl)
> 3. Saffron (Gagnon, Eric)
> 4. Re: finger nails (Rene J Buesa)
> 5. Quality assurance program for pathologists (Diana McCaig)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST)
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1325873200.90291.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
>
> ------------------------------
>
> Message: 2
> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST)
> From: Cheryl <tkngflght <@t> yahoo.com>
> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for
> February/March (Refer a Friend)
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1325875872.67239.YahooMailNeo <@t> web39404.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello 'Netters-
>
> Do you know any temp/travel PAs? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!! If you refer someone we help, you get a referral bonus (keep it or share it with your friend!)
>
> 1. Temp traveler for at least 4 weeks in mid-February.
> 2. Direct Hire in 6 different institutions around the US. Entry through Supervisory.
>
> As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide.
>
> Give me a call--make sure those you refer mention your name--I LIKE writing referral checks!
>
> Cheryl
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab by helping one GREAT Tech at a time.
> 281.852.9457 Office
> 800.756.3309 Phone & Fax
> admin <@t> fullstaff.org
>
> Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews <@t> fullstaff.org. Please include your name and specialty in the body of the email.
>
> ------------------------------
>
> Message: 3
> Date: Fri, 6 Jan 2012 15:19:18 -0500
> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
> Subject: [Histonet] Saffron
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F93BD6329FC3AE4C8DB116B985FBC3134D1D4FF7 <@t> KGHMAIL.KGH.ON.CA>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Beth, as Bob Richmond has noted regarding saffron,
>
> "The most common use is as the hematoxylin-phloxin-saffron (HPS)
> trichrome stain. It was in use as a general oversight stain in a few
> pathology services when I was a resident in the 1960's..."
>
> and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain.
>
> We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change.
>
> I still think the wafting of the boiling saffron is quite a pleasant aroma.
>
> Eric Gagnon MLT
> Histology Laboratory
> Kingston General Hospital
> Kingston, Ontario, Canada
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>, Michele Carr
> <michelecarr10 <@t> yahoo.com>
> Message-ID:
> <1325882708.34952.YahooMailClassic <@t> web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 6 Jan 2012 17:06:19 -0500
> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] Quality assurance program for pathologists
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DCFD9E6A390E294AAF3A2561CD32E5C417A90091 <@t> ckhamail1.ckha.on.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone willing to share with me their quality assurance/management
> program for pathologists.
>
>
>
> Sincere thanks
>
> Diana
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 98, Issue 9
> ***************************************
------------------------------
Message: 5
Date: Mon, 9 Jan 2012 00:18:08 +0800
From: Gladys Lim <gladys.science <@t> gmail.com>
Subject: [Histonet] Advice needed the different types of
cytomorphologic stains
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAJdQ0jWODg74_Vr5E9eSJ-JqQAs7ZZ3PVdedTBaKH3enh1DKkA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear all,
I am relatively new to this area of staining approach and therefore, I need
some advise on the different types of cytomorphologic stains that are
available.
(1) Is it necessary to air-dry your sample prior to staining with any
Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)?
(2) Has anyone tried using the Romanowski stains on sample that were not
air-dried? What was the outcome of the staining?
(3) Were there any distinct difference in terms of staining among the
different types of white blood cells vs. malignant cancer cells?
(4) Wouldn't air-drying of sample prior to Romanowski stain change the
morphology of cells?
(5) Any recommendation for other types of stains if I want to differentiate
white blood cells from cancer cells, without any air-drying steps involved?
Any advice is greatly appreciated.
Thanks.
Regards,
Gladys
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 98, Issue 10
****************************************
------------------------------
Message: 2
Date: Sun, 8 Jan 2012 14:27:29 -0800 (PST)
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] Re: Quality assurance program for pathologists
To: Bob Richmond <rsrichmond <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1326061649.24557.YahooMailNeo <@t> web112301.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dr Richmond,
You always please me with your replies. You are such a value to these forums. Let me, I'm sure for the thousant time tell you how much you are appreciated.
You clearly understand "Quality"
as most any Pathologist that Ive ever been subject to.
As you know 10% review is the minumum
100%........... yep!~ Ive seen that.. what a marvel of respect did that get from little ole me
Yes, excessive, we both agree
I have to say though to Diana, that usually the Pathologist have thier own QA program that monitors thier work. Ive never seen anything different. Even the lone Path.
Its always a good idea to communicate with them
They may already have the answer to your questions?
Kim D
________________________________
From: Bob Richmond <rsrichmond <@t> gmail.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Saturday, January 7, 2012 1:40 PM
Subject: [Histonet] Re: Quality assurance program for pathologists
Diana McCaig (where?) asks: "Is anyone willing to share with me their
quality assurance/management
program for pathologists?"
Glad to know you're in charge of pathologists. (Also glad I'm nearly
73 years old, though not yet retired.)
I've worked in a few programs that did a 10% review of cases. If you
go this route, don't choose the cases at random, but ask the
pathologists to designate the cases as they do the day's work -
they'll catch a lot more problems that way. I've worked in a single
practice that did 100% second-pathologist review (before the case was
released), and I thought that was excessive.
Pathologists should be encouraged to document their internal
consultations - I mean when you pass a slide to the guy at the next
microscope and ask him "from the ear of a 70 year old man - do you
think there's enough here to call this a basal cell carcinoma?" Such
cases should be documented in a comment - I say "Dr. John Doe has seen
this material and concurs." Such cases are legitimately considered
part of a 10% review policy. I've worked in one large and highly
competent practice that documented internal consultation very
meticulously, and one of their QA guidelines was that 2.8% of their
cases document internal consultation.
Bob Richmond
Samurai Pathologist
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Sun, 8 Jan 2012 14:40:46 -0800 (PST)
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] Re:ihc course
To: naveeda arshad <naveedafahim <@t> yahoo.ca>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1326062446.12201.YahooMailNeo <@t> web112306.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
You can get IHC education from most of the vendors. If you cant go that route, then there are many good books and subscriber publications out.
If you really want to be on top of the subject, do what I did many many years ago.
It's called do it yourself education
Get one of the books they give you to order from and learn from it. It always tells you what each antibody stains for, whats the control etc
You actually can go online to any of the major sites such as DAKO, LEICA or VENTANA and see thier antibody list with all the applied info for them.
In America you can also obtain knowledge from the NSH( http://www.nsh.org/ ) or as in Florida we have FSH (http://www.fshgroup.org/ )
Much Luck
Kim D
________________________________
From: naveeda arshad <naveedafahim <@t> yahoo.ca>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Sunday, January 8, 2012 4:16 PM
Subject: [Histonet] Re:ihc course
does any one know about institute offering IHC coursethanks
--- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 98, Issue 10
To: histonet <@t> lists.utsouthwestern.edu
Received: Sunday, January 8, 2012, 4:16 PM
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: Quality assurance program for pathologists (Bob Richmond)
2. Re: finger nails (angela smith)
3. Re: finger nails (Michele Email)
4. Re: Histonet Digest, Vol 98, Issue 9 (Madeleine Huey)
5. Advice needed the different types of cytomorphologic stains
(Gladys Lim)
----------------------------------------------------------------------
Message: 1
Date: Sat, 7 Jan 2012 13:40:26 -0500
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Quality assurance program for pathologists
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH57w10Dnku3UxFUbu_DNf7=5YrNYsjdCoxwO2eJbrTU=g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Diana McCaig (where?) asks: "Is anyone willing to share with me their
quality assurance/management
program for pathologists?"
Glad to know you're in charge of pathologists. (Also glad I'm nearly
73 years old, though not yet retired.)
I've worked in a few programs that did a 10% review of cases. If you
go this route, don't choose the cases at random, but ask the
pathologists to designate the cases as they do the day's work -
they'll catch a lot more problems that way. I've worked in a single
practice that did 100% second-pathologist review (before the case was
released), and I thought that was excessive.
Pathologists should be encouraged to document their internal
consultations - I mean when you pass a slide to the guy at the next
microscope and ask him "from the ear of a 70 year old man - do you
think there's enough here to call this a basal cell carcinoma?" Such
cases should be documented in a comment - I say "Dr. John Doe has seen
this material and concurs." Such cases are legitimately considered
part of a 10% review policy. I've worked in one large and highly
competent practice that documented internal consultation very
meticulously, and one of their QA guidelines was that 2.8% of their
cases document internal consultation.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 2
Date: Sat, 7 Jan 2012 15:00:29 -0800 (PST)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>, Michele Carr
<michelecarr10 <@t> yahoo.com>, Rene J Buesa <rjbuesa <@t> yahoo.com>
Message-ID:
<1325977229.85027.YahooMailClassic <@t> web125401.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.
--- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
Date: Friday, January 6, 2012, 3:45 PM
See attachment!
René J.
--- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
From: Michele Carr <michelecarr10 <@t> yahoo.com>
Subject: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Date: Friday, January 6, 2012, 1:06 PM
Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
Michele Carr
Medical Laboratory Services
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Sat, 7 Jan 2012 16:40:39 -0800
From: Michele Email <michelecarr10 <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: angela smith <we3smitty <@t> yahoo.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AB689FDC-FF01-4F9F-8BE7-FDABC2A051BD <@t> yahoo.com>
Content-Type: text/plain; charset=utf-8
Thanks everyone for the tips will try the Nair next time around.
Michele Carr
Sent from my iPad
On Jan 7, 2012, at 3:00 PM, angela smith <we3smitty <@t> yahoo.com> wrote:
> Have you tried nairing it prior to fixation and processing? We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness) then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.
>
> --- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
> Date: Friday, January 6, 2012, 3:45 PM
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Sat, 7 Jan 2012 20:04:29 -0800
From: Madeleine Huey <madeleinehuey <@t> gmail.com>
Subject: [Histonet] Re: Histonet Digest, Vol 98, Issue 9
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAF2e4CJ2gYRkvpcEXq8DpOPA1D=ZWwqf-AvSeQYC4A1km5cW4Q <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Michele,
This is our procedure for toe & finger nails;
1) Pre-fix nails in 10% NFB as usual
2) Soak fixed nails in NAIR or any counter nail remover until soften
or bend easily (toe nail take longer; thickness dependent)
3) wash nail with water
4) Process in tissue processor
5) embed & cut
Note; we found soften with NAIR before processing work the best.
If NAIR is used after processing;
1) cut nail on charge slides (+)
2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly
3) bake jar in ~ 60c over for ~ 30 min
4) cool & open jar in fume hood ~ 5 min
5) stain as usual
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology)
madeleine_h <@t> elcaminohospital.org
On Sat, Jan 7, 2012 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. finger nails (Michele Carr)
> 2. Temporary & Direct hire PA(ASCP) for February/March (Refer a
> Friend) (Cheryl)
> 3. Saffron (Gagnon, Eric)
> 4. Re: finger nails (Rene J Buesa)
> 5. Quality assurance program for pathologists (Diana McCaig)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST)
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1325873200.90291.YahooMailNeo <@t> web120704.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
>
> ------------------------------
>
> Message: 2
> Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST)
> From: Cheryl <tkngflght <@t> yahoo.com>
> Subject: [Histonet] Temporary & Direct hire PA(ASCP) for
> February/March (Refer a Friend)
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1325875872.67239.YahooMailNeo <@t> web39404.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello 'Netters-
>
> Do you know any temp/travel PAs? We've got a couple of different openings for the right people. In this economy who doesn't want a few more options??!! If you refer someone we help, you get a referral bonus (keep it or share it with your friend!)
>
> 1. Temp traveler for at least 4 weeks in mid-February.
> 2. Direct Hire in 6 different institutions around the US. Entry through Supervisory.
>
> As a working tech I know a lot about the jobs before we submit you--and we respect that it's your life we're talking about--we help make sure you have lots of choices: you decide.
>
> Give me a call--make sure those you refer mention your name--I LIKE writing referral checks!
>
> Cheryl
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab by helping one GREAT Tech at a time.
> 281.852.9457 Office
> 800.756.3309 Phone & Fax
> admin <@t> fullstaff.org
>
> Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews <@t> fullstaff.org. Please include your name and specialty in the body of the email.
>
> ------------------------------
>
> Message: 3
> Date: Fri, 6 Jan 2012 15:19:18 -0500
> From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
> Subject: [Histonet] Saffron
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F93BD6329FC3AE4C8DB116B985FBC3134D1D4FF7 <@t> KGHMAIL.KGH.ON.CA>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Beth, as Bob Richmond has noted regarding saffron,
>
> "The most common use is as the hematoxylin-phloxin-saffron (HPS)
> trichrome stain. It was in use as a general oversight stain in a few
> pathology services when I was a resident in the 1960's..."
>
> and is still in use here in Ontario by about 10% of the province's histology laboratories as a routine oversight stain.
>
> We have gone the same route as other respondents have noted over the years, utilizing a variety of suppliers, including a Mediterranean health food store in Ottawa for saffron. Now we are using the Sun Brand saffron produced in Spain, that is available at the check-out counter at our local bulk foods store. One might think that there would be a total shift to H&E as a routine stain, especially with automated stainers becoming prevalent, but we have successfully automated the stain on successive automated stainers. Since our newest pathologists were trained as residents here, they are quite used to HPS, and there appears to be little impetus to change.
>
> I still think the wafting of the boiling saffron is quite a pleasant aroma.
>
> Eric Gagnon MLT
> Histology Laboratory
> Kingston General Hospital
> Kingston, Ontario, Canada
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>, Michele Carr
> <michelecarr10 <@t> yahoo.com>
> Message-ID:
> <1325882708.34952.YahooMailClassic <@t> web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> See attachment!
> René J.
>
> --- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:
>
>
> From: Michele Carr <michelecarr10 <@t> yahoo.com>
> Subject: [Histonet] finger nails
> To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Date: Friday, January 6, 2012, 1:06 PM
>
>
> Hi everyone was wondering what you do to get the nail from washing off the slide during staining. The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it? Thanks in advance for all your responses.
> Michele Carr
> Medical Laboratory Services
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 6 Jan 2012 17:06:19 -0500
> From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
> Subject: [Histonet] Quality assurance program for pathologists
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DCFD9E6A390E294AAF3A2561CD32E5C417A90091 <@t> ckhamail1.ckha.on.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone willing to share with me their quality assurance/management
> program for pathologists.
>
>
>
> Sincere thanks
>
> Diana
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 98, Issue 9
> ***************************************
------------------------------
Message: 5
Date: Mon, 9 Jan 2012 00:18:08 +0800
From: Gladys Lim <gladys.science <@t> gmail.com>
Subject: [Histonet] Advice needed the different types of
cytomorphologic stains
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAJdQ0jWODg74_Vr5E9eSJ-JqQAs7ZZ3PVdedTBaKH3enh1DKkA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear all,
I am relatively new to this area of staining approach and therefore, I need
some advise on the different types of cytomorphologic stains that are
available.
(1) Is it necessary to air-dry your sample prior to staining with any
Romanowski stains (eg. Giemsa, Wright-Giemsa etc.)?
(2) Has anyone tried using the Romanowski stains on sample that were not
air-dried? What was the outcome of the staining?
(3) Were there any distinct difference in terms of staining among the
different types of white blood cells vs. malignant cancer cells?
(4) Wouldn't air-drying of sample prior to Romanowski stain change the
morphology of cells?
(5) Any recommendation for other types of stains if I want to differentiate
white blood cells from cancer cells, without any air-drying steps involved?
Any advice is greatly appreciated.
Thanks.
Regards,
Gladys
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 98, Issue 10
****************************************
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Sun, 8 Jan 2012 23:32:05 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Re:ihc course
To: "Kim Donadio" <one_angel_secret <@t> yahoo.com>, "naveeda arshad"
<naveedafahim <@t> yahoo.ca>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C1814EF6B2EF45A2B034C426204CB100 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
NSH has a lot of IHC teleconferences:
http://s3.goeshow.com/nsh/NSHTC2012/ereg949997.cfm?pg=home
Under Register, you can download the brochure.
NSH also has some IHC forums: July 13-14, 2012 in Hartford, CT. Not much
information available yet, but keep checking.
http://www.nsh.org/content/immunohistochemistry-forum
Dako has a lot of downloadable booklets
http://www.dako.com/us/index/knowledgecenter.htm
But as for a college offering courses/degree in IHC - I don't know of any.
There are courses on Immunology in general, and there are courses for the
med tech training programs, specific to their immunology. For the histotech
programs that are college/university based, the IHC is built into the
curriculum, usually part of the special stains class, and you have to be
accepted into the histotech program. So most histotechs learn it on-the-job,
and attending their state and national symposiums.
Peggy Wenk, HTL(ASCP)SLS
-----Original Message-----
From: Kim Donadio
Sent: Sunday, January 08, 2012 5:40 PM
To: naveeda arshad ; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re:ihc course
You can get IHC education from most of the vendors. If you cant go that
route, then there are many good books and subscriber publications out.
If you really want to be on top of the subject, do what I did many many
years ago.
It's called do it yourself education
Get one of the books they give you to order from and learn from it. It
always tells you what each antibody stains for, whats the control etc
You actually can go online to any of the major sites such as DAKO, LEICA or
VENTANA and see thier antibody list with all the applied info for them.
In America you can also obtain knowledge from the NSH( http://www.nsh.org/ )
or as in Florida we have FSH (http://www.fshgroup.org/ )
Much Luck
Kim D
________________________________
From: naveeda arshad <naveedafahim <@t> yahoo.ca>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Sunday, January 8, 2012 4:16 PM
Subject: [Histonet] Re:ihc course
does any one know about institute offering IHC coursethanks
--- On Sun, 1/8/12, histonet-request <@t> lists.utsouthwestern.edu
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu
<histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 98, Issue 10
To: histonet <@t> lists.utsouthwestern.edu
Received: Sunday, January 8, 2012, 4:16 PM
------------------------------
Message: 5
Date: Mon, 09 Jan 2012 09:25:01 -0400
From: "Elizabeth Chatfield" <epchatfield <@t> ihis.org>
Subject: [Histonet] Stability of Retic stain in Bone Marrow
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4F0AB26D020000BC00014C4C <@t> coregwia.peigov>
Content-Type: text/plain; charset=US-ASCII
Hi Everyone,
I'm wondering if anyone has experienced issues with the stability of reticulin (Gordon and Sweets method) in bone marrow. We are seeing silver precipitant after ~1 week on our patient sections - our control is fine.
Cheers,
Elizabeth Peyton-Chatfield
Histology Supervisor
Queen Elizabeth Hospital
Charlottetown, PE
-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.
------------------------------
Message: 6
Date: Mon, 9 Jan 2012 09:32:12 -0600
From: "Genest, Sharon SktnHR"
<Sharon.Genest <@t> saskatoonhealthregion.ca>
Subject: [Histonet] Respirators and Routine Histology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B7F15445A710BA4FA7B48F2C55134AD40655D182 <@t> lou.sktnhr.ca>
Content-Type: text/plain; charset="us-ascii"
Amy
We use respirators when changing stainers and processors. Our Gross
Technologists work in chemical hoods, our special stains and
coverslipping are performed in chemical hoods. We anually measure air
quality and our hood air flow.
Sharon Genest
Anatomic Pathology
Process Improvement
Saskatoon Health Region
306-655-8242
sharon.genest <@t> saskatoonhealthregion.ca
------------------------------
Message: 7
Date: Mon, 9 Jan 2012 09:36:10 -0600
From: "Genest, Sharon SktnHR"
<Sharon.Genest <@t> saskatoonhealthregion.ca>
Subject: [Histonet] Electron Microscopy Proficiency testing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B7F15445A710BA4FA7B48F2C55134AD40655D183 <@t> lou.sktnhr.ca>
Content-Type: text/plain; charset="us-ascii"
Does anyone subscribe to a program for proficiency testing for Electron
Microscopy?
Sharon Genest
Anatomic Pathology
Process Improvement
Saskatoon Health Region
306-655-8242
sharon.genest <@t> saskatoonhealthregion.ca
------------------------------
Message: 8
Date: Mon, 9 Jan 2012 10:44:14 -0500
From: Lorraine Cornett <cornettl <@t> hotmail.com>
Subject: [Histonet] Controls
To: Histonet Listserve <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY169-W27FC15BA05C1A5FC2A7B82D6980 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
We are in need of Aspergillus and Amyloid controls. Does anyone on the histonet have any suggestions, or overabundance that they could share with us?
Thanks,
Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349
------------------------------
Message: 9
Date: Mon, 9 Jan 2012 10:00:48 -0600
From: "Nacaela Johnson" <njohnson <@t> kcskincenter.com>
Subject: RE: [Histonet] Stability of Retic stain in Bone Marrow
To: "'Elizabeth Chatfield'" <epchatfield <@t> ihis.org>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000101cccee7$db9e1780$92da4680$@com>
Content-Type: text/plain; charset="US-ASCII"
My experience with the Gordon and Sweets Reticulin is 1 to 2 days max. I
found better results and stability with the Gomoris Retic from Poly
Scientific.
Nacaela Johnson, B.S. HTL (ASCP)
Histology Supervisor
Kansas City Skin & Cancer Center, LLC
5810 NW Barry Rd, Ste 100
Kansas City, MO 64154
ph: 816 584 8100
fx: 816 584 8106
em: njohnson <@t> kcskincenter.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chatfield
Sent: Monday, January 09, 2012 7:25 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stability of Retic stain in Bone Marrow
Hi Everyone,
I'm wondering if anyone has experienced issues with the stability of
reticulin (Gordon and Sweets method) in bone marrow. We are seeing silver
precipitant after ~1 week on our patient sections - our control is fine.
Cheers,
Elizabeth Peyton-Chatfield
Histology Supervisor
Queen Elizabeth Hospital
Charlottetown, PE
-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged
information intended for a specific individual or organization. If you have
received this communication in error, please notify the sender immediately.
If you are not the intended recipient, you are not authorized to use,
disclose, distribute, copy, print or rely on this email, and should promptly
delete this email from your entire computer system.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mon, 9 Jan 2012 11:55:34 -0500
From: Judi Bennett <judi <@t> medialabinc.net>
Subject: [Histonet] Histology Authors Needed!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC2QUZ8o1==F5Mvs7kX6BEfVMPjcus8RZ85ZZ+=D_jh7Y6FY7w <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252
*HISTOLOGY* authors needed ... especially for *MOLECULAR* courses!
Actively seeking authors to write online *histology courses* for *MediaLab*!
MediaLab is a leading publisher of online continuing education (CE) courses
and competency assessments. Our online products are used at more than 2,000
laboratories and university CLS programs worldwide.
This is a great opportunity to:
- Gain* resume-boosting publishing experience*
- *Earn honorariums* for your participation
- Fill the *critical need for quality histology CE credits*
Authors can take advantage of MediaLab's online CourseBuilder to *write
courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive
interface similar to Microsoft Word or PowerPoint. Authors can quickly
create content pages, practice questions, and exam questions, and upload
relevant images.
Courses developed by MediaLab are *featured on our websites MediaLabInc.net
and LabCE.com*. Questions from these courses also become part of the
LabCE.com Quiz Game, with over 1,000 daily players. Authors and reviewers
may also *contribute to other online programs that we develop on behalf of
major laboratory partners*.
To learn more about becoming a MediaLab author for histology courses, visit
our online information page at www.medialbinc.net/authors.aspx . Please
contact Judi Bennett at judi <@t> medialabinc.net or call 877-776-8460, ext.
721.
Judi Bennett
Program Director, MediaLab, Inc.
--
Judi Bennett, BSM, MT
MediaLab, Inc.
e-mail judi <@t> medialabinc.net
Phone (877) 776-8460 ext. 721
cell phone 404-915-2999
fax (678) 401-0284
------------------------------
Message: 11
Date: Mon, 9 Jan 2012 11:25:52 -0600
From: Jan Shivers <shive003 <@t> umn.edu>
Subject: Re: [Histonet] Electron Microscopy Proficiency testing
To: "Genest, Sharon SktnHR" <Sharon.Genest <@t> saskatoonhealthregion.ca>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAEoC1q1QsJVkgN8iE+XYOdHEEfm4G9nmPtUXtN2DziaBbhH0VQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We participate in the External Quality Assessment Scheme in EM Virus
Diagnostics (EQA-EMV) administered through the Robert Koch Institut of
Berlin, Germany. It tests proficiency only on infectious disease
identification.
Jan Shivers
Section Head - EM
University of Minnesota
Veterinary Diagnostic Laboratory
St. Paul, MN, USA
On Mon, Jan 9, 2012 at 9:36 AM, Genest, Sharon SktnHR <
Sharon.Genest <@t> saskatoonhealthregion.ca> wrote:
> Does anyone subscribe to a program for proficiency testing for Electron
> Microscopy?
>
> Sharon Genest
> Anatomic Pathology
> Process Improvement
> Saskatoon Health Region
> 306-655-8242
> sharon.genest <@t> saskatoonhealthregion.ca
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 98, Issue 11
****************************************
More information about the Histonet
mailing list