[Histonet] finger nails

[angela smith]

Have you tried nairing it prior to fixation and processing?  We have validated that applying a very thick coat of nair on the nail prior to fixation for 15 min to 1 hour (depending on nail size and thickness)  then rinse with tap, then place in formalin and process. We do not have any issues with nails falling off. Also make sure you use charged slides.

--- On Fri, 1/6/12, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>, "Michele Carr" <michelecarr10 <@t> yahoo.com>
Date: Friday, January 6, 2012, 3:45 PM

See attachment!
René J.

--- On Fri, 1/6/12, Michele Carr <michelecarr10 <@t> yahoo.com> wrote:


From: Michele Carr <michelecarr10 <@t> yahoo.com>
Subject: [Histonet] finger nails
To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Date: Friday, January 6, 2012, 1:06 PM


Hi everyone was wondering what you do to get the nail from washing off the slide during staining.  The nail is extremely hard and seems to be washing each time. Could I soften it prior to staining and what do you use to soften it?  Thanks in advance for all your responses.  
Michele Carr
Medical Laboratory Services
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