[Histonet] Clearing NBT/BCIP precipitate from wax sections

Andrew Gillis jag93 <@t> cam.ac.uk
Thu Jan 5 14:18:06 CST 2012


I have some paraffin sections that I've used for immunohistochemistry 
(using an alkaline phosphatase-conjugated secondary antibody and 
NBT/BCIP as a substrate), and coverslipped with an aqueous mounting 
medium (Fluoromount G). I would now like to de-coverslip and re-stain 
these sections with a different histochemical protocol (i.e. Masson's 
trichrome or H&E). Does anybody know if there is a way to "clear away" 
the colour reaction precipitate from my previous IHC? If, after 
decoverslipping, I dehydrate and then rehydrate the sections, will this 
do the job?

Any advice would be greatly appreciated.

Thanks very much,

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