[Histonet] Amazing

[Bernice Frederick]

We use .5%  acid alcohol ( hydrochloric) with Harris hemo. No problems.
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, January 05, 2012 10:17 AM
To: histonet <@t> lists.utsouthwestern.edu; Sarah Dysart
Subject: Re: [Histonet] Amazing

You can use either acetic or hydrochloric, both will do. The strength is 1% BUT it does not matter. As a matter of fact the weaker the solution the better. Remember that the acid solution (that can be made with 7ethanolanol even better) is used to differentiate progressivesive hematoxylin, like Harris, and the weaker it is the more control you have with the differentiation.
A weak solution allows you to dip the slides several times until you obtain the desired reddish hue on the sections that signals when the differentiation is completed.
If the solution is too strong you will have to take the slides often precipitouslyusly and some sections will be have a weak nuclear staining.
So, use acetic at 1% in 70% ethanol  That is what I used to prepare.
René J.

--- On Thu, 1/5/12, Sarah Dysart <sdysart <@t> mirnarx.com> wrote:


From: Sarah Dysart <sdysart <@t> mirnarx.com>
Subject: [Histonet] Amazing
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, January 5, 2012, 10:59 AM


I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away.  That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up).  I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well.  I want to say it was like a 1% acid solution in alcohol??  What was the acid?  For some reason my brain says glacial acetic...but time has made me forget.  Is the alcohol you mix it in 100% or something lower with a water content to it?  Please help my alzheimers =) Happy Thursday!!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

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