[Histonet] Nerve Fiber Density Testing

[Adrienne Kavanagh]

Hi All,

I am setting up for a nerve fiber density test.  The specifics of the procedure I have been provided with are vague.
 
It basically involves taking a punch biopsy of skin, fixing in PLP, cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with dry ice, and section using a sliding microtome at 50 microns.  Then staining the free-floating sections with a PGP 9.5 antibody.  
 
 
If anyone is performing this testing, I would appreciate your response (you may contact me privately if you wish).
 
Thanks!
 
Adrienne