[Histonet] Parameters for alcohol on Processor

Cruise, Karen kcruise <@t> path.wustl.edu
Mon Feb 27 15:41:33 CST 2012

Hello Histonetters,

Could someone please advise me on established guidelines for changing the alcohol on the processor. We process a very small amount of blocks weekly on our ASP300S. Approximately 20 blocks per week. We have decided to use a hydrometer to test our 70% thru 100% alcohols, rather than base our changing reagents on number of blocks processed. My questions are, does a (1) % point decrease necessitate a change ? What are the parameters for changing each alcohol ?

Thanking you in advance,


Karen E. Cruise

Histologist (Research Technician II)

Washington University School of Medicine

Laboratory for Translational Pathology

216 S. Kingshighway Room 2332

St. Louis, MO 63110

(314) 454-8636 Phone

(314) 454-5525 Fax

kcruise <@t> path.wustl.edu<mailto:kcruise <@t> path.wustl.edu>

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