[Histonet] Re: Histonet Digest, Vol 99, Issue 30

Thu Feb 23 12:36:02 CST 2012

Message 5: xylene

Xylene is toxic and you should always wear gloves and a face mask when dealing with it. Dermal absorption is a lot slower than inhalation, but contact with organic solvents such as xylene is bad in general. With the issue of latex residue left on the slide, try using nitrile gloves. We use nitrile in our lab and we never have an issue with residue left on the slides. You may also want to wear two gloves on the hand that might come in contact with xylene because it can eat through gloves. I haven't found anything linking xylene as a human carcinogen, but it's toxic to the central nervous system as well as a known teratogen, so limiting any contact is advised. You can also use histoclear, another organic solvent that works really well that is far less toxic than xylene.

On Feb 23, 2012, at 1:00 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:

> Send Histonet mailing list submissions to
>    histonet <@t> lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>    histonet-request <@t> lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>    histonet-owner <@t> lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. RE: Re: Histonet Digest, Vol 99, Issue 28 (Sheila Fonner)
>   2. ADIPOPHILIN (Walls, Rachel)
>   3. out of office (Marilyn.A.Weiss <@t> kp.org)
>   4. RE: Does xylene cause skin cancer? (Mayer,Toysha N)
>   5. Histology Lab Donations (Salim Yalcin Inan)
>   6. Cleaning Bond Bulk Reagent Containers (Konop, Nicole)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Thu, 23 Feb 2012 06:44:25 -0500
> From: "Sheila Fonner" <sfonner <@t> labpath.com>
> Subject: RE: [Histonet] Re: Histonet Digest, Vol 99, Issue 28
> To: "'Madeleine Huey'" <madeleinehuey <@t> gmail.com>,
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000001ccf220$7e8ff2a0$7bafd7e0$@com>
> Content-Type: text/plain;    charset="iso-8859-1"
> 
> ? You use regular superfrost plus slides for temperature verification of the
> thermal pads?  The slides to use are the superfrost plus slides from Ventana
> that have a qc'd strip on them that will turn black if the temperatures of
> pads are at acceptable levels.  How do you prove that with a plain
> superfrost plus slide?
> 
> We use the suggested qc slides, but only do the test twice/yr instead of
> quarterly.  We re-wrote our procedure to indicate that this is what we do.
> We then copy the slides onto paper and file for documentation purposes. You
> could also keep the slides, if you have the room to file and store them.
> CAP requires the written procedure as well as documentation that it has been
> done.
> 
> Sheila, HT (ASCP)
> KDL Pathology
> Knoxville, TN
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madeleine
> Huey
> Sent: Wednesday, February 22, 2012 9:22 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet Digest, Vol 99, Issue 28
> 
> Message: 15
> Date: Wed, 22 Feb 2012 15:26:43 +0000
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] Temp verifier slides - Ventana equipment
> To: "histonet <@t> lists.utsouthwestern.edu"
>       <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <5A33C952BB67F4468AF1F36D739212BC115ECA7E <@t> JERRY.Gia.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> Do y'all run the temp verifier slides for the quarterly maintenance for the
> Ventana XT and Ultra?  Those slides are mighty expensive to buy every 3
> months, esp if you have multiple pieces of equipment.
> 
> Amber,
> 
> We used regular Superfrost Plus slide (ie. vWR & Fisher) & they work just
> fine.
> 
> Madeleine Huey BS, HTL (ASCP) QIHC
> Supervisor - Pathology (IPOX & Histology) madeleine_h <@t> elcaminohospital.org
> 
> On Wed, Feb 22, 2012 at 10:01 AM,
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>> Send Histonet mailing list submissions to
>> � � � �histonet <@t> lists.utsouthwestern.edu
>> 
>> To subscribe or unsubscribe via the World Wide Web, visit
>> � � � �http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>> � � � �histonet-request <@t> lists.utsouthwestern.edu
>> 
>> You can reach the person managing the list at
>> � � � �histonet-owner <@t> lists.utsouthwestern.edu
>> 
>> When replying, please edit your Subject line so it is more specific 
>> than "Re: Contents of Histonet digest..."
>> 
>> 
>> Today's Topics:
>> 
>> � 1. Seeking a Histology Supervisor in Connecticut (Darcy Bloch)
>> � 2. RE: Processor Question (Davide Costanzo)
>> � 3. Job Opening in Orange county California (Paula Lucas)
>> � 4. Cytology CSF Cell Pellets made from Histogel (Amos Brooks)
>> � 5. Does xylene cause skin cancer? (Jenny Vega)
>> � 6. RE: Does xylene cause skin cancer? (Settembre, Dana)
>> � 7. Storage of Frozen Tissues (Dennis Hahn)
>> � 8. Re: Does xylene cause skin cancer? (Rene J Buesa)
>> � 9. RE: Does xylene cause skin cancer? (Debbie Faichney)
>> �10. Re: Storage of Frozen Tissues (Rene J Buesa)
>> �11. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam)
>> �12. Re: Cytology CSF Cell Pellets made from Histogel (Kim Merriam)
>> �13. RE: Does xylene cause skin cancer? (Hilary Smith)
>> �14. Histotech & Histology Supervisor Job in Naples, FL
>> � � �(Melissa Phelan)
>> �15. Temp verifier slides - Ventana equipment (Amber McKenzie)
>> �16. RE: Does xylene cause skin cancer? (Goins, Tresa)
>> 
>> 
>> ----------------------------------------------------------------------
>> 
>> Message: 1
>> Date: 21 Feb 2012 13:08:28 -0500
>> From: "Darcy Bloch" <darcyb <@t> slonepartners.com>
>> Subject: [Histonet] Seeking a Histology Supervisor in Connecticut
>> To: histonet <@t> lists.utsouthwestern.edu
>> Message-ID:
>> � � � �<mailman.0.1329933601.18415.histonet <@t> lists.utsouthwestern.edu>
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> 
>> � Slone �Partners �seeks �a �Histology Supervisor for our hospital 
>> based
>> � laboratory
>> 
>> 
>> The �successful �candidate � redesign �and �be �great �at �managing
> change. � people in the department, including 2 supervisors. � experience is
> a plus.
>> 
>> 
>> 
>> Qualified � certification, �with � high-volume laboratory.
>> 
>> 
>> 
>> Special �features �of this position: � have the opportunity to help 
>> redesign this
>> 
>> 
>> If �you �meet these qualifications � for �this �position, �please �submit
> �your �resume � darcyb <@t> slonepartners.com.
>> 
>> 
>> 
>> If �you � wish �to be considered � Tara Kochis at tara <@t> slonepartners.com.
>> 
>> 
>> 
>> All inquiries are kept confidential.
>> 
>> 
>> ------------------------------
>> 
>> Message: 2
>> Date: Tue, 21 Feb 2012 10:33:27 -0800
>> From: Davide Costanzo <pathlocums <@t> gmail.com>
>> Subject: RE: [Histonet] Processor Question
>> To: "Gauch, Vicki" <GauchV <@t> mail.amc.edu>,
>> � � � �"histonet <@t> lists.utsouthwestern.edu"
>> � � � �<histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <-2296715788321707800 <@t> unknownmsgid>
>> Content-Type: text/plain; charset=ISO-8859-1
>> 
>> Design flaw in the screen display. It is in the way of the chamber 
>> when opening chamber. If your not careful you will break the screen. 
>> Happens fairly often.
>> 
>> Sent from my Windows Phone
>> From: Gauch, Vicki
>> Sent: 2/21/2012 9:18 AM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Processor Question Hi everyone, We are in the 
>> market for new processors...and I was wondering if anyone could give 
>> me some pros and cons for the Tissue Tek VIP 6 tissue processor - how 
>> reliable are they? Ease of use ? Any known problems? �Tissues process 
>> well? � You know....all the usual questions we all ask for new 
>> equipment.....
>> 
>> Thanks in advance for your help,
>> 
>> Vicki Gauch
>> AMCH
>> Albany, NY
>> 
>> 
>> 
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>> 
>> ------------------------------
>> 
>> Message: 3
>> Date: Tue, 21 Feb 2012 12:23:07 -0800
>> From: "Paula Lucas" <plucas <@t> biopath.org>
>> Subject: [Histonet] Job Opening in Orange county California
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <B4B63414CF0349E7A008F0FD4B17E64C <@t> biopath.local>
>> Content-Type: text/plain; � � � charset="US-ASCII"
>> 
>> A part-time histotech position is open in our histology laboratory.
>> 
>> Tuesday through Saturday starting at 5 am.
>> 
>> If anyone is interested, please either fax or email me your resume.
>> 
>> Thank you,
>> 
>> Paula Lucas
>> 
>> Bio-Path Medical Group
>> 
>> Fountain Valley, CA 92708
>> 
>> Fax: 714-755-2984
>> 
>> 
>> 
>> ------------------------------
>> 
>> Message: 4
>> Date: Tue, 21 Feb 2012 17:22:57 -0500
>> From: Amos Brooks <amosbrooks <@t> gmail.com>
>> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
>> To: histonet <@t> lists.utsouthwestern.edu
>> Message-ID:
>> � � � �
>> <CAC95ki_YVmP7TXrDFnRbsbtDh5-hfZ2Wc_m+LzimtFq_71NOcg <@t> mail.gmail.com>
>> Content-Type: text/plain; charset=ISO-8859-1
>> 
>> Hi,
>> � � I haven't done a cellblock on CSF with Histogel, but I have has 
>> success with some fairly scanty cell culture specimens. A short 
>> processing cycle would be best. Try to make sure you have removed as 
>> much supernatant as possible to keep the gel from shriveling during 
>> processing. The IHC will be fine as long as the cells are suitably fixed
> ahead of time.
>> 
>> Here is a link to the packaging insert for Histogel which describes 
>> preparation of cell blocks.
>> https://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf
>> 
>> And here's a video of people using it for cytology specimens.
>> http://tinyurl.com/7jb522e
>> 
>> Amos
>> 
>> 
>> On Tue, Feb 21, 2012 at 1:01 PM,
>> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>> 
>>> Message: 15
>>> Date: Tue, 21 Feb 2012 11:26:10 -0600
>>> From: "Turner, Leandra" <LTurner1 <@t> seton.org>
>>> Subject: [Histonet] Cytology CSF Cell Pellets made from Histogel
>>> To: <histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID:
>>> � � � �<3D79F47DC92B204F9E5D35C885DFC5CB010AB869 <@t> AUSEX2VS1.seton.org>
>>> Content-Type: text/plain; � � � charset="us-ascii"
>>> 
>>> 
>>> 
>>> 
>>> 
>>> Hello Everyone,
>>> 
>>> � �I am trying to find out a few of things about making cell pellets 
>>> on cerebrospinal fluids. �I would like to know:
>>> 
>>> 
>>> 
>>> � � 1. If anyone has ever made cell pellets from CSF's using Histogel 
>>> and has any tips or procedures they could share?
>>> 
>>> 
>>> 
>>> � � 2. How to process the CSF pellets made with Histogel, do we need 
>>> a routine or stat process? �(we use a Sakura Tissue Tek)
>>> 
>>> 
>>> 
>>> � � 3. Can you do IHC staining on the pellets?
>>> 
>>> 
>>> 
>>> 
>>> 
>>> Thank you for any and all help in advance.
>>> 
>>> 
>>> 
>>> Leandra Turner, HT (ASPC)CM
>>> 
>> 
>> 
>> ------------------------------
>> 
>> Message: 5
>> Date: Tue, 21 Feb 2012 23:17:58 -0400
>> From: Jenny Vega <histotech411 <@t> gmail.com>
>> Subject: [Histonet] Does xylene cause skin cancer?
>> To: histonet <@t> lists.utsouthwestern.edu
>> Message-ID:
>> � � � �
>> <CADyQr2Cv-VdN=mPfebQbQJGNZQ=XQeAbfa7tqUbtNhK45u2byA <@t> mail.gmail.com>
>> Content-Type: text/plain; charset=ISO-8859-1
>> 
>> I am asking this because in my job we mount slides by hand, and my 
>> coworkers don't like to use gloves because it leaves a residue of 
>> latex in the back of the slides. I really don't feel comfortable 
>> mounting without gloves because I heard that xyelene can cause cancer. 
>> Some people I know personally has told me that this is not possible, 
>> but I read in some places that xylene could a possible carcinogen.
>> 
>> I have already gotten contact with xylene in my hands a couple of 
>> times and I am worried.
>> 
>> 
>> 
>> Thanks.
>> 
>> 
>> ------------------------------
>> 
>> Message: 6
>> Date: Wed, 22 Feb 2012 06:47:20 -0500
>> From: "Settembre, Dana" <settembr <@t> umdnj.edu>
>> Subject: RE: [Histonet] Does xylene cause skin cancer?
>> To: 'Jenny Vega' <histotech411 <@t> gmail.com>,
>> � � � �"histonet <@t> lists.utsouthwestern.edu"
>> � � � �<histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> � � � �
>> <B64B947688FB794A8C191D19F22927C80318E3F156 <@t> UMDEXMBX02.core.umdnj.edu>
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> I would be worried too.
>> Wearing gloves in the lab is always a good practice and I believe, a 
>> requirement in my lab whenever handling reagents or the possibility of 
>> coming into contact with reagents.
>> You have the right to wear gloves if you want.
>> 
>> Dana Settembre
>> University Hospital - UMDNJ
>> Newark, NJ
>> 
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu 
>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jenny 
>> Vega
>> Sent: Tuesday, February 21, 2012 10:18 PM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Does xylene cause skin cancer?
>> 
>> I am asking this because in my job we mount slides by hand, and my 
>> coworkers don't like to use gloves because it leaves a residue of 
>> latex in the back of the slides. I really don't feel comfortable 
>> mounting without gloves because I heard that xyelene can cause cancer. 
>> Some people I know personally has told me that this is not possible, 
>> but I read in some places that xylene could a possible carcinogen.
>> 
>> I have already gotten contact with xylene in my hands a couple of 
>> times and I am worried.
>> 
>> 
>> 
>> Thanks.
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>> 
>> ------------------------------
>> 
>> Message: 7
>> Date: Wed, 22 Feb 2012 12:20:02 +0000
>> From: Dennis Hahn <Dennis.Hahn <@t> cookchildrens.org>
>> Subject: [Histonet] Storage of Frozen Tissues
>> To: "'histonet <@t> lists.utsouthwestern.edu'"
>> � � � �<histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> � � � �
>> <DB0B6557A0E64E448202D8C0F86538B315ED08D6 <@t> CCHCSMBX01.CCHCS.LDAP>
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> How does everyone store tissues that are at -20 or -80? Currently we wrap
> the tissue well in foil and place in a labeled cassette. If shipped out, we
> double bag as required. Recently, a concern has been raised about the
> cassettes being a safety issue due to the fact that the tissue could be
> exposed to staff. Any ideas?
>> 
>> Thanks,
>> Dennis
>> 
>> Dennis Hahn, HT (ASCP)
>> Histology Lab Supervisor
>> Cook Children's Medical Center
>> 801 7th Avenue
>> Ft. Worth, TX 76104
>> (682) 885-6168
>> 
>> 
>> 
>> ________________________________
>> 
>> ----------------------------------------------------------------------
>> ------------------
>> Cook Children's Health Care System
>> 
>> This e-mail, facsimile, or letter and any files or attachments transmitted
> may contain information that is confidential and privileged. This
> information is intended only for the use of the individual(s) and
> entity(ies) to whom it is addressed. If you are the intended recipient,
> further disclosures are prohibited without proper authorization. If you are
> not the intended recipient, any disclosure, copying, printing, or use of
> this information is strictly prohibited and possibly a violation of federal
> or state law and regulations.
>> 
>> If you have received this information in error, please notify Cook
> Children's Health Care System immediately at (682)885-4000 or via e-mail at
> compliance <@t> cookchildrens.org. Cook Children's Health Care System, its
> subsidiaries, and affiliates hereby claim all applicable privileges related
> to this information.
>> ----------------------------------------------------------------------
>> -----------------
>> 
>> 
>> ------------------------------
>> 
>> Message: 8
>> Date: Wed, 22 Feb 2012 05:08:51 -0800 (PST)
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] Does xylene cause skin cancer?
>> To: histonet <@t> lists.utsouthwestern.edu, Jenny Vega
>> � � � �<histotech411 <@t> gmail.com>
>> Message-ID:
>> � � � �
>> <1329916131.63705.YahooMailClassic <@t> web162101.mail.bf1.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>> 
>> There is no evidence in the literature about skin cancer produced by
> xylene, although dermatitis are well documented.
>> Regardless you should use gloves whenever your hands can get in contact
> with any chemical as a good safety practice. If your colleagues do not want
> to use gloves, that is their prerogative, as is yours to wear them.
>> Ren� J.
>> 
>> --- On Tue, 2/21/12, Jenny Vega <histotech411 <@t> gmail.com> wrote:
>> 
>> 
>> From: Jenny Vega <histotech411 <@t> gmail.com>
>> Subject: [Histonet] Does xylene cause skin cancer?
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Tuesday, February 21, 2012, 10:17 PM
>> 
>> 
>> I am asking this because in my job we mount slides by hand, and my 
>> coworkers don't like to use gloves because it leaves a residue of 
>> latex in the back of the slides. I really don't feel comfortable 
>> mounting without gloves because I heard that xyelene can cause cancer. 
>> Some people I know personally has