[Histonet] IHC and negative controls?

Hoekert, W.E.J. W.E.J.Hoekert <@t> olvg.nl
Fri Feb 17 09:54:16 CST 2012

We are not using negative controls. We only add positive controls at the bottom of our patient slides. Sometimes there are 3 different types of control tissue (a TMA), so that you can use the same control block for several antibodies. You could add a negative control in the same block as the positive control and add these at the bottom of your slides (or at the top, if you wish).
Willem Hoekert
The Netherlands


Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Tom McNemar
Verzonden: do 16-2-2012 16:27
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] IHC and negative controls?

Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists no longer want us to run negatives and will use internal negatives instead.  Is this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
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