[Histonet] Cutting Tendon
Rene J Buesa
rjbuesa <@t> yahoo.com
Fri Feb 17 08:53:18 CST 2012
First of all fixation has to be perfect, if with NBF, at least 72 hours. Then increase twice your standard processing times, specially infiltration. Substitute your normal paraffin was to one of 63-65ºC and chill be blocks before sectioning.
--- On Fri, 2/17/12, Pablo Sanchez-Quinteiro <pablo.sanchez <@t> usc.es> wrote:
From: Pablo Sanchez-Quinteiro <pablo.sanchez <@t> usc.es>
Subject: [Histonet] Cutting Tendon
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 17, 2012, 6:13 AM
I am trying to get transversal sections of cow flexor digital tendons.
I need sections of the whole piece, about 1cm diameter. Before
paraffin embedding I have treated the samples with:
- KOH 4%
- 4% phenol in 70% ethanol
In both cases the results are very poor; the tissue is torn to pieces.
I would very grateful for any input regarding this issue.
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