[Histonet] Myelin staining of fresh-frozen tissue

Hilary Smith hsmith <@t> wakehealth.edu
Thu Feb 16 13:14:32 CST 2012

Hi Histonetters

Has anyone had success with Weil's myelin stain in fresh-frozen tissue?  And what is my best bet for post-fixation prior to staining?  Will 4% paraformaldehyde do the trick?  Presumably for a myelin stain I shouldn't defat?



Hilary Smith
Research Assistant
Porrino Neuroimaging Lab
Department of Physiology and Pharmacology
Wake Forest School of Medicine
Medical Center Boulevard
Winston-Salem,  NC 27157
Phone 336.716.8649
Fax 336.716.8689

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