[Histonet] RE: IHC and negative controls?

[Settembre, Dana]

Tom,
As Tunde says, the Checklist 7/11/2011 question ANP.22570 does explain that you can do as your pathologists suggests, 
BUT there are rules to be followed if that is what you will do.

"A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control:
1. Multitissue blocks ...
2. The positive control slide or the patient test slides, if these slides contain tissue elements that should not react with the antibody.
[this is the one you want to follow]
3. A separate negative tissue control slide.

The type of negative tissue control used ... SHOULD BE SPECIFIED in the laboratory manual. ...

Evidence of Compliance:
Written procedure of the selection and use of ... tissue controls for IHC..."

Dana Settembre
University Hospital - UMDNJ
Newark, NJ

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade
Sent: Thursday, February 16, 2012 10:40 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Cc: 'histonet-bounces <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: IHC and negative controls?

I am not sure if they can do that because CAP ANP.22570, explain this.

 Tunde Ajibade BS, HTL(ASCP)QIHC
 Histology Supervisor
 Medical Center Hospital
 Odessa,TX
 Tel:432-640-2348
 Fax:432-640-2303
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Thursday, February 16, 2012 9:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC and negative controls?

Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists no longer want us to run negatives and will use internal negatives instead.  Is this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org>

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