[Histonet] FATTY TISSUSE

[Rene J Buesa]

There is no "rapid solution" to fixing fatty tissue. By its own nature of being "fatty" it hampers the formalin penetration and require more time than other tissues.
If you use alcoholic-formalin, you are fixing it with ethanol and with formalin INDEPENDENTLY because each will penetrate the tissue at a different rate.
If you cut the fixation time you will end with an alcoholic fixation, reinforced with the dehydrating alcohols, but it will be lacking the formalin fixation.
Take your time and you will avoid improper infiltration consequence of an improper fixation.
René J.

--- On Mon, 2/6/12, Dawn Oakes <doakes <@t> olympicmedical.org> wrote:


From: Dawn Oakes <doakes <@t> olympicmedical.org>
Subject: [Histonet] FATTY TISSUSE
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, February 6, 2012, 1:17 PM


We would like to know what rapid fix solution is being used prior to
putting fatty tissues on the  tissue processor with alcoholic formalin.?
We do not have the availability of a separate program. Thanks ,



Dawn Oakes HT ASCP

Olympic Medical Center

939 Caroline Street 

Port Angeles, WA 98362

360-417-7467


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