[Histonet] (no subject)

Thu Dec 20 11:57:16 CST 2012

If you want to do TRAP staining for osteoclasts you will need to use 10 -15% EDTA at neutral pH to decalcify.  It will take time but using agitation and changing the solution daily will facilitate the decalcification.  
Mike

Michael Starbuck, M.S.
Laboratory Coordinator
Bone Disease Program of Texas Histomorphometry Core
GU Medical Oncology
The University of Texas M. D. Anderson Cancer Center
T7.3966
ph: 713 563-1212

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vikrant Piprode
Sent: Thursday, December 20, 2012 11:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

hi 
i want to know the best method to decalcify calvarial bone of mice for subsequent osteoclast staining and goldner's trichrome staining.
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