[Histonet] RE: thyroid processing

[Tony Henwood (SCHN)]

My recommendation is to:
1.	Fix well (at least 20% longer than equivalent sized colon blocks)
2	Process using usual times
3	After trimming blocks, soak in cold water prior to sectioning.

This should dramatically improve your sections

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Thursday, 20 December 2012 1:48 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] thyroid processing

Does anyone have some good tips to processing thyroid tissue from thyroidectomy specimens?  There seems to be a fine line between underprocessing and overprocessing where the tissue becomes brittle.  We currently process them on an overnite run where the tissue is in all reagents for 45 minutes.  We have also tried a shorter run on the Peloris that takes about 6 hours.  Would like to know what others are doing for more optimal tissue processing!!

Thanks so much everyone!

Dorothy Webb



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