[Histonet] Maximum bone sample size for methyl methacrylate embedding

[Orla M Gallagher]

Dear Histonetters,

Would anyone advise on the maximum size sample of undecalcified bone which
could be properly processed into methyl methacrylate for sectioning and
staining for Goldner's trichrome? Would anyone have a protocol for
processing large bone samples (possibly 2 x1cm) into MMA as most of the
protocols I've seen are based on Bordier trephine 5mm iliac crest biopsies.

Thank you,
Orla

-- 
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         00353114-2713337 (office)
              00353114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


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