[Histonet] Enquiry about blood removal from frozen tissue sections

[Sue Hunter]

You could try hydrogen peroxide - it wil get rid of the heme in the RBCs which is probably giving you the background.  I would try a 1:10 dilution (or higher)  of the H2O2 (in PBS) that you usually get from the store/pharmacy - undiluted will probably bubble too much and lift off the tissue.  You will have to try dilutions and time - usually 10 minutes is good, but with a diluted solution you may need to go longer.  Then wash the slides in PBS to get rid of the H2O2.
Good Luck
Sue
Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of girish cm
Sent: Tuesday, December 11, 2012 12:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Enquiry about blood removal from frozen tissue sections

Hello,

         I would like to know whether any methods (other than cold PBS
wash) or products are available to remove the blood components from frozen tissue sections. My application is antibody targeted spectroscopic imaging in which the blood components are providing background noise.

Thanking you,

Regards
--
Girish C M
Senior Research Fellow
Amrita Centre for Nanoscience & Molecular Medicine Amrita Institute of Medical Sciences & Research Centre Cochin, Kerala
India-682 041
Ph: 9645095045
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