[Histonet] Message 2: dako her2
M. Kap
m.kap.1 <@t> erasmusmc.nl
Fri Dec 7 12:09:19 CST 2012
Check www.cqpath.com for more info
Verstuurd vanaf mijn iPad
Op 7 dec. 2012 om 19:03 heeft "histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu> het volgende geschreven:
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> Today's Topics:
>
> 1. How to open Reichert 820? (Jon Krupp)
> 2. DAKO Her2 (Joanne Clark)
> 3. Embedding Centers (Tim Wheelock)
> 4. RE: Embedding Centers (O'Donnell, Bill)
> 5. Paraformaldehyde Solution Recipe for Perfusion (Andrew Coleman)
> 6. Re: automated microtomes (Jay Lundgren)
> 7. RE: Embedding Centers (Burton, Lynn)
> 8. RE: automated microtomes (Rathborne, Toni)
> 9. refurbished histology equipment (Patsy Ruegg)
> 10. Alcian Blue (Sheila Adey)
> 11. RE: Alcian Blue (Susan.Walzer <@t> HCAHealthcare.com)
> 12. RE: Alcian Blue (Lynette Pavelich)
> 13. Re: Alcian Blue (Rene J Buesa)
> 14. Re: Paraformaldehyde Solution Recipe for Perfusion (Geoff)
> 15. TISSUE PROCESSOR FOLLOW-UP QUESTION (Tim Wheelock)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 6 Dec 2012 10:10:31 -0800
> From: Jon Krupp <jkrupp <@t> deltacollege.edu>
> Subject: [Histonet] How to open Reichert 820?
> To: HISTONET <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <09FFDB2C-3CAD-4342-B6BB-4A5299D72BC8 <@t> deltacollege.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Hi
>
> Anyone know how to open the cover of a Reichert 820 microtome?
>
> This is the model that has a wheel for the coarse advance on the left side.
>
> I am used to the AO Style, pop the latch & tip it back. These usually have a crank and a cut out slot on the left.
>
> This one uses 4 screws from the bottom to secure the lid and there is no cut out for the coarse advance to slide through. Looks like the wheel has to be removed to remove the cover. Getting the wheel off is where I am stuck.
>
> This is an old microtome we have had sitting around, would like to check the guts and clean it up.
>
> Jon
>
>
> Jonathan Krupp
> Applied Science, Business & Technology
> San Joaquin Delta College
> 5151 Pacific Ave.
> Stockton, CA 95207
> 209-954-5284
> jkrupp <@t> deltacollege.edu
>
> Find us on Facebook @
> Electron Microscopy at SJ Delta College
>
>
>
>
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 6 Dec 2012 18:32:49 +0000
> From: Joanne Clark <jclark <@t> pcnm.com>
> Subject: [Histonet] DAKO Her2
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0494A7D4E8CC254EA2FB81464982E37894637A37 <@t> S10MAILD001N4.SH10.lan>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Hi All, sorry for how I am submitting this but I have been sending in questions to the address provided by nothing is going through.
> I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. We want to start running this on our Leica BOND and any info would be much appreciated.
>
> Thanks
> Joanne Clark, HT(ASCP)
> Histology Supervisor
> Pathology Consultants of New Mexico
> Roswell, NM
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 06 Dec 2012 15:28:50 -0500
> From: Tim Wheelock <twheelock <@t> mclean.harvard.edu>
> Subject: [Histonet] Embedding Centers
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <50C10002.8070708 <@t> mclean.harvard.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hi Everyone:
>
> I am also in the market for a new paraffin embedding center.
> I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and
> the Thermo-Fisher HistoStar.
> I was wondering if people could give me their critical opinion on these,
> or other machines.
> What sorts of problems have you had with them.
>
> I currently have a 25 year old Shandon Embedding Center. I like it a lot.
> But I would like to find a machine with a specimen holding tank large
> enough to allow me to immerse 300 cassettes all at once.
> This is because I infiltrate brain tissue with Tissue Path Paraplast but
> embed with Surgipath Embedding Media
> So I let the cassettes sit immersed in the Surgipath for an hour or two
> before embedding.
>
> (Until I can buy a new processor, The Shandon's holding tank also serves
> as a third processing station, since my Shandon Hypercenter has only 2
> wax reservoirs)
> I also do not feel comfortable having the cassettes sitting dry in the
> holding tank
>
> Thanks,
>
>
> Tim Wheelock
> Neuropathology Laboratory
> Harvard Brain Bank
> McLean Hospital
> Belmont, MA
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 6 Dec 2012 13:41:28 -0700
> From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
> Subject: RE: [Histonet] Embedding Centers
> To: "Tim Wheelock" <twheelock <@t> mclean.harvard.edu>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4940DF6D1C5FDF48931B6966AAEF93958EF23C <@t> chimsx08.CHI.catholichealth.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Tim - Tissue-Tek 5 has been trouble-free for the 4 years we have had it
> with one exception. Early on something went goofy with the dispensing
> mechanism. Sakura fixed it promptly and it has been great ever since. -
> Bill
>
> William (Bill) O'Donnell, HT (ASCP) QIHC
> Senior Histologist
> Good Samaritan Hospital
> 10 East 31st Street
> Kearney, NE 68847
>
> SERENITY is not freedom from the storm, but peace amid the storm.
>
> Cultivate it in PRAYER!
>
>
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim
> Wheelock
> Sent: Thursday, December 06, 2012 2:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Embedding Centers
>
> Hi Everyone:
>
> I am also in the market for a new paraffin embedding center.
> I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and
> the Thermo-Fisher HistoStar.
> I was wondering if people could give me their critical opinion on these,
> or other machines.
> What sorts of problems have you had with them.
>
> I currently have a 25 year old Shandon Embedding Center. I like it a
> lot.
> But I would like to find a machine with a specimen holding tank large
> enough to allow me to immerse 300 cassettes all at once.
> This is because I infiltrate brain tissue with Tissue Path Paraplast but
> embed with Surgipath Embedding Media So I let the cassettes sit immersed
> in the Surgipath for an hour or two before embedding.
>
> (Until I can buy a new processor, The Shandon's holding tank also serves
> as a third processing station, since my Shandon Hypercenter has only 2
> wax reservoirs) I also do not feel comfortable having the cassettes
> sitting dry in the holding tank
>
> Thanks,
>
>
> Tim Wheelock
> Neuropathology Laboratory
> Harvard Brain Bank
> McLean Hospital
> Belmont, MA
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 6 Dec 2012 15:43:09 -0500
> From: Andrew Coleman <andrewcoleman131 <@t> gmail.com>
> Subject: [Histonet] Paraformaldehyde Solution Recipe for Perfusion
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAFAJL_yVERuVpPYtbcBLZ0MgrNHeTtCgcbWJgdSXmS-_A8wN_Q <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi all,
>
> We are performing transcardial perfusions in rats using paraformaldehyde in
> 0.1M potassium phosphate buffer.
>
> Can anyone think of any issues that would be caused by using phosphate
> buffer made from solely potassium salts (basic and dibasic), rather than a
> mixture of sodium and potassium or only the sodium salts? We do our rinse
> with 0.1 M PB + Saline and then follow up with solutions just made up in
> the potassium phosphate buffer (therefore no Na+).
>
> Could this cause any tonicity/osmolarity issues? We are trying to
> troubleshoot some issues we are having with the perfused tissue.
>
> Thanks! - Andrew
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 6 Dec 2012 16:21:54 -0500
> From: Jay Lundgren <jaylundgren <@t> gmail.com>
> Subject: Re: [Histonet] automated microtomes
> To: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CANCZNuZEdKyhvCys4hiJTngHmXn_Q8sYp1NeFWPdZwkYZUdLqg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Leica is making the sweetest 'tomes out there at the moment, IMHO.
>
> Sincerely,
>
> Jay A. Lundgren, M.S., HTL
> (ASCP)
>
>
> On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich <LPaveli1 <@t> hurleymc.com>wrote:
>
>> I am purchasing my second Leica RM2255 automated microtome. Rene' is
>> correct in saying that the carpal tunnel syndrome will eventually affect
>> every advanced tech by using any microtome manually. On the RM2255, the
>> flywheel additionally is automated. You can choose to use the flywheel
>> manually or automated by the simultaneous pushing of two buttons. Nice
>> feature for us "oldies" to ease us into automation or if you have a tiny
>> specimen that you need to take extra care with and want to use manually.
>> Companies are very happy to send in a demo to try for a couple weeks.
>>
>> Happy shopping!! ;)
>> Lynette
>>
>> ________________________________________
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [
>> histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Rene J Buesa [
>> rjbuesa <@t> yahoo.com]
>> Sent: Tuesday, December 04, 2012 1:19 PM
>> To: Rathborne, Toni; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] automated microtomes
>>
>> The advantage of the so called automated microtomes (the only thing
>> automated about them is the block advance) is that they alleviate wrist
>> effort and in some ways prevent carpal tunnel syndrome that affects some
>> histotechs (mostly of the "senior persuasion").
>> I would go with the Leica.
>> Ren? J.
>>
>> From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
>> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
>> Sent: Tuesday, December 4, 2012 11:48 AM
>> Subject: [Histonet] automated microtomes
>>
>> I'm looking for some opinions about the automated microtomes currently
>> available. Which ones do most techs prefer? Which are more reliable? Is
>> there an advantage to having a semi-automated microtome?
>> Thanks in advance for your replies.
>>
>> Toni
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 6 Dec 2012 21:21:58 +0000
> From: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>
> Subject: RE: [Histonet] Embedding Centers
> To: Tim Wheelock <twheelock <@t> mclean.harvard.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <ED8BB12FF8B98347BE7A3BAE9087B546021174 <@t> il084exmbx43.illinois.gov>
> Content-Type: text/plain; charset="us-ascii"
>
> Both animal disease labs in the state of Illinois have used the Sakura machines for the twenty years I have been here with great success. We also have aSakura processor that has been going for 25 years and a coverslipper that has only had 3 service calls for minor problems in the past 15+ years. They make good products.
> Lynn Burton
> Animal Disease Lab
> Galesburg, Il
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
> Sent: Thursday, December 06, 2012 2:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Embedding Centers
>
> Hi Everyone:
>
> I am also in the market for a new paraffin embedding center.
> I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the Thermo-Fisher HistoStar.
> I was wondering if people could give me their critical opinion on these, or other machines.
> What sorts of problems have you had with them.
>
> I currently have a 25 year old Shandon Embedding Center. I like it a lot.
> But I would like to find a machine with a specimen holding tank large enough to allow me to immerse 300 cassettes all at once.
> This is because I infiltrate brain tissue with Tissue Path Paraplast but embed with Surgipath Embedding Media So I let the cassettes sit immersed in the Surgipath for an hour or two before embedding.
>
> (Until I can buy a new processor, The Shandon's holding tank also serves as a third processing station, since my Shandon Hypercenter has only 2 wax reservoirs) I also do not feel comfortable having the cassettes sitting dry in the holding tank
>
> Thanks,
>
>
> Tim Wheelock
> Neuropathology Laboratory
> Harvard Brain Bank
> McLean Hospital
> Belmont, MA
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 6 Dec 2012 21:47:18 +0000
> From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
> Subject: RE: [Histonet] automated microtomes
> To: "'Jay Lundgren'" <jaylundgren <@t> gmail.com>, Lynette Pavelich
> <LPaveli1 <@t> hurleymc.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3AD061FE740D464FAC7BF6B5CFB757071F1F6772 <@t> smcmail02.somerset-healthcare.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thanks all for your valued opinions. I have contacted Leica, and am now waiting for a demo!
>
> From: Jay Lundgren [mailto:jaylundgren <@t> gmail.com]
> Sent: Thursday, December 06, 2012 4:22 PM
> To: Lynette Pavelich
> Cc: Rene J Buesa; Rathborne, Toni; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] automated microtomes
>
> Leica is making the sweetest 'tomes out there at the moment, IMHO.
>
> Sincerely,
>
> Jay A. Lundgren, M.S., HTL (ASCP)
>
> On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich <LPaveli1 <@t> hurleymc.com<mailto:LPaveli1 <@t> hurleymc.com>> wrote:
> I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually.
> Companies are very happy to send in a demo to try for a couple weeks.
>
> Happy shopping!! ;)
> Lynette
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu> [histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu>] on behalf of Rene J Buesa [rjbuesa <@t> yahoo.com<mailto:rjbuesa <@t> yahoo.com>]
> Sent: Tuesday, December 04, 2012 1:19 PM
> To: Rathborne, Toni; histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
> Subject: Re: [Histonet] automated microtomes
>
> The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion").
> I would go with the Leica.
> Ren? J.
>
> From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com<mailto:trathborne <@t> somerset-healthcare.com>>
> To: "histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>
> Sent: Tuesday, December 4, 2012 11:48 AM
> Subject: [Histonet] automated microtomes
>
> I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome?
> Thanks in advance for your replies.
>
> Toni
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 6 Dec 2012 15:40:47 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: [Histonet] refurbished histology equipment
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <311FA59E257C47B99E7C538F1DB384AE <@t> DESKTOP3>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone have a favorite vendor they would recommend to me? I am mostly
> looking for cryostats right now, but interested in other things, tissue
> processors, IHC stainers, etc.
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> Ruegg IHC Consulting, LLC
>
> 40864 Arkansas Ave
>
> Bennett, CO 80102
>
> Phone: 303-644-4538
>
> Fax: 720-859-4110
>
> <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Thu, 6 Dec 2012 20:58:10 -0500
> From: Sheila Adey <sadey <@t> hotmail.ca>
> Subject: [Histonet] Alcian Blue
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY154-W12F429F13129F56A6B76C5C6440 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Hi Everyone:
> Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
> Thanks
> Sheila
>
> ------------------------------
>
> Message: 11
> Date: Fri, 7 Dec 2012 02:04:37 -0600
> From: <Susan.Walzer <@t> HCAHealthcare.com>
> Subject: RE: [Histonet] Alcian Blue
> To: <sadey <@t> hotmail.ca>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4BF03F5404EBDE409AF9232DA74B9DED2DF8521C13 <@t> FWDCWPMSGCMS09.hca.corpad.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We've always used 30 minutes and it has never failed.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Adey
> Sent: Thursday, December 06, 2012 8:58 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Alcian Blue
>
>
> Hi Everyone:
> Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
> Thanks
> Sheila _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 7 Dec 2012 12:06:15 +0000
> From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
> Subject: RE: [Histonet] Alcian Blue
> To: "susan.walzer <@t> hcahealthcare.com" <susan.walzer <@t> hcahealthcare.com>,
> "sadey <@t> hotmail.ca" <sadey <@t> hotmail.ca>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <89F4666A496DC949A819ECC40E11C867BF56C8FB <@t> EXCHANGEMB1.hmc.hurleymc.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> 30 here.....
>
> Lynette
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Susan.Walzer <@t> HCAHealthcare.com [Susan.Walzer <@t> HCAHealthcare.com]
> Sent: Friday, December 07, 2012 3:04 AM
> To: sadey <@t> hotmail.ca; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Alcian Blue
>
> We've always used 30 minutes and it has never failed.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Adey
> Sent: Thursday, December 06, 2012 8:58 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Alcian Blue
>
>
> Hi Everyone:
> Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
> Thanks
> Sheila _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 13
> Date: Fri, 7 Dec 2012 06:11:50 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Alcian Blue
> To: Sheila Adey <sadey <@t> hotmail.ca>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1354889510.81416.YahooMailNeo <@t> web163105.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> If after many successful runs with your Alcian Blue reagents you get a complaint about either strength of staining or cells not staining at all, most likely you have a problem with the solutions. If you prepare them "in house" check the preparation date and prepare a fresh solution. The general procedure calls for 30 min, unless you use a microwave oven version of the staining protocol.
> Ren? J.
>
> From: Sheila Adey <sadey <@t> hotmail.ca>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, December 6, 2012 8:58 PM
> Subject: [Histonet] Alcian Blue
>
>
> Hi Everyone:
> Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
> Thanks
> Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> ------------------------------
>
> Message: 14
> Date: Fri, 07 Dec 2012 10:50:09 -0500
> From: Geoff <mcauliff <@t> umdnj.edu>
> Subject: Re: [Histonet] Paraformaldehyde Solution Recipe for Perfusion
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <50C21031.7060405 <@t> umdnj.edu>
> Content-Type: text/plain; CHARSET=US-ASCII; format=flowed
>
> What issues are you having? That might help the list diagnose the problem.
>
> Geoff
>
> On 12/6/2012 3:43 PM, Andrew Coleman wrote:
>> Hi all,
>>
>> We are performing transcardial perfusions in rats using paraformaldehyde in
>> 0.1M potassium phosphate buffer.
>>
>> Can anyone think of any issues that would be caused by using phosphate
>> buffer made from solely potassium salts (basic and dibasic), rather than a
>> mixture of sodium and potassium or only the sodium salts? We do our rinse
>> with 0.1 M PB + Saline and then follow up with solutions just made up in
>> the potassium phosphate buffer (therefore no Na+).
>>
>> Could this cause any tonicity/osmolarity issues? We are trying to
>> troubleshoot some issues we are having with the perfused tissue.
>>
>> Thanks! - Andrew
>> _______________________________________________
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>
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff <@t> umdnj.edu
> **********************************************
>
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 07 Dec 2012 12:44:11 -0500
> From: Tim Wheelock <twheelock <@t> mclean.harvard.edu>
> Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <50C22AEB.6070508 <@t> mclean.harvard.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Hi All:
>
> First, thank you for all your feedback on the processors.
>
> I site-visited a VIP6 at a local hospital
> From my understanding the VIP6 can rotate absolute alcohol and xylene
> (using the bulk reservoirs), as well as the paraffin stations, but it
> cannot rotate other concentrations of alcohol based on hydrometer readings.
> Am I correct in this appraisal?
>
> Tim Wheelock
> Neuropathology Laboratory
> Harvard Brain Bank
> McLean Hospital
> Belmont, MA
>
>
>
> ------------------------------
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> End of Histonet Digest, Vol 109, Issue 9
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