[Histonet] Alcian Blue

[Susan.Walzer <@t> HCAHealthcare.com]

We've always used 30 minutes and it has never failed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Adey
Sent: Thursday, December 06, 2012 8:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Alcian Blue


Hi Everyone:
Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best?
Thanks
Sheila 		 	   		  _______________________________________________
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