[Histonet] Tissue Processors
Michelle Lamphere
MICHELLE.LAMPHERE <@t> childrens.com
Wed Dec 5 14:34:50 CST 2012
We currently have three Excelsiors and I would have a hard time going back to any other processor. I really appreciate the reagent management of this machine. We have virtually eliminated all hazardous reagent exposure during reagent exchange. I think their customer service is outstanding as well as their technical support and training. I have used the VIPs and the Leica ASP 300.
The only downside that I have experienced with the Excelsior is that all three of our machines have a software glitch that will make the end date of a processing schedule get "stuck" on a previous date if the machine is run for a few days in a row and then taken off service for a few days. If you are not careful, a delayed start becomes an immediate start as the machine tries to backtrack to the previous date.
Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX 75235
Office :214-456-2798
Histology: 214-456-2318
Fax: 214-456-0779
-----Original Message-----
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Sent: Wednesday, December 05, 2012 12:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 109, Issue 6
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Today's Topics:
1. RE: automated microtomes (Lynette Pavelich)
2. HistoTALK (David Kemler)
3. RE: Tissue Processors (Marcum, Pamela A)
4. RE: Tissue Processors (Boyd, Debbie M)
5. Re: Immunohistochemical detection of cytokines in older
frozen sections (Rob Day)
6. RE: Histonet Digest, Vol 109, Issue 5 (Riesen, Rebecca)
7. Re: Fixation time (Geoff)
8. Histology Positions in Little Rock AR (Marcum, Pamela A)
9. Re: Fixation time (Teri Johnson)
10. Re: Tissue Processors (Teri Johnson)
11. job opening in PA (Hutton, Allison)
12. Re: Re: Tissue Processors (Rene J Buesa)
13. RE: Re: Tissue Processors (Ellenburg, Deborah)
----------------------------------------------------------------------
Message: 1
Date: Wed, 5 Dec 2012 12:10:06 +0000
From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
Subject: RE: [Histonet] automated microtomes
To: Rene J Buesa <rjbuesa <@t> yahoo.com>, "Rathborne, Toni"
<trathborne <@t> somerset-healthcare.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<89F4666A496DC949A819ECC40E11C867BF56C4F0 <@t> EXCHANGEMB1.hmc.hurleymc.com>
Content-Type: text/plain; charset="iso-8859-1"
I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us "oldies" to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually.
Companies are very happy to send in a demo to try for a couple weeks.
Happy shopping!! ;)
Lynette
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbuesa <@t> yahoo.com]
Sent: Tuesday, December 04, 2012 1:19 PM
To: Rathborne, Toni; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] automated microtomes
The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the "senior persuasion").
I would go with the Leica.
Ren? J.
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, December 4, 2012 11:48 AM
Subject: [Histonet] automated microtomes
I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome?
Thanks in advance for your replies.
Toni
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------------------------------
Message: 2
Date: Wed, 5 Dec 2012 04:28:54 -0800 (PST)
From: David Kemler <histotalk <@t> yahoo.com>
Subject: [Histonet] HistoTALK
To: Fellow HistoNetters <Histonet <@t> Lists.UTSouthwestern.edu>
Message-ID:
<1354710534.83756.YahooMailNeo <@t> web121504.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello HistoNetters -
?
In case you missed our last two guests on HistoTALK http://www.histotalk.com/, they were Region VII Director, ANDI GRANTHAM and KATHERINE (Kaspar)?KASPRZYK, President of the Arizona Society for Histotechnology. Just wanted to let you know.
?
Yours,
David
------------------------------
Message: 3
Date: Wed, 5 Dec 2012 13:00:57 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: RE: [Histonet] Tissue Processors
To: "'Susan.Walzer <@t> HCAHealthcare.com'"
<Susan.Walzer <@t> HCAHealthcare.com>, "rjbuesa <@t> yahoo.com"
<rjbuesa <@t> yahoo.com>, "twheelock <@t> mclean.harvard.edu"
<twheelock <@t> mclean.harvard.edu>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<41D3A1AF6FEF0643BDC89E0516A6EA328E5D5138 <@t> Mail2Node2.ad.uams.edu>
Content-Type: text/plain; charset="iso-8859-1"
We have 4 Thermo Excelsiors and prefer them to the VIP we no longer use. It is ease of changing reagents and programming as well as working better for our tissues. We also have Leica ASP300 that is used for only one type of tissue so we no longer need to change it weekly and expose personnel to the fumes of the reagents and possible spills. VIP are great units just not as easy to work with not that other options have become available.
Pam Marcum
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Susan.Walzer <@t> HCAHealthcare.com
Sent: Wednesday, December 05, 2012 1:49 AM
To: rjbuesa <@t> yahoo.com; twheelock <@t> mclean.harvard.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Tissue Processors
VIP always!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, December 04, 2012 3:57 PM
To: Tim Wheelock; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue Processors
I always used VIP because of reliability, "toughness" and customer service.
Ren? J.
From: Tim Wheelock <twheelock <@t> mclean.harvard.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, December 4, 2012 3:48 PM
Subject: [Histonet] Tissue Processors
Hi Everyone:
I am currently evaluating three tissue processors.
They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES.
I was wondering if people could give me their critical opinions and preferences on these three machines.
In addition to reliability and ease of use,? I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision.
I currently have a 14 year old Shandon Hypercenter XP.
Thank you,
Tim Wheelock
Neuropathology Laboratory
Harvard Brain Bank
McLean Hospital
Belmont, MA
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Message: 4
Date: Wed, 5 Dec 2012 13:35:29 +0000
From: "Boyd, Debbie M" <DKBoyd <@t> chs.net>
Subject: RE: [Histonet] Tissue Processors
To: Tim Wheelock <twheelock <@t> mclean.harvard.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<7EAFE982E328304DA6CE2B677BB762467949FE2A <@t> TN001WEXMBX11.US.chs.net>
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Excelsior> Great workhorse. Very little to no down time. Less tech time needed as reagents dump and rotate automatically. User friendly.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Tuesday, December 04, 2012 3:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tissue Processors
Hi Everyone:
I am currently evaluating three tissue processors.
They are the Sakura VIP6, the Leica ASP 6025, and the Thermo-Fisher Excelsior ES.
I was wondering if people could give me their critical opinions and preferences on these three machines.
In addition to reliability and ease of use, I am interested in people's experience with tech support, software, or any other factor-positive or negative-that prompted your decision.
I currently have a 14 year old Shandon Hypercenter XP.
Thank you,
Tim Wheelock
Neuropathology Laboratory
Harvard Brain Bank
McLean Hospital
Belmont, MA
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Message: 5
Date: Wed, 5 Dec 2012 08:44:45 -0500
From: Rob Day <rob <@t> foliobio.com>
Subject: Re: [Histonet] Immunohistochemical detection of cytokines in
older frozen sections
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <EEF5CA71-8195-4607-96C2-75893A3A0078 <@t> foliobio.com>
Content-Type: text/plain; charset=iso-8859-1
Folio biosciences is testing a process that will rejuvenate older sections to restore degraded antigenicity for ISH. It's designed to help scientists with exactly the problem you are describing
Email me if you want to help us test this process using your slides. No charge of course.
Rob Day.
On Dec 4, 2012, at 6:26 PM, Darius Alex Schneider <schndar <@t> gmail.com> wrote:
> Dear all,
>
> I am trying to detect cytokines in human pancreatic sections from
> diabetics. The blocks are at least 1y old, the sections are usually fresh.
> I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin,
> staining overnight in BSS-saponin with the primary, biotinylated antibody
> and subsequently tyramid signal amplification and fluorescein based
> visuatlisation. However, my results are very poor.
> I am mainly interested in these cytokines:
> IL1beta
> IL1r
> IL2
> IL6
> IL10
> IL17
> IL21
> TNFalpha
> IFNgamma
>
> Is there somebody out there who is experienced in detecting these
> cytokines, and could provide me with a comprehensive protocol? Also, who
> knows what the best positive controls might be?
>
> Thank you so much,
>
> Darius
> La Jolla, USA
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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Rob Day
Business Development
Folio Biosciences
1476 Manning Pkwy, Powell, Ohio 43065
Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815
http://foliobio.com
www.linkedin.com/in/robdaybiotech
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------------------------------
Message: 6
Date: Wed, 5 Dec 2012 13:47:44 +0000
From: "Riesen, Rebecca" <Rebecca.Riesen <@t> hma.com>
Subject: [Histonet] RE: Histonet Digest, Vol 109, Issue 5
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DF15ACC900DDF741870C8DDCD3C9159A273CA54F <@t> HMAMBX02.hma.com>
Content-Type: text/plain; charset="us-ascii"
I am in love with the Excelsior from the standpoint of hands off daily maintenance. Waste paraffin is pumped into a container with a liner, you let it solidify and remove waste in the bag for easy disposal. Pour a couple bags of paraffin pellets in and walk away. Take a bottle of waste alcohol or xylene the instrument has pumped into off and replace it with a new bottle. Excelsior will aspirate the new fluid on the next run. You don't even have to pour into another container. The Excelsior takes the fluids right out of the manufacturer's bottle. It is so simple! If you can read, that's all you need. The screen prompts everything. I also find my solutions last longer.
------------------------------
Message: 18
Date: Tue, 4 Dec 2012 15:39:11 -0800
From: Davide Costanzo <pathlocums <@t> gmail.com>
Subject: Re: [Histonet] Tissue Processors
To: Tim Wheelock <twheelock <@t> mclean.harvard.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1628083238193856494 <@t> unknownmsgid>
Content-Type: text/plain; charset=ISO-8859-1
The excelsior is getting rave reviews from folks I know that use it.
The University of Miami just ordered a few of them from my
understanding. Might want to call there and ask their opinion.
Personally, I think Sakura is losing ground and for good reason. We
use exclusively Thermo branded equipment here. STP420 and Pathcentre.
Both are great.
------------------------------
Message: 7
Date: Wed, 05 Dec 2012 09:51:53 -0500
From: Geoff <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Fixation time
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <50BF5F89.20200 <@t> umdnj.edu>
Content-Type: text/plain; CHARSET=US-ASCII; format=flowed
I agree with Rene.
Geoff
On 12/3/2012 12:00 AM, Daniela Bodemer wrote:
> Hi all,
>
>
>
> Our tissue processor has been shut down due to a contamination issue and
> now all the tissues (mice and rat pelves) collected prior to this
> happening have been sitting in 4% PFA. Some tissues more than a week,
> when we usually fix for 48 hours.
>
> Now we are transferring the tissues to 70% Ethanol and they will sit
> there until further notice. I am concerned about this process and what
> it will do to the tissue and would like your thoughts on this.
>
>
>
> Many thanks,
>
>
>
> DB
>
> Research Assistant
>
>
>
>
>
>
> ______________________________________________________________________
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 8
Date: Wed, 5 Dec 2012 16:09:20 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: [Histonet] Histology Positions in Little Rock AR
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<41D3A1AF6FEF0643BDC89E0516A6EA32924B5C23 <@t> Mail2Node2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming.
Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.)
Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO)
Best Regards,
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Message: 9
Date: Wed, 5 Dec 2012 16:32:43 +0000
From: Teri Johnson <TJohnson <@t> gnf.org>
Subject: [Histonet] Re: Fixation time
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9F3CFEE76E51B64991C7485270890B400CE128F3 <@t> EX4.lj.gnf.org>
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Hi Daniela,
We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that could introduce variation in your experimental outcome. Be very critical of the staining results you obtain on these samples compared to your others.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
------------------------------
Message: 10
Date: Wed, 5 Dec 2012 16:37:56 +0000
From: Teri Johnson <TJohnson <@t> gnf.org>
Subject: [Histonet] Re: Tissue Processors
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9F3CFEE76E51B64991C7485270890B400CE12902 <@t> EX4.lj.gnf.org>
Content-Type: text/plain; charset="us-ascii"
Dear Tim,
Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
------------------------------
Message: 11
Date: Wed, 5 Dec 2012 11:42:21 -0500
From: "Hutton, Allison" <AHutton <@t> dh.org>
Subject: [Histonet] job opening in PA
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<38A56C4F4630D348A50B3720409270870E0FE6A1 <@t> dhmail.dhorg.org>
Content-Type: text/plain; charset="iso-8859-1"
> Doylestown Hospital is Doylestown, PA has the following position open:
>
> Job Title: Histology Tech - 1123
> Req: 2012-2409
> Location:
> Department: Laboratory
> Schedule: Mon-Fri
> varied start times 0530,0630,0730
>
> Job Description:
> PURPOSE OF JOB: To assist the Histology and cytology Department in the preparation of tissue and cells for microscope pathology. Performs Histologic and Cytologic procedures as states in the Histology and Cytology Procedure Manuals. ESSENTIAL FUNCTIONS: 45% 1. Properly orients, embeds and sections tissues at 5 microns without artifacts or contaminants. 25% 2. Performs routine, special, frozen and immunohistochemical staining. Manually prepares all stock and working solutions needed to perform above stains (Stain list is available in Histology). 20% 3. Accurately and legibly accessions specimens with the use of the computer. Assists pathologist with frozen sections. Follows slides through the H&E stainer and appropriately distributes slides to the pathologists. 5% 4. Operates and maintains all histology equipment, recycles chemicals and disposes of pathological waste, troubleshoots outliers to help keep department running efficiently. 5% 5. Covers the Cytology Department in the absence of the cytotechnologist. Assists Cytology and Office with designated duties.
> For more information, please visit www.dh.org
>
>
>
------------------------------
Message: 12
Date: Wed, 5 Dec 2012 08:54:40 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Re: Tissue Processors
To: Teri Johnson <TJohnson <@t> gnf.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1354726480.27777.YahooMailNeo <@t> web163106.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=utf-8
If I may, I would like to add something:
do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat.
No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it.
For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers).
Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable.
Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong".
Regarding lower exposure to reagents, that depends on the reagents, not on the instrument.
It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health.
This is my humble opinion.
Ren?? J.
From: Teri Johnson <TJohnson <@t> gnf.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, December 5, 2012 11:37 AM
Subject: [Histonet] Re: Tissue Processors
Dear Tim,
Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 13
Date: Wed, 5 Dec 2012 12:13:51 -0500
From: "Ellenburg, Deborah" <DEBORAH_ELLENBURG <@t> bshsi.org>
Subject: RE: [Histonet] Re: Tissue Processors
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Teri Johnson"
<TJohnson <@t> gnf.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BB153A828AB3FA439FBD55C818A0EC8801A46D81 <@t> EDC-MAIL-02.ads.bshsi.com>
Content-Type: text/plain; charset="UTF-8"
Well stated - agree 100%.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, December 05, 2012 11:55 AM
To: Teri Johnson; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Tissue Processors
If I may, I would like to add something:
do you remember the sales success of the VW Beetle? Why was it a success? Because of its simplicity. They did not even have a gasoline gage and if you ran out of gasoline there was 1 extra gallon reserve that you opened with a handle underneath the passenger's seat.
No thrills, no complexity. You could repair it yourself. Try to do now with a new VW "Beetle", the new ones with the motor at the front of the car. You will not be able to do it.
For the same reason there are labs that today still use the "carousel" type tissue processors, the "Technicon" type still manufactured (now by Leica and some Indian and Chinese lab manufacturers).
Something similar can be said to the "basic" VIP with less valves, less automation but extremely reliable.
Those more "automated" with "hands-free reagents transfer" by laws of probability have to be more prone to failure. More components = greater parts to "go wrong".
Regarding lower exposure to reagents, that depends on the reagents, not on the instrument.
It is well documented at this moment that the only reagents you need to process tissue and obtain the best quality possible is with innocuous reagents??in a protocol using??isopropyl alcohol ??? mineral oil ??? paraffin??neither are noxious to the techs' health.
This is my humble opinion.
Ren?? J.
From: Teri Johnson <TJohnson <@t> gnf.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, December 5, 2012 11:37 AM
Subject: [Histonet] Re: Tissue Processors
Dear Tim,
Here's the deal, they all work. When buying new, I bought the VIP5 due to its great reputation. It was and still is a workhorse.
Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752
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End of Histonet Digest, Vol 109, Issue 6
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