[Histonet] Re: Fixation time

[Teri Johnson]

Hi Daniela,

We have successfully stored in 70% ethanol without issue. The only published account I have seen of any issue with alcohol storage is it causes vacuolization in brain. I would not really want to store in fixative any longer because you will continue creating cross links and that could introduce variation in your experimental outcome. Be very critical of the staining results you obtain on these samples compared to your others.

Teri Johnson, HT(ASCP)QIHC
GNF Histology Lab Manager
Genomics Institute of the Novartis Research Foundation
858-332-4752