[Histonet] Osmium tetroxide staining for lipids

[Geoff McAuliffe]

Two other arguments against osmium, it is very expensive and is must be 
disposed of as hazardous waste.

Geoff

On 8/24/2012 10:50 AM, Sheila Adey wrote:
> Thank you so much for this response. :)
>   
>
>> Subject: RE: [Histonet] Osmium tetroxide staining for lipids
>> Date: Fri, 24 Aug 2012 08:42:34 -0600
>> From: billodonnell <@t> catholichealth.net
>> To: rjbuesa <@t> yahoo.com; sadey <@t> hotmail.ca; histonet <@t> lists.utsouthwestern.edu
>>
>> I agree w Rene on this one - do not use this stuff if you do not have to - and in 2012 (or at least in the last 20 years or more) - you do not HAVE to! - Oil Red O is far safer - Bill
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
>> Sent: Friday, August 24, 2012 9:32 AM
>> To: Sheila Adey; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Osmium tetroxide staining for lipids
>>
>> Osmium tetroxide is one of the most dangerous substances you can use in the laboratory. Your pathologist probably read some article or found an old photo of fat "stained" with osmium tetroxide and now wants you to do the same thing.
>> The problem is that the fat is allowed to react to the fumes of this very nasty substance and this is a very dangerous step.
>> Nowadays this is never done. If he wants to "demonstrate" fat, freeze the tissue, prepare a frozen section and us Oil Red to demonstrate fat.
>> This is the most current measure.
>> On the other hand, as you point out, osmium tetroxide is used in electron microscopy.
>> René J.
>>
>>
>> ________________________________
>> From: Sheila Adey <sadey <@t> hotmail.ca>
>> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Friday, August 24, 2012 8:30 AM
>> Subject: [Histonet] Osmium tetroxide staining for lipids
>>
>>
>> Hi Everyone:
>>
>> One of my pathologists wants me to look into Osmium Tetroxide for staining lipids. From what I can gather on the internet, it looks like it is used in Electron microscopy for fixation and staining.
>> Is anyone using this procedure for routine 4 micrometer sections?
>>
>> Thanks
>> :)
>> Sheila                         _______________________________________________
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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