[Histonet] DAB precipitate on H. pylori

[Susan.Walzer <@t> HCAHealthcare.com]

We never had a problem until they changed AB's on us. We sent them slides procedures etc to test but in the mean time we tried Cell Marques AB and it was clean. Problem solved. Now we all need to demand that Ventana switch back to Cell Marques H pylori so that we do not have to pay for those #@*&% prep kits.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa Perez
Sent: Monday, August 20, 2012 2:11 PM
To: Dorothy Glass; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] DAB precipitate on H. pylori

Are you using the ventana h. pylori?  If you are we are having that problem with some of our satellite labs.  Ventana said its an issue with that new antibody and some labs but not all are experiencing this.  Have you called it in the Customer support???

Vanessa Perez Garcia
Histology Supervisor
Pathology Reference Lab
210-892-3746
210-892-3732
vperez <@t> pathreflab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dorothy Glass
Sent: Monday, August 20, 2012 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DAB precipitate on H. pylori

I have been getting something on some slides, not all that looks like bacteria but it is not. It looks like Dab precipitate. It looks like the Dab is breaking down,  but only noticeable on the stains for H. pylori.  I wash my water bath, and changes my blades. It not occurs all the time, but it has happened about four times.  Could it be the water not being filtered properly. We have a water filtering system, but it has not been working well lately. Would that have anything to do with the brown patches on H. pylori?
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