[Histonet] RE: Histonet Digest, Vol 105, Issue 20

Fri Aug 17 10:24:41 CDT 2012

Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free). 

Respectfully,
Pamela Romundstad HT, QIHC

Gundersen Lutheran

608-775-3139

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Thursday, August 16, 2012 12:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 105, Issue 20

Send Histonet mailing list submissions to
        histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
        histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
        histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."

Today's Topics:

   1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline)
   2. Re: Two identifiers... (Bob Richmond)
   3. RE: Re: Two identifiers... (joelle weaver)
   4. RE: Re: Two identifiers... (Michael Mihalik)
   5. Negative Control (Pardue, Judith)
   6. RE: Special Stain documentation (hymclab)
   7. Peloris (Pardue, Judith)
   8. RE: RE: Two identifiers... (Pratt, Caroline)
   9. RE: RE: Two identifiers... (Tom McNemar)
  10. marker specific  for mouse fibroblasts (Kim Merriam)
  11. RE: RE: Two identifiers... (Pratt, Caroline)
  12. RE: marker specific  for mouse fibroblasts (Elizabeth Chlipala)

----------------------------------------------------------------------

Message: 1
Date: Wed, 15 Aug 2012 18:07:46 -0400
From: "Bass, Caroline" <cebass <@t> buffalo.edu>
Subject: [Histonet] protocol for EGFP IHC in frozen rat brains
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <ABC535EC-9FCD-45E0-957C-617FA7AA8B53 <@t> buffalo.edu>
Content-Type: text/plain; charset="Windows-1252"

Hello Everyone,

I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions?

1) what thickness should I cut, 50 um works great for my fixed sections.
2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration?
3) could I then proceed as though this were a normal GFP immuno, with DAB stain?
4) should I used plus slides or subbed slides?
5) will the sections stay on without fixation?

Any and all suggestions would be appreciated!

Thanks,

Caroline

------------------------------

Message: 2
Date: Wed, 15 Aug 2012 21:11:12 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Two identifiers...
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAOKsRH5bHaSnoncK7Zg+04QjD23O0nfo+5T-8FjL71hS6afggQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

If a pathologist end-user may make an observation here:

When I pick up a slide and look to match it with the paperwork in
front of me, it's a great help if the patient's name is on the slide,
since names are always easier to read than numbers. Having the
patient's name on the slide means I'm less likely to mix up two cases.
I can't do that with a bar code or a second essentially meaningless
number.

This may however be a situation where good patient care must take a
back seat to good management practice and good regulator compliance.

Bob Richmond
Samurai Pathologist
Maryville TN

------------------------------

Message: 3
Date: Thu, 16 Aug 2012 05:24:56 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] Re: Two identifiers...
To: <rsrichmond <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <SNT135-W523C7297BE0A6C24B5B44BD8B50 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill?  I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone.  I realize barcodes are not helpful to people doing handling unfortunately, but they do  help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious,  and allow tracking for workflow- but many people still prefer human-manual labeling methods.

Joelle Weaver MAOM, HTL (ASCP) QIHC
 > Date: Wed, 15 Aug 2012 21:11:12 -0400
> From: rsrichmond <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Two identifiers...
>
> If a pathologist end-user may make an observation here:
>
> When I pick up a slide and look to match it with the paperwork in
> front of me, it's a great help if the patient's name is on the slide,
> since names are always easier to read than numbers. Having the
> patient's name on the slide means I'm less likely to mix up two cases.
> I can't do that with a bar code or a second essentially meaningless
> number.
>
> This may however be a situation where good patient care must take a
> back seat to good management practice and good regulator compliance.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 4
Date: Thu, 16 Aug 2012 05:04:17 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Re: Two identifiers...
To: "'Bob Richmond'" <rsrichmond <@t> gmail.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com>
Content-Type: text/plain;       charset="iso-8859-1"

Bob, I absolutely concur with your thoughts, but in the LIS we offer, the
moment you scan the slide, the 'paperwork' displays on the screen
automatically, effectively negating the need to match paperwork with the
slide.

We also always recommend BIG monitors  or even dual monitors to make it
easier to see.  Imagine the paperwork on one monitor and a 'work' screen on
the other monitor.

Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, August 15, 2012 9:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Two identifiers...

If a pathologist end-user may make an observation here:

When I pick up a slide and look to match it with the paperwork in front of
me, it's a great help if the patient's name is on the slide, since names are
always easier to read than numbers. Having the patient's name on the slide
means I'm less likely to mix up two cases.
I can't do that with a bar code or a second essentially meaningless number.

This may however be a situation where good patient care must take a back
seat to good management practice and good regulator compliance.

Bob Richmond
Samurai Pathologist
Maryville TN

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Thu, 16 Aug 2012 04:49:50 -0600
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Negative Control
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <14B823F24E628E49BBFAD704E4BAB89ABE9BCD <@t> chimsx03.CHI.catholichealth.net>

Content-Type: text/plain;       charset="us-ascii"

At the first of August Richard Cartun posted that CAP had a new negative
control policy. Does anyone know if it is already in effect and if it
would apply to up coming inspections.

Judith Pardue

Histology Supervisor

Memorial Hospital

Chattanooga, Tn.

Judith_Pardue <@t> memorial.org

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.

------------------------------

Message: 6
Date: Thu, 16 Aug 2012 07:51:41 -0500
From: hymclab <hymclab.hymclab <@t> ministryhealth.org>
Subject: RE: [Histonet] Special Stain documentation
To: "'Weems, Joyce K.'" <joyce.weems <@t> emoryhealthcare.org>,
        "'laurie <@t> blufrogpath.com'" <laurie <@t> blufrogpath.com>, Histonet post
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <EF3001233998854390C692D19B058F0F70FECA4B60 <@t> EXMHCMBX01VS.ministryhealth.net>

Content-Type: text/plain; charset="us-ascii"

For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials.  The lead tech also initials.

For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains.  The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report.

Dawn

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, August 13, 2012 5:06 PM
To: 'Laurie <@t> blufrogpath.com'; Histonet post
Subject: RE: [Histonet] Special Stain documentation

Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately".

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org

www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie <@t> blufrogpath.com
Sent: Monday, August 13, 2012 5:35 PM
To: Histonet post
Subject: [Histonet] Special Stain documentation

How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP  checklist item ANP.21395)?  Do the histotechs document the results or do the pathologists - or both??  If your pathologists document the results, how do they document them?

Thanks,
Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.

If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).

CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation.

------------------------------

Message: 7
Date: Thu, 16 Aug 2012 07:17:20 -0600
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Peloris
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <14B823F24E628E49BBFAD704E4BAB89ABE9C2F <@t> chimsx03.CHI.catholichealth.net>

Content-Type: text/plain;       charset="us-ascii"

Has anyone had problems with Peloris processors shuting down after a
power outage.  Both our processors shut down when the power went off
last night and they were connected to a USP and

plugged into an emergency outlet. This is the third time this has
happened and we have had to have a service tech from Leica come out.

Judith Pardue

Histology Supervisor

Memorial Hospital

Judith_Pardue <@t> memorial.org

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.

------------------------------

Message: 8
Date: Thu, 16 Aug 2012 09:46:54 -0400
From: "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu>
Subject: RE: [Histonet] RE: Two identifiers...
To: "Ron" <pathrm35 <@t> comcast.net>, <pamarcum <@t> uams.edu>,
        <tmcnemar <@t> lmhealth.org>,        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <EFA78D6D81C0F348B8FA2880AE376CC346E717 <@t> uphmasphi015.UPHS.PENNHEALTH.PRV>

Content-Type: text/plain;       charset="utf-8"

It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey.  We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number.  It is a tremendous ongoing effort, but we are there now.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ron
Sent: Wednesday, August 15, 2012 12:39 PM
To: pamarcum <@t> uams.edu; tmcnemar <@t> lmhealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Two identifiers...

We do the same as Pam with the patient name and case number

Sent from my Verizon Wireless 4G LTE smartphone

"Marcum, Pamela A" <PAMarcum <@t> uams.edu> wrote:

>We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria.  We use the Thermo cassette writer and it transfers the information to the slides.
>
>Pam Marcum
>UAMS
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
>McNemar
>Sent: Wednesday, August 15, 2012 10:03 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Two identifiers...
>
>Hello all,
>
>For those of you using cassette and slide labelers....
>Does your system permit you to print the accession number, patient name, and  DOB on the face of the cassette?  Are you using the specimen number and name as your two identifiers?
>
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Health Systems
>(740) 348-4163
>(740) 348-4166
>tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
>www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Applica
>tion%20Data\Microsoft\Signatures\www.LMHealth.org>
>
>________________________________
>This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>Confidentiality Notice: This e-mail message, including any attachments,
>is for the sole use of the intended recipient(s) and may contain
>confidential and privileged information.  Any unauthorized review, use,
>disclosure or distribution is prohibited.  If you are not the intended
>recipient, please contact the sender by reply e-mail and destroy all
>copies of the original message.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.

------------------------------

Message: 9
Date: Thu, 16 Aug 2012 09:50:45 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] RE: Two identifiers...
To: "'Pratt, Caroline'" <Caroline.Pratt <@t> uphs.upenn.edu>, Ron
        <pathrm35 <@t> comcast.net>, "pamarcum <@t> uams.edu" <pamarcum <@t> uams.edu>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E9A90E28259D2F4E84308C5E8EA8F7B4011F577646E5 <@t> lmhs-exchange.lmhealth.org>

Content-Type: text/plain; charset="utf-8"

I am a little confused.  Are you referring to specimen bottles?  I was inquiring about what identifiers are being used on printed cassettes.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: Pratt, Caroline [mailto:Caroline.Pratt <@t> uphs.upenn.edu]
Sent: Thursday, August 16, 2012 9:47 AM
To: Ron; pamarcum <@t> uams.edu; Tom McNemar; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two identifiers...

It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey.  We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number.  It is a tremendous ongoing effort, but we are there now.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ron
Sent: Wednesday, August 15, 2012 12:39 PM
To: pamarcum <@t> uams.edu; tmcnemar <@t> lmhealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Two identifiers...

We do the same as Pam with the patient name and case number

Sent from my Verizon Wireless 4G LTE smartphone

"Marcum, Pamela A" <PAMarcum <@t> uams.edu> wrote:

>We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria.  We use the Thermo cassette writer and it transfers the information to the slides.
>
>Pam Marcum
>UAMS
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
>McNemar
>Sent: Wednesday, August 15, 2012 10:03 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Two identifiers...
>
>Hello all,
>
>For those of you using cassette and slide labelers....
>Does your system permit you to print the accession number, patient name, and  DOB on the face of the cassette?  Are you using the specimen number and name as your two identifiers?
>
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Health Systems
>(740) 348-4163
>(740) 348-4166
>tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
>www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Applica
>tion%20Data\Microsoft\Signatures\www.LMHealth.org>
>
>________________________________
>This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>Confidentiality Notice: This e-mail message, including any attachments,
>is for the sole use of the intended recipient(s) and may contain
>confidential and privileged information.  Any unauthorized review, use,
>disclosure or distribution is prohibited.  If you are not the intended
>recipient, please contact the sender by reply e-mail and destroy all
>copies of the original message.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

------------------------------

Message: 10
Date: Thu, 16 Aug 2012 07:01:11 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] marker specific  for mouse fibroblasts
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1345125671.55062.YahooMailNeo <@t> web130105.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello All!

I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts.

Kim

?
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

------------------------------

Message: 11
Date: Thu, 16 Aug 2012 10:02:07 -0400
From: "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu>
Subject: RE: [Histonet] RE: Two identifiers...
To: "Tom McNemar" <TMcNemar <@t> lmhealth.org>, "Ron"
        <pathrm35 <@t> comcast.net>, <pamarcum <@t> uams.edu>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <EFA78D6D81C0F348B8FA2880AE376CC346E71A <@t> uphmasphi015.UPHS.PENNHEALTH.PRV>

Content-Type: text/plain;       charset="utf-8"

I am so sorry to cause confusion, I am referring to specimen bottles, my apologies.

-----Original Message-----
From: Tom McNemar [mailto:TMcNemar <@t> lmhealth.org]
Sent: Thursday, August 16, 2012 9:51 AM
To: Pratt, Caroline; Ron; pamarcum <@t> uams.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two identifiers...

I am a little confused.  Are you referring to specimen bottles?  I was inquiring about what identifiers are being used on printed cassettes.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: Pratt, Caroline [mailto:Caroline.Pratt <@t> uphs.upenn.edu]
Sent: Thursday, August 16, 2012 9:47 AM
To: Ron; pamarcum <@t> uams.edu; Tom McNemar; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two identifiers...

It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey.  We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number.  It is a tremendous ongoing effort, but we are there now.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ron
Sent: Wednesday, August 15, 2012 12:39 PM
To: pamarcum <@t> uams.edu; tmcnemar <@t> lmhealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Two identifiers...

We do the same as Pam with the patient name and case number

Sent from my Verizon Wireless 4G LTE smartphone

"Marcum, Pamela A" <PAMarcum <@t> uams.edu> wrote:

>We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria.  We use the Thermo cassette writer and it transfers the information to the slides.
>
>Pam Marcum
>UAMS
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
>McNemar
>Sent: Wednesday, August 15, 2012 10:03 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Two identifiers...
>
>Hello all,
>
>For those of you using cassette and slide labelers....
>Does your system permit you to print the accession number, patient name, and  DOB on the face of the cassette?  Are you using the specimen number and name as your two identifiers?
>
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Health Systems
>(740) 348-4163
>(740) 348-4166
>tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
>www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Applica
>tion%20Data\Microsoft\Signatures\www.LMHealth.org>
>
>________________________________
>This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>Confidentiality Notice: This e-mail message, including any attachments,
>is for the sole use of the intended recipient(s) and may contain
>confidential and privileged information.  Any unauthorized review, use,
>disclosure or distribution is prohibited.  If you are not the intended
>recipient, please contact the sender by reply e-mail and destroy all
>copies of the original message.
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.

------------------------------

Message: 12
Date: Thu, 16 Aug 2012 08:51:32 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] marker specific  for mouse fibroblasts
To: 'Kim Merriam' <kmerriam2003 <@t> yahoo.com>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <14E2C6176416974295479C64A11CB9AE0162D07DAD12 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Kim

There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse.  I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse.  Acris has a lot of information on their website.  In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts.  Good Luck.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Thursday, August 16, 2012 8:01 AM
To: Histonet
Subject: [Histonet] marker specific for mouse fibroblasts

Hello All!

I am looking for a marker that is highly specific for mouse fibroblasts.  I have tried several markers, but none of them seem to be specific for ONLY fibroblasts.

Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 105, Issue 20
*****************************************