[Histonet] Eosin

Brendal Finlay brendal.finlay <@t> medicalcenterclinic.com
Fri Aug 10 07:00:40 CDT 2012

Do you recycle your alcohols?  If you do and you put the recycled
alcohols at the end (running down to xylene) it can GREATLY decolorize
the eosin in the H&E stain.  Also, make sure you have a good rinse
after the blueing step as that can mess with the eosin's pH which will
also decrease staining.

-----Original message-----
From: "Hannen, Valerie" Valerie.Hannen <@t> parrishmed.com
Date: Thu, 09 Aug 2012 09:23:26 -0500
To: histonet histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin

> Hi Folks...I am hoping you all give me a little help. Our
Pathologists are complaining about our Eosin on the H&E's being weak.
The funny thing is, is that it can go one
> day to the next...one day it looks great...the next it is weak!!
> I have already done some experimenting with...1) time tissue spends
in Eosin.... 2) making sure that the alcohols after Eosin are the
> reducing the time that the tissues spends in the alcohols atfer
Eosin... I have even gone as far as 4) increasing the rinse time in
water after the decolorizing and bluing
> steps. 5) I have checked the pH of the water as well.
> Any help and suggestions would greatly appreciated!!
> Thanks Gang!!
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.hannen <@t> parrishmed.com
> *
> "This email is intended solely for the use of the individual to
> whom it is addressed and may contain information that is
> privileged, confidentialor otherwise exempt from disclosure
> under applicable law. If the reader of this email is not the
> intended recipient or the employee or agent responsible for
> delivering the message to the intended recipient, you are
> hereby notified that any dissemination, distribution, or
> copying of this communication is strictly prohibited. If you
> have received this communication in error, please immediately
> delete this message. Thank you"
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.finlay <@t> medicalcenterclinic.com
phone - 850.474.8758
fax - 850.474.8584

More information about the Histonet mailing list