[Histonet] Eosin

[Brendal Finlay]

Do you recycle your alcohols?  If you do and you put the recycled
alcohols at the end (running down to xylene) it can GREATLY decolorize
the eosin in the H&E stain.  Also, make sure you have a good rinse
after the blueing step as that can mess with the eosin's pH which will
also decrease staining.



-----Original message-----
From: "Hannen, Valerie" Valerie.Hannen <@t> parrishmed.com
Date: Thu, 09 Aug 2012 09:23:26 -0500
To: histonet histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin

> Hi Folks...I am hoping you all give me a little help. Our
Pathologists are complaining about our Eosin on the H&E's being weak.
The funny thing is, is that it can go one
> 
> day to the next...one day it looks great...the next it is weak!!
> 
> I have already done some experimenting with...1) time tissue spends
in Eosin.... 2) making sure that the alcohols after Eosin are the
properconcentrations....3)
> 
> reducing the time that the tissues spends in the alcohols atfer
Eosin... I have even gone as far as 4) increasing the rinse time in
water after the decolorizing and bluing
> 
> steps. 5) I have checked the pH of the water as well.
> 
> Any help and suggestions would greatly appreciated!!
> 
> Thanks Gang!!
> 
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.hannen <@t> parrishmed.com
> 
> 
> 
> 
> 
> 
> 
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> 
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Brendal C. Finlay, HT (ASCP)
West Florida Medical Center Clinic
brendal.finlay <@t> medicalcenterclinic.com
phone - 850.474.8758
fax - 850.474.8584