[Histonet] sections falling off in day 2 ISH

[Megha Kumar]

Hello everyone!
When I subject murine skin and intestine paraffin sections (7-10microns) to
ISH protocol, the sections fall off on day 2 when I add the SSC. The
sections are taken on ploy lysine coated slides and remain on the slides
when I do other protocols such as antibody staining. Can anyone suggest me
why this is happening? How to prevent the slides from falling off on day 2?
Thank you for all the help!
regards
Megha



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